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Pulsed laser deposition of lysozyme: the dependence on shot numbers and the angular distribution

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Abstract

The ejection of molecules from a pressed solid target of lysozyme induced by laser ablation in the UV-regime at a wavelength of 355 nm was investigated. The ablation studies were carried out in vacuum at a laser fluence of 2 J/cm2 for which a significant fraction of proteins remains intact. This was verified by matrix-assisted laser desorption ionization (MALDI) spectrometry of thin films deposited on silicon substrates. The deposition rate of lysozyme was found to decrease with the number of shots and was correlated with increasing thermal damage of the lysozyme. This was monitored by measurements of the optical reflectivity of dry lysozyme. The angular distribution of the mass deposition can be fitted well by Anisimov’s hydrodynamic model. The total deposited yield over the entire hemisphere from direct laser ablation of lysozyme was estimated from this model and found to be three orders of magnitude less than the ablated mass.

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Acknowledgments

The authors acknowledge the competent technical assistance from Arne Nordskov and Lotte Nielsen. We would also like to acknowledge Dennis D. Corell and Carsten Dam-Hansen for fruitful collaboration and support on reflectance measurements using the integrating sphere.

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Authors and Affiliations

  1. National Institute for Lasers, Plasma and Radiation Physics, Atomistilor 409, 77125, Bucharest-Magurele, Romania

    C. Constantinescu, A. Matei & M. Dinescu

  2. Department of Photonics Engineering, Technical University of Denmark, 4000, Roskilde, Denmark

    J. Schou & S. Canulescu

Authors
  1. C. Constantinescu
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  2. A. Matei
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  3. J. Schou
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  4. S. Canulescu
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  5. M. Dinescu
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Correspondence to J. Schou.

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Constantinescu, C., Matei, A., Schou, J. et al. Pulsed laser deposition of lysozyme: the dependence on shot numbers and the angular distribution. Appl. Phys. B 113, 367–371 (2013). https://doi.org/10.1007/s00340-013-5485-3

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  • DOI: https://doi.org/10.1007/s00340-013-5485-3

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