Understanding how rising seawater pCO2 and temperatures impact coral aragonite accretion is essential for predicting the future of reef ecosystems. Here, we report 2 long-term (10–11 month) studies assessing the effects of temperature (25 and 28 °C) and both high and low seawater pCO2 (180–750 μatm) on the calcification, photosynthesis and respiration of individual massive Porites spp. genotypes. Calcification rates were highly variable between genotypes, but high seawater pCO2 reduced calcification significantly in 4 of 7 genotypes cultured at 25 °C but in only 1 of 4 genotypes cultured at 28 °C. Increasing seawater temperature enhanced calcification in almost all corals, but the magnitude of this effect was seawater pCO2 dependent. The 3 °C temperature increase enhanced calcification rate on average by 3% at 180 μatm, by 35% at 260 μatm and by > 300% at 750 μatm. The rate increase at high seawater pCO2 exceeds that observed in inorganic aragonites. Responses of gross/net photosynthesis and respiration to temperature and seawater pCO2 varied between genotypes, but rates of all these processes were reduced at the higher seawater temperature. Increases in seawater temperature, below the thermal stress threshold, may mitigate against ocean acidification in this coral genus, but this moderation is not mediated by an increase in net photosynthesis. The response of coral calcification to temperature cannot be explained by symbiont productivity or by thermodynamic and kinetic influences on aragonite formation.
Coral reefs are among the world’s most biologically diverse ecosystems and are of substantial economic importance in terms of fisheries, tourism and coastal protection (Hoegh-Guldberg et al. 2007). Rising atmospheric CO2 is causing ocean warming (IPCC 2013) and has fundamentally affected seawater carbonate chemistry, lowering seawater pH (Caldeira and Wickett 2003). Understanding the impact of these changes on the accretion of coral aragonite is essential for predicting the future of reef ecosystems. However, attempts to assess increasing pCO2 and temperature effects, either separately or in combination, have generated contradictory reports (Gattuso and Riibesell 2011; Erez et al. 2011). Most laboratory and field studies indicate that calcification is reduced at lower seawater pH, but there is considerable disagreement on the magnitude of this effect both within and between coral species (Erez et al. 2011). The effect of increasing seawater pCO2 and temperature on calcification also varies widely among coral taxa (e.g. Schoepf et al. 2013; Anthony et al. 2008). Unravelling the source of these discrepancies is key to predicting accurately the effects of increasing seawater pCO2 and temperature in different coral species and reef environments.
Interpretation of seawater pCO2 and temperature effects is complicated by dissimilarities in experimental design between studies. Coral responses can be modified by variations in other environmental parameters, e.g. light and nutrient availability (Chan and Connolly 2013; Chauvin et al. 2011), by study duration (Huang et al. 2014; Castillo et al. 2014) or by nonlinear relationships between seawater pCO2/temperature and calcification (e.g. Anthony et al. 2008; Castillo et al. 2014). Many combined seawater pCO2/temperature studies raise temperature above the coral thermal stress threshold (Castillo et al. 2014; Bahr et al. 2016). These studies mimic the effects of stressful, short-term increases in seawater temperature but do not simulate the impact of more subtle, long-term temperature change (Hoegh-Guldberg et al. 2007).
Relatively few studies explore the interactions of coral photosynthesis and respiration with calcification under increased seawater pCO2/temperature, despite the potential for algal symbionts to offset ocean acidification effects. Increasing seawater pCO2 can fertilise photosynthesis (Mackey et al. 2015) and this may ameliorate cellular acidosis (Gibbin and Davy 2014), as CO2 consumption increases local fluid pH when HCO3− and H+ react to form CO2 and H2O. In support of this hypothesis, the intracellular pH of symbiotic coral cells is higher in the light than in dark (Venn et al. 2009) and symbiont activities counteract the decrease in intracellular pH of host coral cells which are exposed to high pCO2 (Gibbin et al. 2014). Photosynthesis may also provide an energy source as algal photosynthate is translocated to the coral (Muscatine and Cernichiari 1969). The responses of coral photosynthesis and respiration to elevated seawater pCO2 are inconsistent (reviewed in Noonan and Fabricius 2016). Increasing seawater pCO2 may stimulate (Langdon and Atkinson 2005; Crawley et al. 2010; Gibbin and Davy 2014; Noonan and Fabricius 2016) or suppress (Anthony et al. 2008; Edmunds 2012) photosynthesis. Symbiont photosynthesis may be limited by light or nutrients (rather than carbon) in the culture seawater (Chauvin et al. 2011) or may be affected by symbiont type. The growth and photosynthetic response of Symbiodinium to elevated pCO2 is type specific in culture (Brading et al. 2011) and in hospite (Graham and Sanders 2016).
