Abstract
Virescentia guangxiensis, a new species of Virescentia from Guangxi, South China, is described and illustrated based on morphological observations and phylogenetic analysis. This species was distinguished morphologically from other species by the presence of special expansion cells with a variable shape, obovoid, spherical, pear-shaped, located in the penultimate cells of primary or secondary fascicles, rarely terminal on primary fascicles, as well as by small whorls (250–350-µm wide) and short primary fascicles (5–7 cell stories). Phylogenetic analysis of molecular data from the rbcL and COI-5P loci supported the separation of the proposed new species from other species in the genus Virescentia. This is the first species of the order Batrachospermales reported in Guangxi and the second species of the genus Virescentia reported in China. This study expands the known species diversity and geographical distribution of freshwater Rhodophyta in China.
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Data Availability Statement
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Chapuis I S, Necchi O Jr, Zuccarello G C, Xie S L, Aboal M, Castillo P M S, Vis M L. 2017. A new genus, Volatus and four new species of Batrachospermum sensu stricto (Batrachospermales, Rhodophyta). Phycologia, 56(4): 454–468, https://doi.org/10.2216/16-73.1.
De Castro Agostinho D, Necchi O Jr. 2014. Systematics of the section Virescentia of the genus Batrachospermum (Batrachospermales, Rhodophyta) in Brazil. Phycologia, 53(6): 561–570, https://doi.org/10.2216/PH14-034.1.
Entwisle T J, Vis M L, Chiasson W B, Necchi O Jr, Sherwood A R. 2009. Systematics of the Batrachospermales (Rhodophyta)—a synthesis. Journal of Phycology, 45(3): 704–715, https://doi.org/10.1111/j.1529-8817.2009.00686.x.
Felsenstein J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17(6): 368–376, https://doi.org/10.1007/BF01734359.
Guindon S, Gascuel O. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52(5): 696–704, https://doi.org/10.1080/10635150390235520.
Hanyuda T, Suzawa Y, Suzawa T, Arai S, Sato H, Ueda K, Kumano S. 2004. Biogeography and taxonomy of Batrachospermum helminthosum (Batrachospermales, Rhodophyta) in Japan inferred from rbcL gene sequences. Journal of Phycology, 40(3): 581–588, https://doi.org/10.1111/j.1529-8817.2004.03159.x.
Kumano S. 2002. Freshwater Red Algae of the World. Biopress Ltd, Bristol, UK. 375p.
Necchi O Jr, De Castro Agostinho D, Vis M L. 2018. Revision of Batrachospermum section Virescentia (Batrachospermales, Rhodophyta) with the establishment of the new genus, Virescentia stat. nov. Cryptogamie, Algologie, 39(3): 313–338, https://doi.org/10.7872/crya/v39.iss3.2018.313.
Necchi O Jr, Garcia Filho A, Paiano M O. 2019a. Revision of Batrachospermum sections Acarposporophytum and Aristata (Batrachospermales, Rhodophyta) with the establishment of the new genera Acarposporophycos and Visia. Phytotaxa, 395(2): 51–65, https://doi.org/10.11646/phytotaxa.395.2.1.
Necchi O Jr, Garcia Fo. A S, Paiano M O, Vis M L. 2019b. Revision of Batrachospermum section Macrospora (Batrachospermales, Rhodophyta) with the establishment of the new genus Montagnia. Phycologia, 58(6): 582–591, https://doi.org/10.1080/00318884.2019.1624143.
Posada D, Buckley T R. 2004. Model selection and model averaging in phylogenetics: advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology, 53(5): 793–808, https://doi.org/10.1080/10635150490522304.
Ronquist F, Huelsenbeck J P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12): 1 572–1 574. https://doi.org/10.1093/bioinformatics/btg180
Rueness J. 2010. DNA barcoding of select freshwater and marine red algae (Rhodophyta). Cryptogamie, Algologie, 31(4): 377–386.
