Abstract
Five full-sib families of the Pacific oyster (Crassostrea gigas) larvae were used to study the mode of inheritance at eight microsatellite loci, and the feasibility of these markers for kinship estimate was also examined. All eight microsatellite loci were compatible with Mendelian inheritance. Neither evidence of sex-linked barriers to transmission nor evidence of major barriers to fertilization between gametes from the parents was shown. Three of the eight loci showed the presence of null alleles in four families, demonstrating the need to conduct comprehensive species-specific inheritance studies for microsatellite loci used in population genetic studies. Although the null allele heterozygotes were considered as homozygotes in the calculation of genetic distance, offspring from five full-sib families were unambiguously discriminated in the neighbor-joining dendrogram. This result indicates that the microsatellite markers may be capable of discriminating between related and unrelated oyster larvae in the absence of pedigree information, and is applicable to the investigation of the effective number of parents contributing to the hatchery population of the Pacific oyster.
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References
Allendorf, F. W. and S. R. Phelps, 1980. Loss of genetic variation in a hatchery stock of cutthroat trout. Trans. Am. Fish. Soc. 109: 537–543.
Banks, M. A., M. S. Blouin, B. A. Baldwin, V. K. Rashbrook, H. A. Fitzgerald, S. M. Blankenship and D. Hedgecock, 1999. Isolation and inheritance of novel microsatellites in chinook salmon (Oncorhynchus tschawytscha). J. Hered. 90: 281–288.
Blouin, M. S., 2003. DNA-based methods for pedigree reconstruction and kinship analysis in natural populations. Trends Ecol. Evol. 18: 503–511.
Bowcock, A. M., A. Ruiz-Linares, J. Tomfohrde, E. Minch, J. R. Kidd and L. L. Cavalli-Sforza, 1994. High resolution of human evolution with polymorphic microsatellites. Nature 368: 455–457.
Callen, D. F., A. D. Thompson, Y. Shen, H. A. Phillips, R. I. Richards, J. C. Mulley and G. R. Sutherland, 1993. Incidence and origin of “null” alleles in the (AC)n microsatellite markers. Am. J. Hum. Genet. 52: 922–927.
DOF (Department of Fisheries), 2003. China Fisheries Statistic Yearbook 2002. China Agriculture Press, Beijing. (in Chinese)
FAO (Food and Agriculture Organization), 2006. World aquaculture production of fish, crustaceans, molluscs, etc., by principal species (ftp://ftp.fao.org/FI/STAT/summary/default.htm#aqua)
Felsenstein, J., 2000. PHYLIP version 3.6. Department of Genetics, University of Washington, Seattle, WA, USA
Gullberg, A., H. Tegelstrom and M. Olsson, 1997. Microsatellites in the sand lizard (Lacerta agilis): Description, variation, inheritance, and applicability. Biochem. Genet. 35: 281–295.
Hadrys, H., M. Balick and B. Schierwater, 1992. Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol. Ecol. 1: 55–63.
Hurst, L. D., A. Atlan and B. O. Bengtsson, 1996. Genetic conflicts. Q. Rev. Biol. 71: 317–364.
Huvet, A., P. Boudry, M. Ohresser, C. Delsert and F. Bonhomme, 2000. Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species. Anim. Genet. 31: 71–72.
Jones, A. G., C. A. Stockwell, D. Walker and J. C. Avise, 1998. The molecular basis of a microsatellite null allele from the white sands pupfish. J. Hered. 89: 339–342.
Launey, S. and D. Hedgecock, 2001. High genetic load in the Pacific oyster Crassostrea gigas. Genetics 159: 255–265.
Li, Q., C. Park and A. Kijima, 2003a. Allelic transmission of microsatellites and application to kinship analysis in newly hatched Pacific abalone larvae. Fish. Sci. 69: 883–889.
Li, Q., C. Park, T. Kobayashi and A. Kijima, 2003b. Inheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannai. Mar. Biotechnol. 5: 331–338.
Magoulas, A., B. Ghjetvaj, V. Terzoglou and E. Zouros, 1998. Three polymorphic microsatellites in the Japanese oyster, Crassostrea gigas (Thunberg). Anim. Genet. 29: 69–70.
McGoldrick, D.J. and D. Hedgecock, 1997. Fixation, segregation, and linkage of allozymes in inbred families of the Pacific oyster Crassostrea gigas: Implications for the causes of inbreeding depression. Genetics 146: 321–344.
McGoldrick, D., D. Hedgecock, L. J. English, P. Baoprasertkul and R. D. Ward, 2000. The transmission of microsatellite alleles in Australian and North American stocks of the Pacific oyster (Crassostrea gigas): Selection and null alleles. J. Shellfish Res. 19: 779–788.
Norris, A. T., D. G. Bradley and E. P. Cunningham, 2000. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture 182: 73–83.
Selvamani, M. J. P., S. M. Degnan and B. M. Degnan, 2001. Microsatellite genotyping of individual abalone larvae: Parentage assignment in aquaculture. Mar. Biotechnol. 3: 478–485.
Sugaya, T., M. Ikeda, H. Mori and N. Taniguchi, 2002. Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicus. Fish. Sci. 68: 299–305.
Tautz, D., 1989. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl. Acids Res. 17: 6 463–6 471.
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Supported by the National High Technology Research and Development Program of China (863 Program, No. 2006AA10A409), and the National Natural Science Foundation of China (No. 30571442)
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Li, Q., Zheng, X. & Yu, R. Inheritance mode of microsatellite loci and their use for kinship analysis in the Pacific oyster (Crassostrea gigas). Chin. J. Ocean. Limnol. 26, 256–262 (2008). https://doi.org/10.1007/s00343-008-0256-4
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DOI: https://doi.org/10.1007/s00343-008-0256-4