Here, we present a long-term (17–22 week acclimation) study designed to test the effect of increasing seawater pCO2 (from 180 to 750 μatm) and temperature (25 and 28 °C) on massive Porites spp. corals. Massive poritids are major components of coral reefs in the Indo-Pacific, are found in almost all reef habitats and can be the most important reef framework builders (Pichon 2011). Responses of coral colonies to temperature and seawater pCO2 are highly variable for a range of coral species (Kavousi et al. 2015; Shaw et al. 2016), and we measured the individual responses of colonies of a range of genotypes. We utilised seawater pCO2 which were both lower and higher than the present day, and we measured the effect of environmental change on colony calcification, photosynthesis and respiration to explore how these physiological processes interact.
We tested the impact of variations in seawater pCO2 and temperature on calcification, respiration and photosynthesis of massive Porites spp. corals in 2 experiments. In each experiment, corals were acclimated to altered seawater pCO2 for at least 17 weeks before study. We cultured 3 different genotypes in experiment 1 and 4 different genotypes in experiment 2. All corals were harvested in Fiji by a collector and imported into the UK. We defined different genotypes as coral heads collected from large, spatially separate (non-adjoining) colonies. We cut multiple colonies (each ~ 12 cm in diameter) from each head to enable the study of large individuals of the same genotype in each treatment. Small experimental colonies do not provide accurate estimates of the physiological performance of larger colonies, even when area-normalised (Edmunds and Burgess 2016). In both experiments, corals were cultured at seawater pCO2 of ~ 180 µatm (the CO2 atmosphere during the last glacial maximum, Petit et al. 1999), ~ 400 µatm (the present day) and ~ 750 µatm (projected to occur by the end of the present century, IPCC 2013). In experiment 2, corals were also cultured at ~ 260 µatm (the CO2 atmosphere during the pre-industrial, Gattuso and Lavigne 2009). In experiment 1, corals were maintained at 25 °C and this demonstrated that photosynthesis and respiration rates could vary significantly between replicate colonies of the same genotype. In experiment 2, the same coral colonies were cultured at both 25 and 28 °C for separate periods to enable us to measure the performance of each individual as a function of temperature. Our upper experimental temperature is close to the maximum seasonal temperature at the coral source site (Fiji, where seasonal seawater temperatures typically range from 25.5 to 29 °C) and is likely to be below the thermal stress threshold for these specimens. Culture system parameters are summarised in Table 1.
Corals were maintained in a purpose-built large-volume aquarium system constructed of low CO2 permeability materials designed to control temperature, salinity and dissolved inorganic carbon (DIC) system parameters within narrow limits (see Cole et al. 2016). Corals were housed in 21-l cast acrylic tanks, recirculated with seawater from high-density polyethylene reservoirs containing ~ 900 l of seawater. At the start of each experiment, the reservoir seawater was ~ 80–85% fresh artificial seawater (Red Sea Salt, Red Sea Aquatics, UK) diluted with either artificial seawater from a mixed coral/fish aquarium (Experiment 1) or with local seawater (Experiment 2). 10–15 l of seawater was usually removed from each reservoir each week (during removal of microalgae from the tank surfaces) and was replaced with fresh artificial seawater. No seawater replacement occurred during the experimental periods. The reservoirs were bubbled (at ~ 10 l min−1) with gas mixes set to reach the target seawater pCO2 compositions. The 400-µatm treatment was aerated with untreated ambient air drawn from outside the building approximately 150 m from the open coast. The high- and low-CO2 treatments were aerated with ambient or low-CO2 air, respectively, combined with high-purity CO2 (Foodfresh, BOC, UK) to achieve the required gas composition. Low-CO2 air was produced by bubbling ambient air through a caustic solution (0.9 M NaOH and 0.1 M Ca(OH)2) and rinsing it by bubbling through deionised water. The [CO2] of this low-CO2 air was monitored every 2 h by automated non-dispersive infrared CO2 analysers (WMA04, PP systems, USA) and ranged from ~ 20 to 100 µatm depending on the age of the caustic solution. Flow rates of air (ambient or low-CO2) and CO2 were regulated by high-precision mass flow controllers (SmartTrak 50 Series, Sierra USA) controlled by a purpose-written MATLAB® program. The [CO2] of the gas streams (after any addition of CO2) were monitored automatically 3–4 times per day and were held within narrow limits (Table 1). Corals were maintained under LED lighting (Maxspect R420R 160w-10000k) on a 12-h light/12-h dark cycle, with wavelength settings of 100% A and 20% B such that photosynthetically active radiation (PAR) intensity at coral depth was ~ 300 μmol photons m−2 s−1. Corals were fed weekly with rotifers.
