Abstract
The present investigation focuses on the synthesis and characterization of Ce1−xEuxO2 (X = 0, 0.02, 0.04, 0.06, and 0.08) materials. The Eu-doped CeO2 compounds were prepared using the chemical precipitation method. Powder X-ray diffraction (XRD) analysis confirmed the phase-pure Cubic structured CeO2 crystal system. Structural examination of the synthesized materials was conducted through the Rietveld refinement approach. Scanning electron microscopy (SEM) along with energy dispersive spectroscopy (EDS) was employed to evaluate surface morphology and elemental composition (EDAX) of the materials. FTIR spectroscopy revealed the presence of vibrational linkages associated with distinct functional groups. The energy gap of the synthetic materials was investigated using UV–Vis spectroscopy. Photoluminescence (PL) analysis demonstrated the luminescence activity of the manufactured materials, which emitted light-green fluorescence at approximately 516 nm upon excitation at a wavelength of 380 nm. Moreover, the electron density distribution in the fabricated materials was successfully determined. The highest covalent nature of the Ce–O bond and single unit cells were observed in the 4% Eu-substituted CeO2. Antibacterial activity of the synthetic materials was tested against Aeromonas hydrophila and Streptococcus pyogenes. Additionally, a hemolysis inquiry was conducted to assess the breakdown of human red blood cells. Based on all experimental findings, it was observed that the CeO2 host lattice showed optimal characteristics at 4% Eu dopant concentration. This work offers a promising avenue for the development of effective materials for various biological applications.
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References
A. Muthuvel et al., Microwave-assisted green synthesis of nanoscaled titanium oxide: photocatalyst, antibacterial and antioxidant properties. J. Mater. Sci. Mater. Electron. 32, 23522–23539 (2021)
N. Al-Zaqri et al., Green synthesis of nickel oxide nanoparticles and its photocatalytic degradation and antibacterial activity. J. Mater. Sci. Mater. Electron. 33, 11864–11880 (2022)
L. Jintong et al., Homologous metal–organic framework hybrid as tandem catalyst for enhanced therapy against hypoxic tumor cells. Angew. Chem. 131, 7890–7894 (2019)
R. Javed et al., Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects. J. Nanobiotechnol. 18, 172 (2020)
A.S. Fauci, D.M. Morens, The perpetual challenge of infectious diseases. N. Engl. J. Med. 366, 454–461 (2012). https://doi.org/10.1056/nejmra1108296
O. Aisida et al., Bio-inspired encapsulation and functionalization of iron oxide nanoparticles for biomedical applications. Eur. Polym. J. (2020). https://doi.org/10.1016/j.eurpolymj.2019.109371
R. Gaynes, The discovery of penicillin—new insights after more than 75 years of clinical use. Emerg. Infect. Dis. 23, 849–853 (2017). https://doi.org/10.3201/eid2305.161556
A. Nigam, D. Gupta, A. Sharma, Treatment of infectious disease: beyond antibiotics. Microbiol. Res. 169, 643–651 (2014). https://doi.org/10.1016/j.micres.2014.02.009
V.M. D’Costa, C.E. King, L. Kalan, M. Morar, W.W.L. Sung, C. Schwarz et al., Antibiotic resistance is ancient. Nature 477, 457–461 (2011). https://doi.org/10.1038/nature10388
M. Frieri, K. Kumar, A. Boutin, Antibiotic resistance. J. Infect. Public Health 10, 369–378 (2017). https://doi.org/10.1016/j.jiph.2016.08.007
L.R. Khot, S. Sankaran, J.M. Maja, R. Ehsani, E.W. Schuster, Applications of nanomaterials in agricultural production and crop protection: a review. Crop Prot. 35, 64–70 (2012). https://doi.org/10.1016/j.cropro.2012.01.007
