Abstract
At present, the function and profile of long noncoding RNAs (IncRNAs) in half-smooth tongue soles Cynoglossus semilaevis remain poorly understood. Lherefore, we identified IncRNAs in the species using large-scale deep sequencing approaches. A total of 96 726 222 clean reads and 2497 IncRNAs were obtained from the compound samples. In total, 1 694 known IncRNAs and 803 novel IncRNAs were identified. Most of the novel IncRNAs distributed mainly in a range of 200–3 000 nt and contained 2–6 exons. Six novel IncRNAs were selected for expression analysis by quantitative reverse transcription-polymerase chain reaction, of which lnc_770 and lnc_150 were expressed mainly in the ovary. Lhis study provides a valuable resource for IncRNA studies of half-smooth tongue sole and improves our understanding of the function of non-coding RNA in fish.
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Supported by the Shandong Provincial Natural Science Foundation (No. ZR2013CL003), the National Natural Science Foundation of China (Nos. 81670398, 91639102), and the funding of Taishan Scholars of Shandong Province to Binzhou Medical University and the Key Research Project of Yantai City (No. 2018ZHGY071)
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Jiang, X., Jing, X., Lü, Y. et al. Genome-wide identification and prediction of long non-coding RNAs in half-smooth tongue sole Cynoglossus semilaevis. J. Ocean. Limnol. 38, 226–235 (2020). https://doi.org/10.1007/s00343-019-8287-6
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DOI: https://doi.org/10.1007/s00343-019-8287-6