Acclimation and study timing
In experiment 1, imported corals were maintained at ambient seawater pCO2 conditions for 2 months, adjusted to pCO2 treatment conditions over another 2 months and then acclimated at the final treatment pCO2 for 5 months before study, all at 25 °C. This was followed by a 5-week experimental period in which calcification, respiration and net and gross photosynthesis were measured in each coral colony on usually 3 or 4 occasions. In experiment 2, imported corals were maintained at ambient seawater pCO2 conditions for 2 months, adjusted to pCO2 treatment conditions over 1 month and then acclimated at the final treatment pCO2 for 4 months at 28 °C. This was followed by a 5-week experimental period in which physiological rates were measured as before. At the end of this time, a small slice of each colony (including tissue and skeleton) was removed by rock saw and preserved for future analysis. This reduced the surface areas of each colony by ~ 25%. Seawater temperatures were then reduced to 25 °C over a period of 4 weeks and then acclimated at this temperature for another month during which time the sawn edges of the coral colonies were overgrown by coral tissue. Finally, physiological rates were measured in each coral over a 5-week period as before. In experiment 2, the coral polyps did not extend in the colonies cultured at 400 μatm and at 28 °C and there was little microalgae growth on the tank surfaces in this treatment. The seawater in this reservoir was removed and replaced with seawater from the remaining 3 reservoirs (bubbled to bring it to the correct seawater pCO2 before use). Physiological measurements were only made in these colonies at 25 °C.
To measure physiological rates, individual coral colonies were isolated in 21-l culture tanks for 5 h (in the light) or 7 h (in the dark). Net photosynthesis and respiration rates were estimated from measurements of dissolved oxygen (DO2, Thermo Orion 5 star meter with RDO sensor) at the start and end of each incubation (Schneider and Erez 2006). Net photosynthesis was defined as the production of oxygen in the light and respiration as the consumption of oxygen in the dark. Gross photosynthesis was calculated as net photosynthesis minus respiration. The precision of repeat DO2 measurements was always better than 0.3% (1σ). [DO2] typically increased by up to ~ 80 μmol l−1 (from ~ 200 μmol l−1) in the light and decreased by up to ~ 40 μmol l−1 in the dark. Calcification rates were estimated from the change in seawater total alkalinity (typically ~ 50 μeq kg−1) over the course of the isolation (Schneider and Erez 2006). Light and dark calcification rates were combined to yield a total rate per day. Isolations were repeated 3–4 times (with the exception of 2 coral colonies which were only isolated twice) at approximately weekly intervals over the 4-week study period. All metabolic rates were normalised to coral surface area. Coral tissue surface areas were estimated by multiplying the tissue area measured on scaled photographs using image processing software (ImageJ, National Institute of Health, USA) by a hummockiness factor to correct for colony topography. The hummockiness factor was calculated for each colony by dividing the actual length of the colony surface (measured with a piece of thread) by the length of the colony surface estimated from ImageJ.
Monitoring seawater composition
Seawater temperatures were measured hourly (TinyTag Aquatic, Gemini Data Loggers, UK) in experiment 1. Temperatures in experiment 2 and salinities in both experiments were measured twice daily on 4 days of each week in the experimental periods (Thermo Orion 5 star meter, calibrated to NIST conductivity standards). Total alkalinity was measured by automated Gran titration (Metrohm, 888 Titrando) twice daily on 4 days of each week in the experimental periods. Precision of duplicate ~ 30 ml analyses was typically ± 2 μeq kg−1. The precision of multiple measurements of synthetic Na2CO3 standards was consistently ± 3 μeq kg−1 (1σ, between days). The total alkalinity, [Ca] and [Sr] of the culture seawater were maintained by additions of 0.6 M Na2CO3 and a mixture of 0.58 M CaCl2 + 0.02 M SrCl2 by 200 μl volume solenoid diaphragm pumps, evenly spaced over a 24-h period, controlled by a custom-written MATLAB® dosing control program. DIC was measured weekly in each reservoir using a CO2 differential, non-dispersive, infrared gas analyser (Apollo SciTech; AS-C3). Samples were calibrated against a natural seawater certified reference material (CRM; A. Dickson, Scripps Institution of Oceanography). Internal reproducibility was calculated from the standard deviation of 8 replicate measurements of a single sample (σ/√n) and was always ≤ 0.1%. Multiple measurements of the CRM analysed as an unknown were in excellent agreement with the certified value (within 5 μmol kg−1, Cole et al. 2016).
Calcification, gross and net photosynthesis and respiration rates for all corals are illustrated in Figs. 1 and 2. Two replicate colonies of genotype 3 were cultured and measured in 400 and 750 μatm seawater pCO2 (Fig. 1). Calcification rates, normalised to coral surface area, did not vary significantly between replicates, but both gross photosynthesis and respiration rates did vary significantly (p ≤ 0.05 two-tailed t test). Light variations over the tank footprint were small (< 50 μmol photons m−2 s−1), but photosynthesis variations between replicates may reflect individual topography or modulation of light scattering (Marcelino et al. 2013). We maintained each colony in the same tank position for the duration of each experiment ensuring that measurements of the same colony in different temperatures are directly comparable.