S. Das, B. Sen, N. Debnath, Recent trends in nanomaterials applications in environmental monitoring and remediation. Environ. Sci. Pollut. Res. 22, 18333–18344 (2015). https://doi.org/10.1007/s11356-015-5491-6
K. Scida, P.W. Stege, G. Haby, G.A. Messina, C.D. García, Recent applications of carbon-based nanomaterials in analytical chemistry: critical review. Anal. Chim. Acta 691, 6–17 (2011). https://doi.org/10.1016/j.aca.2011.02.025
S. Chaudhary, S.K. Mehta, Selenium nanomaterials: applications in electronics, catalysis and sensors. J. Nanosci. Nanotechnol. 14, 1658–1674 (2014). https://doi.org/10.1166/jnn.2014.9128
S. Mitragotri, D.G. Anderson, X. Chen, E.K. Chow, D. Ho, A.V. Kabanov et al., Accelerating the translation of nanomaterials in biomedicine. ACS Nano 9, 6644–6654 (2015). https://doi.org/10.1021/acsnano.5b03569
G. Franci, A. Falanga, S. Galdiero, L. Palomba, M. Rai, G. Morelli et al., Silver nanoparticles as potential antibacterial agents. Molecules 20, 8856–8874 (2015). https://doi.org/10.3390/molecules20058856
Y. Rodhe, S. Skoglund, I. OdnevallWallinder, Z. Potácová, L. Möller, Copper based nanoparticles induce high toxicity in leukemic HL60 cells. Toxicol. Vitro 29, 1711–1719 (2015). https://doi.org/10.1016/j.tiv.2015.05.020
A. Ivask, K. Juganson, O. Bondarenko, M. Mortimer, V. Aruoja, K. Kasemets et al., Mechanisms of toxic action of Ag, ZnO and CuO nanoparticles to selected ecotoxicological test organisms and mammalian cells in vitro: a comparative review. Nanotoxicology 8, 57–71 (2014). https://doi.org/10.3109/17435390.2013.855831
G. Kamarajan et al., Green synthesis of ZnO nanoparticles using Acalypha indica leaf extract and their photocatalyst degradation and antibacterial activity. J. Indian Chem. Soc. 99, 100695 (2022)
A. Sirelkhatim, S. Mahmud, A. Seeni, N.H.M. Kaus, L.C. Ann, S.K.M. Bakhori et al., Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. NanoMicro Lett. 7, 219–242 (2015). https://doi.org/10.1007/s40820-015-0040-x
A. ÁvalosFúnez, A. Isabel Haza, D. Mateo, P. Morales, In vitro evaluation of silver nanoparticles on human tumoral and normal cells. Toxicol. Mech. Method 23, 153–160 (2013). https://doi.org/10.3109/15376516.2012.762081
M. Mogensen, N. Sammes, G. Tompsett, Solid State Ion. 129, 63 (2000)
S. Tsunekawa, T. Fukuda, A.J. Kasuya, Appl. Phys. 87, 1318 (2000)
X. Feng, D. Sayle, Z. Wang, M. Paras, B. Santora, A. Sutorik, T. Sayle, Y. Yang, Y. Ding, X. Wang, Y. Her, Science 312, 1504 (2006)
S. Patil, S. Reshetnikov, M. Haldar, S.J. Seal, Phys. Chem. C 111, 8437 (2007)
L. Li, J. Tao, H. Pan, H. Chen, X. Wu, F. Zhu, X. Xu, R.J. Tang, Mater. Chem. 18, 5363 (2008)
X. Wen, M. Li, Y. Wang, J. Zhang, L. Fu, R. Hao, Y. Ma, X. Ai, Langmuir 24, 6932 (2008)
I. Hemmila, V.M. Mukkala, H.J. Takalo, Alloys Compd. 249, 158 (1997)
J. Wu, G.L. Wang, D.Y. Jin, J.L. Yuan, Y.F. Guan, J. Piper, Chem. Commun. 3, 365 (2008)
A. Kumar, S. babu, A. Karaoti, A. Schulte, S. Seal, Langmuir. 25(18), 10998–11007 (2009). https://doi.org/10.1021/la901298q
D. Das, S.K. Gupta, K. Sudarshan, Europium luminescence as a structural probe to understand defect evolution in CeO2/Eu3+, M3+ (M = Y and La): contrasting role of codopant ionic size. J. Mater. Sci. 56, 17205–17220 (2021). https://doi.org/10.1007/s10853-021-06366-3
C. Secu, C. Bartha, E. Matei, C. Radu, M. Secu, Structural and optical characterization of silica nanospheres embedded with monodisperse CeO2-Eu3+ nanocrystals. Magnetochemistry 8, 22 (2022). https://doi.org/10.3390/magnetochemistry8020022
R. Zhou, Y. Ren, Q. Lu, N. Mahinpey, Microwave-assisted hydrothermal synthesis of Ru/Ceo2 catalyst for efficient and stable low-temperature dry reforming of methane. SSRN J. (2022). https://doi.org/10.2139/ssrn.4189620
A.E. D’Achille, Morphology-dependent fluorescence of europium-doped cerium oxide nanomaterials. Nanoscale Adv. 3, 3563–3572 (2021). https://doi.org/10.1039/D1NA00096A
