Skip to main content
SpringerLink
Log in

Inheritance of Microsatellite DNA Markers in the Pacific Abalone Haliotis discus hannai

  • Published:
Marine Biotechnology Aims and scope Submit manuscript

Abstract

Microsatellite markers have been developed for a variety of abalones, and locus-specific homozygote excesses at population level have been recorded for microsatellite loci. To ascertain whether null alleles exist at microsatellite loci in the Pacific abalone, we studied the mode of inheritance of 7 microsatellite loci in 4 families with a reciprocal cross of 2 females × 2 males. All loci segregated codominantly, but only 3 loci (Hdh1321, Hdh78, and Hdd108C) conformed to Mendelian segregation and can be used for parental analysis and population genetic studies. When null alleles were considered, 2 loci (Hdh1761 and Hdh1457) confirmed Mendelian expectations in all families, while the remaining 2 loci (Hdd114B and Hdd229) showed deviation from Mendelian segregation in at least one family even though null alleles were considered. These results indicated the need to test the inheritance pattern for microsatellite markers in abalones before using them for population genetic or parentage analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1

References

  • W.R. Ardren S. Borer F. Thrower J.E. Joyce A.R. Kapuscinski (1999) ArticleTitleInheritance of 12 microsatellite loci in Oncorhynchus mykiss. J Hered 90 529–536 Occurrence Handle10.1093/jhered/90.5.529 Occurrence Handle1:CAS:528:DyaK1MXntlWmtLo%3D

    Article  CAS  Google Scholar 

  • M.A. Banks M.S. Blouin B.A. Baldwin V.K. Rashbrook H.A. Fitzgerald S.M. Blankenship D. Hedgecock (1999) ArticleTitleIsolation and inheritance of novel microsatellites in chinook salmon (Oncorhynchus tschawytscha). J Hered 90 281–288 Occurrence Handle10.1093/jhered/90.2.281 Occurrence Handle1:CAS:528:DyaK1MXhs1yktL4%3D

    Article  CAS  Google Scholar 

  • A.R. Beaumont C.M. Beveridge M.D. Budd (1983) ArticleTitleSelection and heterozygosity within single families of the mussel, Mytilus edulis (L. Mar Biol 4 151–161

    Google Scholar 

  • P. Bentzen J.B. Olsen J.E. McLean T.R. Seamons T.R. Quinn (2001) ArticleTitleKinship analysis of Pacific salmon: insights into mating, homing, and timing of reproduction. J Hered 92 127–136 Occurrence Handle10.1093/jhered/92.2.127 Occurrence Handle1:STN:280:DC%2BD38%2Fht1Wktg%3D%3D Occurrence Handle11396570

    Article  CAS  PubMed  Google Scholar 

  • D.F. Callen A.D. Thompson Y. Shen H.A. Phillips R.I. Richards J.C. Mulley G.R. Sutherland (1993) ArticleTitleIncidence and origin of “null” alleles in the (AC)n microsatellite markers. Am J Hum Genet 52 922–927

    Google Scholar 

  • N. Conod J.R. Bartlett B.S. Evans N.G. Elliott (2002) ArticleTitleComparison of mitochondrial and nuclear DNA analyses of population structure in the blacklip abalone Haliotis rubra Leach. Mar Freshw Res 53 711–718 Occurrence Handle10.1071/MF01197 Occurrence Handle1:CAS:528:DC%2BD38Xmtlylu7o%3D

    Article  CAS  Google Scholar 

  • D.J. Day P.W. Speiser E. Schulze M. Bettendorf J. Fitness F. Barany R.C. White (1996) ArticleTitleIdentification of non-amplifying CYP21 genes when using PCR-based diagnosis of 21-hydroxylase deficiency in congenital adrenal hyperplasia (CAH) affected pedigrees. Hum Mol Genet 5 2039–2048 Occurrence Handle10.1093/hmg/5.12.2039 Occurrence Handle1:CAS:528:DyaK28XnsFOgsLg%3D Occurrence Handle8968761

    Article  CAS  PubMed  Google Scholar 

  • F.P.M. de Villena C. Sapienza (2001) ArticleTitleNonrandom segregation during meiosis: the unfairness of females. Mamm Genome 12 331–339 Occurrence Handle11331939