Physiological rates, particularly calcification, were highly variable between genotypes. For example, genotype 7 maintained calcification rates that were ≥ ×2 higher than genotypes 5 and 6 regardless of seawater pCO2 or temperature (Fig. 2). To ease comparison of the effect of seawater pCO2 on physiological processes, we calculated the relative calcification, respiration, gross and net photosynthesis rates of each coral genotype as a proportion of the rate observed at the lowest seawater pCO2 (180 μatm, Fig. 3). We compared physiological rates between individuals of the same genotype in different seawater pCO2 treatments (one-way ANOVA, Tables 2, 3). We combined data from the 2 replicate genotype 3 colonies in experiment 1 for these analyses.
At 25 °C, calcification was significantly lower at 750 μatm pCO2 compared to 180 μatm pCO2 in 4 of the 7 Porites spp. genotypes and significantly lower at 750 μatm pCO2 compared to 400 μatm pCO2 in 1 genotype (Tables 2, 3). At 28 °C, calcification was significantly lower in only one of the 4 genotypes cultured at 750 μatm pCO2 compared to 180 μatm pCO2 (and was significantly higher in 1 genotype). Gross and net photosynthesis and respiration rates were significantly lower at 750 μatm pCO2 than at 180 μatm pCO2 in 2 of the 7 genotypes cultured at 25 °C. At 28 °C, the pattern was more complicated with some genotypes exhibiting significantly higher photosynthesis or respiration rates at high or low seawater pCO2 (Table 3).
To assess the effect of temperature changes, we compared physiological rates at 25 and 28 °C for each coral colony (two-tailed t test, Table 4). To avoid inter-colony differences (such as those observed in experiment 1) affecting the results, we measured the responses of the same individual colonies to seawater pCO2 first at 28 °C and then 3 months later at 25 °C. We cannot discount the possibility that physiological rates were affected by the time that individual corals spent in the culture system, but we believe that any effect is likely to be small. Individual Porites spp. colonies which we have cultured in our aquaria for > 4 years at constant temperature exhibit similar calcification rates (unpublished data) to those observed for massive Porites spp. in the field (Allison et al. 1996), indicating that corals maintain approximately constant calcification rates in the aquaria. Increasing seawater temperature usually increased calcification but decreased respiration and gross and net photosynthesis rates (Fig. 2). Increasing temperature significantly increased calcification in three of the 4 genotypes at 750 μatm pCO2 but did not significantly affect calcification at 180 or 260 μatm (Table 4). Increasing temperature significantly decreased gross and net photosynthesis in only one genotype at 750 μatm pCO2 but in all genotypes at 180 μatm.
Increasing seawater pCO2 decreased calcification significantly in 4 of 7 massive Porites spp. genotypes at 25 °C but in only 1 of 4 genotypes at 28 °C. Our results are broadly comparable to 2 previous shorter (11 days and 8 weeks) studies of massive Porites spp. in which calcification rates were reduced by high seawater pCO2 at 25–26 °C (Anthony et al. 2008) but were not significantly affected at 28–29 °C (Anthony et al. 2008; Edmunds 2012). While the majority of studies indicate that coral calcification is reduced by ocean acidification (Erez et al. 2011), massive Porites spp. appear to be relatively insensitive to increasing seawater pCO2 (Crook et al. 2012) and have higher rates of calcification at some natural high seawater pCO2 sites compared to their counterparts at adjacent low-pCO2 sites (Fabricius et al. 2011).
Increasing seawater temperature enhanced calcification in almost all corals, and the magnitude of this effect was seawater pCO2 dependent. The 3 °C temperature increase enhanced calcification on average by 3% at 180 μatm, by 35% at 260 μatm and by > 300% at 750 μatm. At high seawater pCO2, this increase substantially mitigated the effects of ocean acidification on calcification: at 25 °C the mean Porites spp. calcification rate at seawater pCO2 750 μatm is ~ 40% of the rate observed at 180 μatm. In contrast, at 28 °C this figure is ~ 100%. The temperature driven increases in calcification were not accompanied by increases in symbiont gross or net productivity (Fig. 2). Rather, net production was reduced at the higher temperature in all colonies regardless of seawater pCO2 (Fig. 2). Our results concur with a previous massive Porites spp. study in which net productivity was reduced at high seawater temperature and seawater pCO2, but calcification was not affected (Anthony et al. 2008). Our study indicates that increasing seawater temperature can offset the suppression of calcification by ocean acidification in this coral species. However, this offset is not associated with an increase in net algal productivity.