W.Y. Hernández, O.H. Laguna, M.A. Centeno, J.A. Odriozola, Structural and catalytic properties of lanthanide (La, Eu, Gd) doped ceria. J. Solid State Chem. 184(11), 3014–3020 (2011). https://doi.org/10.1016/j.jssc.2011.09.018. (ISSN 0022-4596)
S.K. Samdarshi, A.K. Agrawal, S. Chauhan et al., Oxygen vacancies induce changes in lattice parameter, photoluminescence characteristics and Raman spectra of sol–gel derived fluorite-type cubic CeO2 and Ce0.8ZrO2−xAxO2 (A = Co/Fe, x = 0–02) powders. Appl. Phys. A 128, 712 (2022). https://doi.org/10.1007/s00339-022-05860-y
K. Zamani, N. Allah-Bakhshi, F. Akhavan et al., Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa. BMC Biotechnol. 21, 68 (2021). https://doi.org/10.1186/s12896-021-00727-1
K. Vignesh, D. Sivaganesh, S. Saravanakumar, M. Prema Rani, Ho3+ induced ZnO: structural, electron density distribution and antibacterial activity for biomedical application. Appl. Biochem. Biotechnol. (2022). https://doi.org/10.1007/s12010-022-03865-0
A.L. Patterson, The Scherrer formula for X-ray particle size determination. Phys. Rev. 56(10), 978 (1939)
W.M. Mohammed, T.H. Mubark, R.M.S. Al-Haddad, Effect of CuO Nanoparticles on Antimicrobial Activity Prepared by Sol-Gel Method. Int. J. Appl. Eng. Res. 13(12), 10559–10562 (2018) © Research India Publications. http://www.ripublication.com(ISSN 0973-4562)
S. Mahalakshmi, V. Vishnu, K. Jamuna, G. Kurian Physiochemical investigation and biovaluation of TiO2 nanocrystals synthesized by chemical and green route. Int. J. Pharm. Pharm. Sci. 6(11) 396-400 (2014)
M.S. Hassan, T. Amna, O.-B. Yang, M.H. El-Newehy, S.S. Al-Deyab, M.-S. Khil, Smart copper oxide nanocrystals: synthesis, characterization, electrochemical and potent antibacterial activity. Colloids Surf. B Biointerfaces 97, 201–206 (2012). https://doi.org/10.1016/j.colsurfb.2012.04.032
K. Vignesh, D. Sivaganesh, S. Saravanakumar, M. PremaRani, Synthesis and characterisation of yittrium doped cerium oxide nanoparticles and their efficient antibacterial application invitro against gram-positive and gram-negative pathogens. J. Mater. Today Proc. (2022). https://doi.org/10.1016/j.matpr.2022.05.178
V. Petrícek, M. Dušek, L. Palatinus, Crystallographic computing system JANA2006: general features. Zeitschrift fur Krist. 229(5), 345 (2014)
W.S. Rasband, ImageJ (U. S. National Institutes of Health, Bethesda, Maryland, USA, 1997–2018). https://imagej.nih.gov/ij/ February 29, 2016
G. Killivalavan, B. Sathyaseelan, G. Kavitha, I. Baskarann, K. Senthilnathan, D. Sivakumar, N. Karthikeyan, E. Manikandan, M. Maaza, Cobalt metal ion doped cerium oxide (Co-CeO2) nanoparticles effect enhanced photocatalytic activity. MRS Adv. (2020). https://doi.org/10.1557/adv.2020.296
A.R. Abhijith, A.K. Srivastava, A. Srivastava, Synthesis and characterization of magnesium doped ZnO using chemical route. J. Phys. Conf. Ser. 1531, 012005 (2020). https://doi.org/10.1088/1742-6596/1531/1/012005
G. Xiong, U. Pal, J.G. Serrano, K.B. Ucer, R.T. Williams, Photoluminesence and FTIR study of ZnO nanoparticles: the impurity and defect perspective. Phys. Status Solidi (c) 3, 3577–3581 (2006). https://doi.org/10.1002/pssc.200672164
N. Shanmugam et al., Luminance behavior of Ce3+ doped ZnS nanostructures. Spectrochim. Acta Part A 118, 557–563 (2014)
B. Abebe, E.A. Zereffa, A. Tadesse et al., A review on enhancing the antibacterial activity of ZnO: mechanisms and microscopic investigation. Nanoscale Res. Lett. 15, 190 (2020). https://doi.org/10.1186/s11671-020-03418-6
T.U.D. Thi, T.T. Nguyen et al., Green synthesis of ZnO nanoparticles using orange fruit peel extract for antibacterial activities. RSC Adv. (2020). https://doi.org/10.1039/D0RA04926C