Salomaki E D, Kwandrans J, Eloranta P, Vis M L. 2014. Molecular and morphological evidence for Sheathia gen. nov. (Batrachospermales, Rhodophyta) and three new species. Journal of Phycology, 50(3): 526–542, https://doi.org/10.1111/jpy.12179.
Saunders G W. 1993. Gel purification of red algal genomic DNA: an inexpensive and rapid method for the isolation of polymerase chain reaction-friendly DNA. Journal of Phycology, 29(2): 251–254, https://doi.org/10.1111/j.0022-3646.1993.00251.x.
Saunders G W. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philosophical Transactions of the Royal Society B, 360(1462): 1 879–1 888, https://doi.org/10.1098/rstb.2005.1719.
Sheath R G, Vis M L. 2015. Red algae. In: Freshwater Algae of North America. Academic Press, San Diego, California. p.237–264.
Sheath R G, Vis M L, Cole K M. 1994. Distribution and systematics of Batrachospermum (Batrachospermales, Rhodophyta) in North America. 4. Section Virescentia. Journal of Phycology, 30(1): 108–117.
Shi Z X. 2006. Flora algarum sinicarum aquae dulcis, Tomus XIII, Rhodophyta, Phaeophyta. Science Press, Beijing, p.30–77. (in Chinese)
Sirodot S. 1873. Nouvelle classification des algues d’eau douce du genre Batrachospermum: développement; générations alternantes. Comptes-rendus hebdomadaires des séances de l’Académie des Sciences, 76: 1 216–1 120.
Sirodot S. 1884. Les Batrachospermes: Organisation, Fonctions, Développement, Classification. Librairie de l’Académie de Médecine, Paris, 299p.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2 731–2 739, https://doi.org/10.1093/molbev/msr121.
Thiers B. 2020. Index herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium, http://sweetgum.nybg.org/science/ih/. Accessed on 2020-07-31.
Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. 1997. The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25(24): 4 876–4 882, https://doi.org/10.1093/nar/25.24.4876.
Vis M L, Lee J, Eloranta P, Chapuis I S, Lam D W, Necchi O Jr. 2020. Paludicola gen. nov. and revision of the species formerly in Batrachospermum Section Turfosa (Batrachospermales, Rhodophyta). Journal of Phycology, 56(4): 844–861, https://doi.org/10.1111/jpy.13001.
Vis M L, Saunders G W, Sheath R G, Dunse K, Entwisle T J. 1998. Phylogeny of the Batrachospermales (Rhodophyta) inferred from rbcL and 18S ribosomal DNA gene sequences. Journal of Phycology, 34(2): 341–350, https://doi.org/10.1046/j.1529-8817.1998.340341.x.
Vis M L, Sheath R G. 1997. Biogeography of Batrachospermum gelatinosum (Batrachospermales, Rhodophyta) in North America based on molecular and morphological data. Journal of Phycology, 33(3): 520–526, https://doi.org/10.1111/j.0022-3646.1997.00520.x.
Vis M L, Entwisle T J. 2000. Insights into the phylogeny of the Batrachospermales (Rhodophyta) from rbcL sequence data of Australian taxa. Journal of Phycology, 36(6): 1 175–1 182, https://doi.org/10.1046/j.1529-8817.2000.99224.x.
Xie S L, Qiu M Y, Nan F R, Fang K P, Han J F. 2020. Batrachospermales (Rhodophyta) of China: a catalogue and bibliography. Nova Hedwigia, 110(1–2): 37–77, https://doi.org/10.1127/nova_hedwigia/2020/0565.
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Dr. E. W. Christina (The Pennsylvania State University) is acknowledged for the English editing.
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Supported by the National Natural Science Foundation of China (Nos. 31670208, 41871037, 31800172) and the Fund for Shanxi “1331 Project”
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Fang, K., Nan, F., Feng, J. et al. Virescentia guangxiensis (Batrachospermales, Rhodophyta): a new freshwater red algal species from South China. J. Ocean. Limnol. 39, 1538–1546 (2021). https://doi.org/10.1007/s00343-020-0225-0
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DOI: https://doi.org/10.1007/s00343-020-0225-0