Skip to main content
SpringerLink
Log in

Chromosome mapping of the human cytidine-5′-triphosphate synthetase (CTPS) gene to band 1p34.1–p34.3 by fluorescence in situ hybridization

  • Short Communications
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

The human cytidine-5′-triphosphate synthetase (CTPS) gene was mapped by a direct mapping system combined with fluorescence in situ hybridization and replicated prometaphase R-bands. By high-resolution banding analysis, the signals were localized to band 34.1–34.3 of the short arm of chromosome 1; 1p34.1–p34.3. Simple procedures for the detection of R-bands are described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Ayusawa D, Takeishi K, Kanada S, Shimizu K, Koyama H, Seno T (1984) Isolation of functional cDNA clones for human thymidylate synthase. J Biol Chem 259:14361–14364

    Google Scholar 

  • Begley CG, Aplan PD, Davey MP, Nakahara K, Tchorz K, Kurtzberg J, Hershfield MS, Haynes BF, Cohen DI, Waldmann TA, Kirsch IR (1989) Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor δ-chain diversity region and results in a previously unreported fusion transcript. Proc Natl Acad Sci USA 86:2031–2035

    Google Scholar 

  • Bruns GAP, Sherman SL (1989) Report of the committee on the genetic constitution of chromosome 1. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:67–90

    Google Scholar 

  • Dracopoli NC, Harnett P, Bale SJ, Stanger BZ, Tucker MA, Housman DE, Kefford RF (1989) Loss of alleles from the distal short arm of chromosome 1 occurs late in melanoma tumor progression. Proc Natl Acad Sci USA 86:4616–4618

    Google Scholar 

  • Fong CT, Dracopoli NC, White PS, Merrill PT, Griffith RC, Housman DE, Brodeur GM (1989) Loss of heterozygosity for the short arm of chromosome 1 in human neuroblastomas: correlation with N-myc amplification. Proc Natl Acad Sci USA 86:3753–3757

    Google Scholar 

  • Goldman MA, Holmquist GP, Gray MC, Caston LA, Nag A (1984) Replication timing of genes and middle repetitve sequences. Science 224:686–692

    Google Scholar 

  • Holmquist G, Gray M, Porter T, Jordan J (1982) Characterization of Giemsa dark- and light-band DNA. Cell 31:121–129

    Google Scholar 

  • Hori T, Takahashi E, Ayusawa D, Takeishi K, Kaneda S, Seno T (1990) Regional assignment of the human thymidylate synthase (TS) gene to chromosome band 18p11.32 by nonisotopic in situ hybridization. Hum Genet 85:576–580

    Google Scholar 

  • Hurlbert RB, Kammen HO (1960) Formation of cytidine nucleotides from undine nucleotides by soluble mammalian enzymes: requirements for glutamine and guanosine nucleotides. J Biol Chem 235:443–449

    Google Scholar 

  • Landegent JE, Jansen in de Wal N, Dirks RW, Baas F, Ploeg M van der (1987) Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization. Hum Genet 77:366–370

    Google Scholar 

  • Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637

    Google Scholar 

  • Lawrence JB, Villnave CA, Singer RH (1988) Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell 52:51–61

    Google Scholar 

  • Leister I, Weith A, Brüderlein S, Cziepluch C, Kangwanpong D, Schlag P, Schwab M (1990) Human colorectal cancer: high frequency of deletions at chromosome 1p35. Cancer Res 50:7232–7235

    Google Scholar 

  • Lichter P, Chang CJ, Call K, Hermanson G, Evans GA, Housman D, Ward D (1990) High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69

    Google Scholar 

  • Lieberman I (1956) Enzymatic animation of uridine triphosphate to cytidine triphosphate. J Biol Chem 222:765–775

    Google Scholar 

  • Long CW, Pardee AB (1967) Cytidine triphosphate synthetase of Escherichia coli B. J Biol Chem 242:4715–4721

    Google Scholar 

  • Mathew CGP, Smith BA, Thorpe K, Wong Z, Royle NJ, Jeffreys AJ, Ponder BAJ (1987) Deletion of genes on chromosome 1 in endocrine neoplasia. Nature 328:524–526