C.R. Mendes, G. Dilarri, C.F. Forsan et al., Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens. Sci. Rep. 12, 2658 (2022). https://doi.org/10.1038/s41598-022-06657-y
S. MoniriJavadhesari, S. Alipour, S. Mohammadnejad, M.R. Akbarpour, Antibacterial activity of ultra-small copper oxide(II) nanoparticles synthesized by mechanochemical processing against S. aureus and E. coli. Mater. Sci. Eng. C (2019). https://doi.org/10.1016/j.msec.2019.110011
K.S. Khashan, G.M. Sulaiman, F.A. Abdulameer, S. Albukhaty, M.A. Ibrahem, T. Al-Muhimeed, A.A. AlObaid, Antibacterial activity of TiO2 nanoparticles prepared by one-step laser ablation in liquid. Appl. Sci. 11(10), 4623 (2021). https://doi.org/10.3390/app11104623
H.T. Bui, S. Weon, J.W. Bae, E.-J. Kim, B. Kim, Y.-Y. Ahn, W. Kim, Oxygen vacancy engineering of cerium oxide for the selective photocatalytic oxidation of aromatic pollutants. J. Hazard. Mater. (2020). https://doi.org/10.1016/j.jhazmat.2020.123976
J. Xu, C. Zhang, Oxygen vacancy engineering on cerium oxide nanowires for room-temperature linalool detection in rice aging. J Adv Ceram 11, 1559–1570 (2022). https://doi.org/10.1007/s40145-022-0629-8
A. Kumar, S. Babu, A.S. Karakoti, A. Schulte, S. Seal, Luminescence properties of europium-doped cerium oxide nanoparticles: role of vacancy and oxidation states. Langmuir 25(18), 10998–11007 (2009). https://doi.org/10.1021/la901298q
Langmuir. 25(18), 10998–11007, (2009). https://doi.org/10.1021/la901298q
Y.-H. Peng, C.-C. He, Y.-J. Zhao, X.-B. Yang, J. Appl. Phys. 133(7), 075702 (2023). https://doi.org/10.1063/5.0135162
R. Li, L. Liu, B. Ming, Y. Ji, R. Wang, Oxygen vacancy effect on photoluminescence of KNb3O8 nanosheets. Appl. Surf. Sci. (2018). https://doi.org/10.1016/j.apsusc.2017.12.218
J.W. Kim, N.S. Covel, P.C. Guess, E.D. Rekow, Y. Zhang, J. Dent. Res. 89, 91–95 (2010)
F. Izumi, R.A. Dilanian, Recent Research Developments in Physics Part II, vol. 3 (Transworld Research Network, Trivandrum, 2002)
K. Momma, T. Ikeda, A.A. Belik and F. Izumi, "Dysnomia, a computer program for maximum-entropy method (MEM) analysis and its performance in the MEM-based pattern fitting." Powder Diffraction, 28, 184-193 (2013).
K. Momma, F. Izumi, VESTA 3 for three-dimensional visualization of crystal, volumetric and morphology data. J. Appl. Crystallogr. 44, 1272 (2011)
G. Leelaprakash, S. Mohan, Invitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int. J. Drug Dev. Res. 3(3), 189–196 (2011)
Acknowledgements
The Madura College in Madurai, Tamil Nadu, and Kalasalingam Academy of Research and Education (KARE), which provided the PXRD, SEM, and EDAX analyses, are acknowledged by the authors for their support of the research. One of the authors, D. Sivaganesh thanks the Ministry of Science and Higher Education of the Russian Federation for support (Ural Federal University Program of Development within the Priority-2030 Program, project. 4.38).
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Writing—preparation of the initial draft: [KV]; resources: [KV]; writing—review and editing: [DS], supervision: [MPR]; conceptualization: [KV]; methodology: [KV]; formal analysis and investigation: [KV]; writing—original draft preparation: [KV]: review question: [VK].
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Vignesh, K., Sivaganesh, D., Kavitha, V. et al. Unveiling the luminescent brilliance of europium-sourced cerium oxide: a comprehensive exploration for biomedical advancements through in vitro and in vivo studies. Appl. Phys. A 129, 703 (2023). https://doi.org/10.1007/s00339-023-06957-8
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DOI: https://doi.org/10.1007/s00339-023-06957-8