    PubMed  Google Scholar 

  • H. Ellegren (2000) ArticleTitleMicrosatellite evolution: a battle between replication slippage and point mutation. Trends Genet 18 70 Occurrence Handle10.1016/S0168-9525(02)02631-8

    Article  Google Scholar 

  • B. Evans R.W.G. White N.G. Elliott (2000) ArticleTitleCharacterization of microsatellite loci in the Australian blacklip abalone (Haliotis rubra, Leach). Mol Ecol 9 1183–1184 Occurrence Handle10.1046/j.1365-294x.2000.00954-8.x Occurrence Handle1:CAS:528:DC%2BD3cXmsVWgsrc%3D Occurrence Handle10964244

    Article  CAS  PubMed  Google Scholar 

  • B. Evans N. Conod N.G. Elliott (2001) ArticleTitleEvaluation of microsatellite primer conservation in abalone. J Shellfish Res 20 1065–1070

    Google Scholar 

  • K. Fujino (1978) ArticleTitleGenetic studies on the Pacific abalone, I: inbreeding and overdominance as evidenced by biochemical polymorphism in a wild population. Nippon Suisan Gakkaishi 44 357–361

    Google Scholar 

  • Y. Fujio A. Yuzawa S. Kikuchi A. Koganezawa (1986) ArticleTitleGenetic study on the population structure of abalone. Bull Tohoku Reg Fish Res Lab 48 59–65

    Google Scholar 

  • D.W. Foltz (1986) ArticleTitleSegregation and linkage studies of allozyme loci in pair crosses of the oyster Crassostrea virginica. Biochem Genet 24 941–956 Occurrence Handle1:CAS:528:DyaL2sXmsVGltQ%3D%3D Occurrence Handle3800871

    CAS  PubMed  Google Scholar 

  • P.M. Gaffney T.M. Scott (1984) ArticleTitleGenetic heterozygosity and production traits in natural and hatchery populations of bivalves. Aquaculture 42 289–392

    Google Scholar 

  • A. Gullberg H. Tegelstrom M. Olsson (1997) ArticleTitleMicrosatellites in the sand lizard (Lacerta agilis): description, variation, inheritance, and applicability. Biochem Genet 35 281–295 Occurrence Handle10.1023/A:1021801217185 Occurrence Handle1:CAS:528:DyaK1cXhsV2ktg%3D%3D Occurrence Handle9435947

    Article  CAS  PubMed  Google Scholar 

  • B.S. Holland (2001) ArticleTitleInvasion without a bottleneck: microsatellite variation in natural and invasive populations of the brown mussel Perna perna (L). Mar Biotechnol 3 407–415 Occurrence Handle10.1007/s1012601-0060-Z Occurrence Handle1:CAS:528:DC%2BD3MXpt1Sgs7o%3D

    Article  CAS  Google Scholar 

  • L.E. Holm V. Loeschcke C. Bendixen (2001) ArticleTitleElucidation of the molecular basis of a null allele in a rainbow trout microsatellite. Mar Biotechnol 3 555–560 Occurrence Handle10.1007/s10126-001-0057-7 Occurrence Handle1:CAS:528:DC%2BD38XotVWhtA%3D%3D

    Article  CAS  Google Scholar 

  • B. Huang P.J. Hanna (1998) ArticleTitleIdentification of three polymorphic microsatellite loci in blacklip abalone, Haliotis rubra (Leach), and detection in other abalone species. J Shellfish Res 17 795–799

    Google Scholar 

  • G.D.D. Hurst M. Schilthuizen (1998) ArticleTitleSelfish genetic elements and speciation. Heredity 80 2–8 Occurrence Handle10.1038/sj.hdy.6883370

    Article  Google Scholar 

  • L.D. Hurst A. Atlan B.O. Bengtsson (1996) ArticleTitleGenetic conflicts. Q Rev Biol 71 317–364 Occurrence Handle1:STN:280:BymH38rovFE%3D Occurrence Handle8828237

    CAS  PubMed  Google Scholar 

  • A.G. Jones C.A. Stockwell D. Walker J.C. Avise (1998) ArticleTitleThe molecular basis of a microsatellite null allele from the white sands pupfish. J Hered 89 339–342 Occurrence Handle1:CAS:528:DyaK1cXls1aqtLc%3D