To understand how increasing temperature may offset ocean acidification effects requires some understanding of the coral calcification process. The aragonitic coral skeleton precipitates from a calcifying fluid, derived from seawater (Tambutte et al. 2012) and enclosed in a semi-isolated extracellular space between the coral tissue and underlying skeleton (Clode and Marshall 2002). Corals increase the pH of the calcification fluid above that of seawater (Al-Horani et al. 2003; Venn et al. 2011) shifting the fluid DIC equilibrium in favour of CO32− at the expense of CO2. The CO2 concentration gradient thus formed facilitates the diffusion of CO2 from the overlying coral tissue into the calcification fluid (Erez 1978) where it reacts to form HCO3− and CO32−. It is unclear which DIC species is/are involved in aragonite precipitation. CO32− is usually preserved in the aragonite lattice, but HCO3− could also be involved in precipitation. Wolthers et al. (2012) successfully modelled observed growth rates of the CaCO3 polymorph calcite by assuming both aqueous HCO3− and CO32− attach to the growing crystal. HCO3− subsequently deprotonates, leaving CO32− preserved in the carbonate lattice. Von Euw et al. (2017) observed HCO3− in both coral and synthetic aragonite which may be a remnant of attached un-deprotonated HCO3−. Aragonite precipitation rates correlate positively with fluid aragonite saturation state (Burton and Walter 1987), a function of [Ca2+] and [CO32−], suggesting that CO32− is critical in precipitation. However, similar positive relationships are also observed between aragonite precipitation rate and fluid [HCO3−] or [HCO3− + CO32−] (see supplementary information), so this dataset does not demonstrate sole involvement of CO32− in precipitation. Regardless of the DIC species involved in aragonite formation, the rise in calcification fluid pH and subsequent CO2 invasion increases the availability of both HCO3− and/or CO32− at the calcification site.
The precipitation rates of synthetic aragonites are positively correlated with temperature and fluid saturation state (Fig. 4, replotted from Burton and Walter 1987). We overlay our observed increases in coral calcification rate as a function of temperature onto Fig. 4 assuming that the calcification fluid aragonite saturation states (Ω) of all corals growing at 25 °C are ~ 8. Coral calcification fluid [Ca2+] is similar to seawater (Al-Horani et al. 2003), suggesting that [precipitating DIC] drives calcification rate. The mean calcification site pHtotal of modern massive Porites spp. field corals growing at ambient pCO2 and a mean annual seawater temperature of 25 °C is ~ 8.5 (Allison et al. 2014). In paired laboratory measurements of both calcification fluid pH and [CO32−] in a range of coral species, a coral calcification fluid of pHtotal 8.5 has an aragonite saturation state of ~ 8 (Cai et al. 2016). Corals cultured at high seawater pCO2 are unable to attain the same high calcification fluid pH observed in their low seawater pCO2 counterparts (Venn et al. 2012), perhaps because low seawater pH may inhibit the dissipation of H+ from the coral to local seawater (Jokiel 2011). It is therefore unlikely that they concentrate DIC at the calcification site to the same extent or reach the same high fluid saturation states. Decreasing Ωaragonite to 6 reduces aragonite precipitation rate by about half, while raising it to 10 (as we hypothesise may occur at low seawater pCO2) increases precipitation by ~ 50%. While our selection of calcification fluid saturation states at high and low seawater pCO2 is arbitrary, it serves to illustrate a key point. Inorganic aragonite precipitation rates are temperature dependent, increasing × 1.6 from 25 to 28 °C at Ωaragonite = 8, but saturation state has little effect on this temperature dependence (i.e. this figure changes from 1.5 to 1.7 at Ωaragonite = 6 and 10, respectively, Fig. 4). In our study, temperature had little effect on coral calcification at low seawater pCO2 and any observed increase in aragonite precipitation is much lower than that observed in inorganically precipitated aragonites. In contrast at high seawater pCO2, the temperature-mediated increase in calcification is much larger (× 3) than observed in inorganic aragonites. Clearly, the response of coral calcification to temperature is not driven solely by thermodynamic and kinetic influences on aragonite precipitation. Rather other factors act to negate and augment the effect of temperature on aragonite precipitation at low and high seawater pCO2, respectively. While it is not immediately clear what these are, calcification responses of the magnitude observed here can potentially be explained by changes in the saturation state of the calcification fluid (Fig. 4). The fluid saturation state is influenced by enzyme activities. For example, Ca-ATPase extrudes protons from the calcification fluid (Al-Horani et al. 2003), while carbonic anhydrases catalyse the interconversion of CO2 and HCO3− (Zoccola et al. 2016), and these activities may be both temperature and seawater pCO2 dependent. For example, ocean acidification suppresses the gene expression and enzyme activities of carbonic anhydrases localised to the coral calcifying cells but increasing temperatures enhance these enzyme activities and may counteract the effect of seawater pCO2 (Zoccola et al. 2016).
