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The preparation of low electroendosmosis agarose and its physico-chemical property

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Abstract

Studies on Gelidium amansii agar fractionations were carried out in this paper. Gelidium amansii agar was fractionated on DEAE-Cellulose, and four fractions were obtained sequentially. The fractions were analyzed on physical and chemical properties, and IR and 13C-NMR spectroscopy applied for elucidating the chemical structure. Among the four fractions obtained, water fraction measured up to the standard of low EEO agarose. The sulfate content, ash content, electroendosmosis and gel strength (1%) of water fraction were 0.16%, 0.34%, 0.12 and 1 130g/cm2 respectively, similar to those of the Sigma products.

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Correspondence to Zhang Quanbin  (张全斌).

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Supported by, the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209).

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Hu, R., Liu, X., Liu, L. et al. The preparation of low electroendosmosis agarose and its physico-chemical property. Chin. J. Ocean. Limnol. 25, 367–372 (2007). https://doi.org/10.1007/s00343-007-0367-3

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  • DOI: https://doi.org/10.1007/s00343-007-0367-3

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