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Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus

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Abstract

The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 (SPsn25) by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831bp contains a CDS of 615 bp, coding 204 amino acid residues, and a 5 UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.

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Abbreviations

SPsn25:

SNAP-25 of sea perch

SNAP-25:

syaptosome-associated protein of 25 kDa

SNARE:

soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor

t-SNARE:

target-membrane SNARE

CDS:

coding sequence

UTR:

untranslated region

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Chen Kui  (陈魁)

  2. The First Institute of Oceanography, State Oceanic Administration, Oingdao, 266061, China

    Chen Kui  (陈魁), Huang Xiaohang  (黄晓航) & Chai Yingmei  (柴迎梅)

  3. Graduate University, Chinese Academy of Sciences, Beijing, 100039, China

    Chen Kui  (陈魁)

  4. Department of Physiology, University of Toronto, Toronto, M5S 1A8, Canada

    Herbert Y. Gaisano

Authors
  1. Chen Kui  (陈魁)
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  2. Huang Xiaohang  (黄晓航)
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  3. Chai Yingmei  (柴迎梅)
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  4. Herbert Y. Gaisano
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Corresponding author

Correspondence to Huang Xiaohang  (黄晓航).

Additional information

Supported by the NSFC (No.40476060) and Hi-Tech Research and Development Program of China (No. 2002AA629120).

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Chen, K., Huang, X., Chai, Y. et al. Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus . Chin. J. Ocean. Limnol. 25, 378–385 (2007). https://doi.org/10.1007/s00343-007-0378-0

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  • DOI: https://doi.org/10.1007/s00343-007-0378-0

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