Our data indicate that temperature increases can mitigate against the effect of ocean acidification on calcification in massive Porites spp. It is possible that future seawater temperature increases may offset the effects of ocean acidification in this coral genus at reef locations where corals live below their upper thermal stress thresholds. However, in practice, seawater temperature increases are unlikely to enhance calcification at most tropical reef locations where corals already exist close to their upper thermal tolerance limits. Future minor increases in seawater temperature at these sites are likely to induce widespread coral bleaching and thereby suppress coral calcification (Frieler et al. 2013; Hooidonk et al. 2013). Recent decreases in growth rates of massive Porites spp. at some Indo-Pacific reef locations are likely driven by local increases in local seawater temperatures (Cantin and Lough 2014), indicating that this process is already in progress.
Al-Horani FA, Al-Moghrabi SM, de Beer D (2003) The mechanism of calcification and its relation to photosynthesis and respiration in the scleractinian coral Galaxea fascicularis. Marine Biology 142:419–426
Allison N, Cohen I, Finch AA, Erez J, Tudhope AW (2014) Corals concentrate dissolved inorganic carbon to facilitate calcification. Nature Communications 5:5741. https://doi.org/10.1038/ncomms6741
Allison N, Tudhope AW, Fallick AE (1996) Factors influencing the stable carbon and oxygen isotopic composition of Porites lutea coral skeletons from Phuket, South Thailand. Coral Reefs 15:43–57
Anthony KRN, Kline DI, Diaz-Pulido G, Dove S, Hoegh-Guldberg O (2008) Ocean acidification causes bleaching and productivity loss in coral reef builders. Proceedings of the National Academy of Sciences of the USA 105:17442–17446
Bahr KD, Jokiel PL, Rodgers KS (2016) Relative sensitivity of five Hawaiian coral species to high temperature under high-pCO2 conditions. Coral Reefs 35:729–738
Brading P, Warner ME, Davey P, Smith DJ, Achterberg EP, Suggett DJ (2011) Differential effects of ocean acidification on growth and photosynthesis among phylotypes of Symbiodinium (Dinophyceae). Limnology and Oceanography 56:927–938
Burton EA, Walter LM (1987) Relative precipitation rates of aragonite and Mg calcite from seawater: temperature or carbonate ion control. Geology 15:111–114
Cai W-J, Ma Y, Hopkinson BM, Grottoli AG, Warner ME, Ding Q, Hu X, Yuan X, Schoepf V, Xu H, Han C, Melman TF, Hoadley KD, Pettay DT, Matsui Y, Baumann JH, Levas S, Ying Y, Wang Y (2016) Microelectrode characterization of coral daytime interior pH and carbonate chemistry. Nature Communications 7:11144. https://doi.org/10.1038/ncomms11144
Caldeira K, Wickett ME (2003) Anthropogenic carbon and ocean pH. Nature 425:365
Cantin NE, Lough JM (2014) Surviving coral bleaching events: Porites growth anomalies on the Great Barrier Reef. PLOS One. https://doi.org/10.1371/journal.pone.0088720
Castillo KD, Ries JB, Bruno JF, Westfield IT (2014) The reef-building coral Siderastrea siderea exhibits parabolic responses to ocean acidification and warming. Proceedings of the Royal Society of London B: Biological Sciences 281:20141856. https://doi.org/10.1098/rspb.2014.1856
Chan NC, Connolly SR (2013) Sensitivity of coral calcification to ocean acidification: a meta-analysis. Global Change Biology 19:282–290
Chauvin A, Denis V, Cuet P (2011) Is the response of coral calcification to seawater acidification related to nutrient loading? Coral Reefs 30:911. https://doi.org/10.1007/s00338-011-0786-7
Clode PL, Marshall AT (2002) Low temperature FESEM of the calcifying interface of a scleractinian coral. Tissue and Cell 34:187–198
Cole C, Finch AA, Hintz C, Hintz K, Allison N (2016) Understanding cold bias: variable response of skeletal Sr/Ca to seawater pCO2 in acclimated massive Porites corals. Scientific Reports 6:26888. https://doi.org/10.1038/srep26888
Crawley A, Kline DI, Dunn S, Anthony K, Dove S (2010) The effect of ocean acidification on symbiont photorespiration and productivity in Acropora formosa. Global Change Biology 16:851–863
Crook ED, Potts D, Rebolledo-Vieyra M, Hernandez L, Paytan A (2012) Calcifying coral abundance near low-pH springs: implications for future ocean acidification. Coral Reefs 31:239–245
