Abstract
A part of mouse Zfy-2 sequence was synthesized and used to screen a genomic library of the spinous country-rat (Tokudaia osimensis spp., 2n = 45). An isolated clone had the C-terminal region of Zfy, which consisted of 1190 bp, encoded 336 amino acid residues, and harbored 11 out of 13 zinc finger motifs. With this as a probe, a bovine testis cDNA library was screened. Two ZFX clones were isolated and their sequences combined. The short sequence, lacking part of the 5′ upstream region, was amplified by PCR or RT-PCR, cloned, and sequenced. A full-length ZFX was constructed by combining these three sequences. The bovine ZFX consisted of 5328 bp and encoded 800 amino acid residues, which contained 13 zinc finger motifs. ZFX was used as a probe for fluorescence in situ hybridization and was mapped to Xq34, different from its previously reported site at Xq21–q231. A SINE (short interspersed nuclear element) sequence consisting of 188 bp was found close to the end of the 3′-untranslated region of ZFX. The SINE sequence hybridized to all bovine chromosomes. ZFY is highly homologous with ZFX and, as a result, ZFY could be mapped simultaneously. ZFY was mapped to the distal region of the short arm of the Y Chromosome (Chr) (Yp13), contradicting the previously reported position Yq1. Ovine and caprine ZFY were also mapped with bovine ZFX. Both were mapped to the distal region of the short arm of the Y Chr (Yp12–p13). Ovine ZFX was mapped to a region close to the centromere of the X Chr (Xq13).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agulnik AI, Bishop CE, Lerner JL, Agulnik SI, Solovyev VV (1997) Analysis of mutation rates in the SMCY/SMCX genes shows that mammalian evolution is male driven. Mamm Genome 8, 134–138
Cui X, Kato Y, Sato S, Sutou S (1995) Mapping of bovine SRY gene to the distal tip of long arm and murine Sry to the distal tip of the short arm of the Y-chromosome. Anim Sci Technol (Jpn) 66, 441–444
Cui X, Kato Y, Sato S, Sutou S (1996a) Mapping of the sheep and goat Sry genes to the long arms of the Y-chromosomes by the FISH method. Anim Sci Technol (Jpn) 67, 333–337
Cui X, Kudo T, Sutou S (1996b) Sexing of bovine embryos with malespecific repetitive DNA by polymerase chain reaction: characterization and mapping of bovine male-specific and gender-neutral repetitive DNA. J Reprod Dev 42, 125–131
DiBerardino D, Hayes H, Fries R, Long S (1989) International system for cytogenetic nomenclature of domestic animals (1989) Cytogenet Cell Genet 53, 65–79
Honda K, Suzuki H, Itoh M (1977) An unusual sex chromosome constitution found in the Amami spinous country-rat, Tokudaia osimensis osimensis. Jpn J Genet 52, 247–249
Honda K, Suzuki H, Itoh M, Hayashi K (1978) Karotypical differences of the Amami spinous country-rats, Tokudaia osimensis osimensis obtained from two neighboring islands. Jpn J Genet 53, 297–299
Iannuzzi L, Di Meo GP, Perucatti A (1994) An improved characterization of goat chromosome by means of G- and R-band comparison. Hereditas 120, 245–251
Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1995) G- and R-banding comparison of sheep (Ovis aries L.) chromosomes. Cytogenet Cell Genet 68, 85–90
Lenstra JA, van Boxtel JA, Zwaagstra KA, Schwerin M (1993) Short interspersed nuclear elements (SINE) sequence of the Bovidae. Anim Genet 24, 33–39
Loginova UA, Derusheva CE, Chiryaeva OG, Bosak NP, Efimov AM, Smaragdov MG, Smirnov AF (1990) Intrachromosomal localization of the gene for the putative testis determining factor ion bovine prometaphasic chromosomes. Cytology (Russian) 32, 1182–1186
Mardon G, Page DC (1989) The sex-determining region of the mouse Y chromosome encodes a protein with a highly acidic domain and 13 zinc fingers. Cell 56, 765–770
Mardon G, Luoh S-W, Simpson EM, Gill G, Brown LG (1990). Mouse Zfx protein is similar Zfy-2: each contains an acidic activating domain and 13 zinc fingers. Mol Cell Biol 10, 681–688
Matsuda Y, Harada N-Y, Natsuume-sakai S, Lee K, Chapman VM (1992) Localization of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding. Cytogenet Cell Genet 61, 282–285
Page DC, Mosher R, Simpson EM, Fisher EMC, Mardon G, Pollack J, McGillivray B, de la Chapelle A, Brown LG (1987). The sexdetermining region of the human Y chromosome encodes a finger protein. Cell 51, 1091–1104
Palmer MS, Berta P, Sinclair AH, Pym B, Goodfellow PN (1990) Comparison of human ZFY and ZFX transcript. Proc Natl Acad Sci USA 87, 1681–1685
Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83, 2934–2938
Sambrook J, Fritsch EF, Maniatis T (1989) A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Schneider-Gadicke A, Beer-Romero P, Brown LG, Mardon G, Luoh S-W, Page DC (1989) Putative transcription activator with alternative isoforms encoded by human ZFX gene. Nature 342, 708–711
Shimmin LC, Chang BH-J, Li W-H (1993) Male-driven evolution of DNA sequences. Nature 362, 745–747
Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ, Foster JW, Frischauf A-M, Lovell-Badge R, Goodfellow PN (1990) A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif. Nature 346, 240–244
Takahashi E, Hori T, O’Cornell P, Leppert M, White R (1990) R banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1). Hum Genet 8, 14–16
Takahashi E, Yamauchi M, Tsuji H, Hitomi A, Meuth M, Hori T (1991) Chromosome mapping of the human cytidine-5′-triphosphate synthetase (CTPS) gene to band 1p34.1-p34.3 by fluorescence in situ hybridization. Hum Genet 88, 119–121
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to DDBJ, EMBL, and GenBank nucleotide sequence databases with the following accession numbers: D83489 for Zfy of the spinous country-rat and D84097 for bovine ZFX.
Rights and permissions
About this article
Cite this article
Xiao, C., Tsuchiya, K. & Sutou, S. Cloning and mapping of bovine ZFX gene to the long arm of the X-chromosome (Xq34) and homologous mapping of ZFY gene to the distal region of the short arm of the bovine (Yp13), ovine (Yp12–p13), and caprine (Yp12–p13) Y Chromosome. Mammalian Genome 9, 125–130 (1998). https://doi.org/10.1007/s003359900702
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900702