    CAS  Google Scholar 

  • A. Kijima T. Furutono I. Kawahara T. Noro M. Omori Y. Fujio (1997) ArticleTitleA mode of inheritance for isozymic variation and detection of a null allele at Pgm-1 and Pgm-2 in the Pacific abalone by mating experiment. Fish Genet Breed Sci 25 73–80

    Google Scholar 

  • V.L. Kirby R. Villa D.A. Powers (1998) ArticleTitleIdentification of microsatellites in the California red abalone, Haliotis rufescens. J Shellfish Res 17 801–804

    Google Scholar 

  • E.W. Knapik A. Goodman M. Ekker M. Chevrette J. Delgado S. Neuhauss N. Shimoda W. Driever M.C. Fishman H.J. Jacob (1998) ArticleTitleA microsatellite genetic linkage map for zebrafish (Danio rerio). Nat Genet 18 338–343 Occurrence Handle1:CAS:528:DyaK1cXit1egsro%3D Occurrence Handle9537415

    CAS  PubMed  Google Scholar 

  • S. Launey D. Hedgecock (2001) ArticleTitleHigh genetic load in the Pacific oyster Crassostrea gigas. Genetics 159 255–265

    Google Scholar 

  • Q. Li C. Park A. Kijima (2002) ArticleTitleIsolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai. J Shellfish Res 21 811–815

    Google Scholar 

  • T.W. Lyttle (1991) ArticleTitleSegregation distorters. Annu Rev Genet 25 511–557 Occurrence Handle1:CAS:528:DyaK38XitVyltb4%3D Occurrence Handle1812815

    CAS  PubMed  Google Scholar 

  • D.J. McGoldrick D. Hedgecock (1997) ArticleTitleFixation, segregation and linkage of allozyme loci in inbred families of the Pacific oyster Crassostrea gigas (Thunberg): implication for the causes of inbreeding depression. Genetics 146 321–334

    Google Scholar 

  • D.J. McGoldrick D. Hedgecock L.J. English P. Baoprasertkul R.D. Ward (2000) ArticleTitleThe transmission of microsatellite alleles in Australian and North American stocks of the Pacific oyster (Crassostrea gigas): Selection and null alleles. J Shellfish Res 19 779–788

    Google Scholar 

  • K.M. Miller K. Laberee K.H. Kaukinen S. Li R.E. Withler (2001) ArticleTitleDevelopment of microsatellite loci in pinto abalone (Haliotis kamtschatkana). Mol Ecol Notes 1 315–317 Occurrence Handle1:CAS:528:DC%2BD38XltVGqtw%3D%3D

    CAS  Google Scholar 

  • A. Norris D.G. Bradley E.P. Cunningham (2000) ArticleTitleParentage and relatedness determination in farmed Atlantic salmon (Salmo solar) using microsatellite markers. Aquaculture 182 73–83 Occurrence Handle10.1016/S0044-8486(99)00247-1

    Article  Google Scholar 

  • J.M. Pemberton J. Slate D.R. Bancroft J.A. Barrett (1995) ArticleTitleNonamplifying alleles at microsatellite loci: a caution for parentage and population studies. Mol Ecol 4 249–252 Occurrence Handle1:STN:280:ByqB2Mzkslw%3D Occurrence Handle7735527

    CAS  PubMed  Google Scholar 

  • D.C. Queller J.E. Strassmann C.R. Hughes (1993) ArticleTitleMicrosatellites and kinship. Trends Ecol Evol 8 285–288

    Google Scholar 

  • T. Sakamoto R.G. Danzmann K. Gharbi P. Howard A. Ozaki S.K. Khoo R.A. Woram N. Okamoto M.M. Ferguson L.E. Holm R. Guyomard B. Hoyheim (2000) ArticleTitleA microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics 155 1331–1345 Occurrence Handle1:CAS:528:DC%2BD3cXlsFSku7Y%3D Occurrence Handle10880492

    CAS  PubMed  Google Scholar 

  • C. Schlotterer D. Tautz (1992) ArticleTitleSlippage synthesis of simple sequence DNA. Nucleic Acids Res 20 211–215 Occurrence Handle1741246

    PubMed  Google Scholar 

  • M. Sekino M. Hara (2001) ArticleTitleMicrosatellite DNA loci in Pacific abalone Haliotis discus discus (Mollusca, Gastropoda, Haliotidae). Mol Ecol Notes 1 8–10 Occurrence Handle1:CAS:528:DC%2BD3MXlslaksrY%3D