Dickson AG (1990) Standard potential of the reaction: AgCl(s) + 12H2(g) = Ag(s) + HCl(aq) and the standard acidity constant of the ion HSO4 − in synthetic sea water from 273.15 to 318.15K. J Chem Thermodynamics 22:113–127
Dickson AG, Millero FJ (1987) A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep-Sea Research 34:1733–1743
Edmunds PJ (2012) Effect of pCO2 on the growth, respiration, and photophysiology of massive Porites spp. in Moorea, French Polynesia. Marine Biology 159:2149–2160
Edmunds PJ, Burgess SC (2016) Size-dependent physiological responses of the branching coral Pocillopora verrucosa to elevated temperature and pCO2. Journal of Experimental Biology 291:3896–3906
Erez J (1978) Vital effect on the stable-isotope composition seen in foraminifera and coral skeletons. Nature 273:199–202
Erez J, Reynaud S, Silverman J, Schneider K, Allemand D (2011) Coral calcification under ocean acidification and global change. In: Dubinksy Z, Stambler N (eds) Coral reefs: an ecosystem in transition. Springer, New York, pp 151–176
Fabricius KE, Langdon C, Uthicke S, Humphrey C, Noonan S, De’ath G, Okazaki R, Muehllehner N, Glas MS, Lough JM (2011) Losers and winners in coral reefs acclimatized to elevated carbon dioxide concentrations. Nature Climate Change 1:165–169
Frieler K, Meinshausen M, Golly A, Mengel M, Lebek K, Donner SD, Hoegh-Guldberg O (2013) Limiting global warming to 2 °C is unlikely to save most coral reefs. Nature Climate Change 3:165–170
Gattuso JP, Lavigne H (2009) Technical Note: approaches and software tools to investigate the impact of ocean acidification. Biogeosciences 6:2121–2133
Gattuso JP, Riibesell U (2011) Reconciling apparently contradictory observations. In: Field CB, Barros V, Stocker TF, Qin D, Mach KJ, Plattner G-K, Mastrandrea MD, Tignor M, Ebi KL (eds) Workshop report of the Intergovernmental Panel on Climate Change workshop on impacts of ocean acidification on marine biology and ecosystems. IPCC Working Group II Technical Support Unit, Carnegie Institution, Stanford, pp 10–16
Gibbin EM, Davy SK (2014) The photo-physiological response of a model cnidarian-dinoflagellate symbiosis to CO2-induced acidification at the cellular level. Journal of Experimental Marine Biology and Ecology. https://doi.org/10.1016/j.jembe.2014.03.015
Gibbin EM, Putnam HM, Davy SK, Gates RD (2014) Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology 217:1963–1969
Graham ER, Sanders RW (2016) Species-specific photosynthetic responses of symbiotic zoanthids to thermal stress and ocean acidification. Marine Ecology 37:442–458
Hoegh-Guldberg O, Mumby PJ, Hooten AJ, Steneck RS, Greenfield P, Gomez E, Harvell CD, Sale PF, Edwards AJ, Caldeira K, Knowlton N, Eakin CM, Iglesias-Prieto R, Muthiga N, Bradbury RH, Dubi A, Hatziolos ME (2007) Coral reefs under rapid climate change and ocean acidification. Science 318:1737–1742
Hooidonk R, Maynard JA, Manzello D, Planes S (2013) Opposite latitudinal gradients in projected ocean acidification and bleaching impacts on coral reefs. Global Change Biology 20:103–112
Huang H, Yuan X-C, Cai W-J, Zhang C-L, Li X, Liu S (2014) Positive and negative responses of coral calcification to elevated pCO2: case studies of two coral species and the implications of their responses. Marine Ecology Progress Series 502:145–156
IPCC (2013) Climate Change 2013: the physical science basis. Contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change. In: Stocker TF, Qin D, Plattner G-K Tignor M, Allen SK, Boschung J, Nauels A, Xia Y, Bex V, Midgley PM (eds) Cambridge University Press, Cambridge
Jokiel PL (2011) Ocean acidification and control of reef coral calcification by boundary layer limitation of proton flux. Bulletin of Marine Science 87:639–657
Kavousi J, Reimer JD, Tanaka Y, Nakamura T (2015) Colony-specific investigations reveal highly variable responses among individual corals to ocean acidification and warming. Marine Environmental Research 109:9–20
Langdon C, Atkinson MJ (2005) Effect of elevated pCO2 on photosynthesis and calcification of corals and interactions with seasonal change in temperature/irradiance and nutrient enrichment. Journal of Geophysical Research 110:C09S07. https://doi.org/10.1029/2004JC002576
Mackey KRM, Morris JJ, Morel FMM, Kranz SA (2015) Response of photosynthesis to ocean acidification. Oceanography 28:74–91
