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Multiwalled carbon-aptamer conjugates for dielectric detection of matrix metalloproteinase-9

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Abstract

Abdominal aortic aneurysm (AAA) is a bulge in the abdominal aorta and cause high mortality and morbidity due to the unbalanced condition with haemostasis. AAA has been found with the diameter of 5.5 cm/lager needs a surgical treatment; otherwise, it ruptures with the excessive bleeding. Aneurysm infiltrating macrophage is located in the site of damaged tissue and actively expresses matrix metalloproteinase-9 (MMP-9). MMP-9 is highly correlated with increasing diameter of aneurysm. This research work was designed to detect MMP-9 level on interdigitated electrode (IDE) sensing system to identify the condition of AAA. Specifically, selected aptamer against MMP-9 was utilized as the probe to detect MMP-9 on IDE. MMP-9 detection was enhanced by modifying the surface of IDE with multiwalled carbon nanotube conjugated aptamer through the amine linker. On the linear range between 1 and 60 pM, the limit of detection was at 2 pM with the linear regression (y = 0.2579x + 0.0246; R2 = 0.9477). In addition, spiking of MMP-9 in human serum did not interfere the detection with the IDE designed.

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Acknowledgment

S.C.B.G. thanks Universiti Malaysia Perlis for providing a special grant (9001-00596) to assist with high-impact publication.

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Authors and Affiliations

  1. Department of Cardiothoracic, Xi’an Fourth Hospital, No. 21 Jiefang Road, Xincheng District, Xi’an, 710004, Shaanxi, China

    Jianqiang Guo

  2. Department of Anesthesiology, Shanghai Fourth People’s Hospital Affiliated To Tonji University, Shanghai, 200081, China

    Jingjing Tan

  3. Department of General Surgery, Shanghai Fourth People’s Hospital Affiliated To Tongji University, Shanghai, 200081, China

    Ning Dou

  4. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia

    Thangavel Lakshmipriya & Subash C. B. Gopinath

  5. Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia

    Subash C. B. Gopinath

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  1. Jianqiang Guo
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  2. Jingjing Tan
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  5. Subash C. B. Gopinath
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Correspondence to Subash C. B. Gopinath.

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Guo, J., Tan, J., Dou, N. et al. Multiwalled carbon-aptamer conjugates for dielectric detection of matrix metalloproteinase-9. Appl. Phys. A 127, 95 (2021). https://doi.org/10.1007/s00339-021-04278-2

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  • DOI: https://doi.org/10.1007/s00339-021-04278-2

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