    Google Scholar 

  • Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938

    Google Scholar 

  • Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA 85:9138–9142

    CAS  PubMed  Google Scholar 

  • Savage CR, Weinfeld H (1970) Purification and properties of mammalian liver cytidine triphosphate synthetase. J Biol Chem 245:2529–2535

    Google Scholar 

  • Sutherland GR, Ledbetter DH (1989) Report of the committee on cytogenetic markers. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:452–458

    Google Scholar 

  • Takahashi E, Hori T, Lawrence JB, McNeil J, Singer RH, O'Connell P, Leppert M, White R (1989) Human type II collagen gene (COL2A1) assigned to chromosome 12q13.1–q13.2 by in situ hybridization with biotinylated DNA probe. Jpn J Hum Genet 34:307–311

    Google Scholar 

  • Takahashi E, Hori T, O'Connell P, Leppert M, White R (1990a) R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1). Hum Genet 86:14–16

    Google Scholar 

  • Takahashi E, Hori T, Sutherland GR (1990b) Mapping of the human type II collagen gene (COL2A1) proximal to fra(12)(q13.1) by nonisotopic in situ hybridization. Cytogenet Cell Genet 54:84–85

    Google Scholar 

  • Takahashi E, Hori T, O'Connell P, Leppert M, White R (1991) Mapping of the MYC gene to band 8q24.12–q24.13 by R-banding and distal to fra(8)(q24.11), FRA8E, by fluorescence in situ hybridization. Cytogenet Cell Genet 57:109–111

    Google Scholar 

  • Tokino T, Takahashi E, Mori M, Tanigami A, Glaser T, Park JW, Jones C, Hori T, Nakamura Y (1991) Isolation and mapping of 62 new RFLP markers on human chromosome 11. Am J Hum Genet 48:258–268

    Google Scholar 

  • Trent JM, Kaneko Y, Mitelman F (1989) Report of the committee on structural chromosome changes in neoplasia. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:533–562

    Google Scholar 

  • Trudel M, Genechten TV, Meuth M (1984) Biochemical characterization of the hamster thy mutator gene and its revenants. J Biol Chem 259:2355–2359

    Google Scholar 

  • Weber G, Lui MS, Takeda E, Denton JE (1980) Enzymology of human colon tumours. Life Sci 27:793–799

    Google Scholar 

  • Weith A, Martinsson T, Cziepluch C, Bruderlein S, Amler LC, Berthold F, Schwab M (1989) Neuroblastoma concensus deletion maps to 1p36.1–2. Genes Chrom Canc 1:159–166

    Google Scholar 

  • Yamakawa K, Takahashi E, Saito H, Sato T, Oshimura M, Hori T, Nakamura Y (1991) Isolation and mapping of 75 new DNA markers on human chromosome 3. Genomics 9:536–543

    Google Scholar 

  • Yamauchi M, Yamauchi N, Meuth M (1990) Molecular cloning of the human CTP synthetase gene by functional complementation with purified human metaphase chromosomes. EMBO J 9:2095–2099

    Google Scholar 

  • Yamauchi M, Yamauchi N, Phear G, Spurr NK, Matinsson T, Weith A, Meuth M (1991) Genomic organization and chromosomal localization of the human CTP synthetase gene. Genomics (in press)

  • Yang KP, Nguyen CV, Castillo SG, Samaan NA (1990) Deletion mapping on the distal third of chromosome 1p in multiple endocrine neoplasia type IIA. Anticancer Res 10:527–534

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, 260, Chiba, Japan

    Ei-ichi Takahashi, Masatake Yamauchi, Hideo Tsuji, Akitsu Hitomi & Tada-aki Hori

  2. Imperial Cancer Research Fund, Clare Hall Laboratories, Blanche Lane, EN6 3LD, South Mimms, Herts, UK

    Mark Meuth

Authors
  1. Ei-ichi Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masatake Yamauchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hideo Tsuji
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akitsu Hitomi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mark Meuth
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tada-aki Hori
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takahashi, Ei., Yamauchi, M., Tsuji, H. et al. Chromosome mapping of the human cytidine-5′-triphosphate synthetase (CTPS) gene to band 1p34.1–p34.3 by fluorescence in situ hybridization. Hum Genet 88, 119–121 (1991). https://doi.org/10.1007/BF00204942

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00204942

Keywords

Search

Navigation