Marcelino L, Westneat MW, Stoyneva V, Backman V (2013) Modulation of light-enhancement to symbiotic algae by light-scattering in corals and evolutionary trends in bleaching. PLoS ONE 8:e61492. https://doi.org/10.1371/journal.pone.0061492
Mehrbach C, Culberson CH, Hawley JE, Pytkowicx RM (1973) Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnology and Oceanography 18:897–907
Muscatine L, Cernichiari E (1969) Assimilation of photosynthetic products of zooxanthellae by a reef coral. The Biological Bulletin 137:506–523
Noonan SHC, Fabricius KE (2016) Ocean acidification affects productivity but not the severity of thermal bleaching in some tropical corals. ICES Journal of Marine Science 73:715–726
Petit JR, Jouzel J, Raynaud D, Barkov NI, Barnola JM, Basile I, Bender M, Chappellaz J, Davis M, Delaygue G, Delmotte M, Kotlyakov VM, Legrand M, Lipenkov VY, Lorius C, Pépin L, Ritz C, Saltzman E, Stievenard M (1999) Climate and atmospheric history of the past 420,000 years from the Vostok ice core, Antarctica. Nature 399:429–436
Pichon M (2011) Porites. In: Hopley D (ed) Encyclopedia of modern coral reefs: structure, form and process. Springer, Berlin
Pierrot D, Lewis ED, Wallace WR (2006) MS Excel program developed for CO2 system calculations. Oak Ridge National Laboratory, Oak Ridge
Schneider K, Erez J (2006) The effect of carbonate chemistry on calcification and photosynthesis in the hermatypic coral Acropora eurystoma. Limnology and Oceanography 51:1284–1293
Schoepf V, Grottoli AG, Warner ME, Cai WJ, Melman TF, Hoadley KD, Pettay DT, Hu X, Li Q, Xu H, Wang Y, Matsui Y, Baumann JH (2013) Coral energy reserves and calcification in a high-CO2 world at two temperatures. PLoSone. https://doi.org/10.1371/journal.pone.0075049
Shaw EC, Carpenter RC, Lantz CA, Edmunds PJ (2016) Intraspecific variability in the response to ocean warming and acidification in the scleractinian coral Acropora pulchra. Marine Biology 163:210. https://doi.org/10.1007/s00227-016-298
Tambutte E, Tambutté S, Segonds N, Zoccola D, Venn A, Erez J, Allemand D (2012) Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification. Proceedings of the Royal Society B: Biological Sciences 279:19–27
Uppstrom LR (1974) The boron/chlorinity ratio of the deep-sea water from the Pacific Ocean. Deep-Sea Research 21:161–162
Venn AA, Tambutte E, Holcomb M, Allemand D, Tambutte S (2011) Live tissue imaging shows reef corals elevate pH under their calcifying tissue relative to seawater. PLoS ONE 6:e20013. https://doi.org/10.1371/journal.pone.0020013
Venn AA, Tambutte E, Holcomb M, Tambutte S (2012) Impact of seawater acidification on pH at the tissue-skeleton interface and calcification in reef corals. Proceedings of the National Academy of Sciences of the USA. https://doi.org/10.1073/pnas.1216153110
Venn AA, Tambutté E, Lotto S, Zoccola D, Allemand D, Tambutté S (2009) Imaging intracellular pH in a reef coral and symbiotic anemone. Proceedings of the National Academy of Sciences of the USA 106:16574–16579
Von Euw S, Zhang Q, Manichev V, Murali N, Gross J, Feldman LC, Gustafsson T, Flach C, Mendelsohn R, Falkowski PG (2017) Biological control of aragonite formation in stony corals. Science 356:933–938
Wolthers M, Nehrke G, Gustafsson JP, Van Cappellen P (2012) Calcite growth kinetics: modelling the effect of solution stoichiometry. Geochimica et Cosmochimica Acta 77:121–134
Zoccola D, Innocenti A, Bertucci A, Tambutté E, Supuran CT, Tambutté S (2016) Coral carbonic anhydrases: regulation by ocean acidification. Marine Drugs 14:109. https://doi.org/10.3390/md14060109
This work was supported by the UK Natural Environment Research Council (Award NE/I022973/1) to AAF and NA. We thank Dave Steven, Mark Robertson, Casey Perry, Mike Scaboo and Andy Mackie for their assistance with the culture system build.
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On behalf of all authors, the corresponding author states that there is no conflict of interest.
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Cole, C., Finch, A.A., Hintz, C. et al. Effects of seawater pCO2 and temperature on calcification and productivity in the coral genus Porites spp.: an exploration of potential interaction mechanisms. Coral Reefs 37, 471–481 (2018). https://doi.org/10.1007/s00338-018-1672-3
- Ocean acidification
- Seawater temperature