    CAS  Google Scholar 

  • M.J.P. Selvamani S.M. Degnan D. Paetkau B.M. Degnan (2000) ArticleTitleHighly polymorphic microsatellite loci in the Heron Reef population of the tropical abalone Haliotis asinina. Mol Ecol 9 1184–1186 Occurrence Handle10.1046/j.1365-294x.2000.00954-9.x Occurrence Handle1:CAS:528:DC%2BD3cXmsVWgs74%3D Occurrence Handle10964245

    Article  CAS  PubMed  Google Scholar 

  • M.J.P. Selvamani S.M. Degnan B.M. Degnan (2001) ArticleTitleMicrosatellite genotyping of individual abalone larvae: Parentage assignment in aquaculture. Mar Biotechnol 3 478–485 Occurrence Handle10.1007/s1012601-0062-X Occurrence Handle1:CAS:528:DC%2BD3MXpt1SgsLw%3D

    Article  CAS  Google Scholar 

  • T. Sugaya M. Ikeda H. Mori N. Taniguchi (2002) ArticleTitleInheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicus. Fisheries Set 68 299–305 Occurrence Handle10.1046/j.1444-2906.2002.00425.x Occurrence Handle1:CAS:528:DC%2BD38XjvV2qtLk%3D

    Article  CAS  Google Scholar 

  • Y.K. Tam I. Kornfield (1996) ArticleTitleCharacterization of microsatellite markers in Homarus (Crustacea, Decapoda). Mol Mar Biol Biotechnol 5 230–238 Occurrence Handle1:CAS:528:DyaK28Xls1yjt7Y%3D Occurrence Handle8817929

    CAS  PubMed  Google Scholar 

  • J.S. Taylor J.S.P. Sanny F. Breden (1999) ArticleTitleMicrosatellite allele size homoplasy in the guppy (Poecilia reticulata). J Mol Evol 48 245–247 Occurrence Handle1:CAS:528:DyaK1MXotVyqtg%3D%3D Occurrence Handle9929393

    CAS  PubMed  Google Scholar 

  • I.A. Van Pijlen B. Amos T. Burke (1995) ArticleTitlePatterns of genetic variability at individual minisatellite loci in Minke whale Balaenoptera acutorostrata populations from three different oceans. Mol Biol Evol 12 459–472 Occurrence Handle1:CAS:528:DyaK2MXltF2ntbY%3D Occurrence Handle7739388

    CAS  PubMed  Google Scholar 

  • Y. Vigouroux J.S. Jaqueth Y. Matsuoka O.S. Smith W.D. Beavis J.S.C. Smith J. Doebley (2002) ArticleTitleRate and pattern of mutation at microsatellite loci in maize. Mol Biol Evol 19 1251–1260 Occurrence Handle1:CAS:528:DC%2BD38XmtF2ku7c%3D Occurrence Handle12140237

    CAS  PubMed  Google Scholar 

  • J.L. Weber C. Wong (1993) ArticleTitleMutation of human short tandem repeats. Hum Mol Genet 2 1123–1128 Occurrence Handle1:CAS:528:DyaK3sXmsValsrY%3D Occurrence Handle8401493

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The study was supported by grants from the Japan Society for the Promotion of Science, Agriculture, Forestry and Fisheries Research Council of Japan, and National Natural Science Foundation of China (No. 30170735).

Author information

Authors and Affiliations

  1. Fisheries College, Ocean University of China, Qingdao 266003, China

    Qi Li

  2. Education and Research Center of Marine Bioresources, Graduate School of Agricultural Science, Tohoku University, Onagawa, Oshika 986-2242, Japan

    Choulji Park & Akihiro Kijima

  3. Iwate Fisheries Technology Center, Heita, Kamaishi 026, Japan

    Toshimasa Kobayashi

Authors
  1. Qi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Choulji Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshimasa Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akihiro Kijima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Akihiro Kijima.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, Q., Park, C., Kobayashi, T. et al. Inheritance of Microsatellite DNA Markers in the Pacific Abalone Haliotis discus hannai . Mar. Biotechnol. 5, 331–338 (2003). https://doi.org/10.1007/s10126-002-0116-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10126-002-0116-8

Keywords

Search

Navigation