Abstract
Prostate cancer is not easy to metastasize because it is difficult to diagnose at an early stage, and there is no effective treatment currently. miRNA-217-5p has been reported to be a regulator in the process of prostate cancer. This study aimed to investigate how miRNA-217-5p affects the invasion and migration of prostate cancer. Luciferase assay was used to clarify whether the target gene Clusterin (CLU) was interacted directly with miR-217-5p. miR-217-5p and CLU were knocked down by transfecting respective siRNA into DU145 cells. The expression level of epithelial-mesenchymal transition (EMT)-related proteins was detected by Western blotting. Invasion and migration of DU145 cell were examined by wound healing assay. The results showed that miR-217-5p directly interacted with its target gene CLU, and the transfection of si-CLU and si-miR-217-5p had similar ability to regulate the expression level of EMT-related proteins, which in turn affected the migration and invasion of prostate cancer cell line DU145. In addition, miR-217-5p inhibited the expression of EMT-related proteins by regulating the expression of the target gene CLU, and further inhibited the invasion and migration of prostate cancer cells. Our findings provide a theoretical target basis for the treatment of prostate cancer.
Similar content being viewed by others
Change history
19 June 2023
This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s00335-023-10006-3
References
Ayyaz A, Kumar S, Sangiorgi B, Ghoshal B, Gosio J, Ouladan S, Fink M, Barutcu S, Trcka D, Shen J, Chan K, Wrana JL, Gregorieff A (2019) Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell. Nature 569:121–125
Bhandari V, Hoey C, Liu LY, Lalonde E, Ray J, Livingstone J, Lesurf R, Shiah YJ, Vujcic T, Huang X, Espiritu SMG, Heisler LE, Yousif F, Huang V, Yamaguchi TN, Yao CQ, Sabelnykova VY, Fraser M, Chua MLK, van der Kwast T, Liu SK, Boutros PC, Bristow RG (2019) Molecular landmarks of tumor hypoxia across cancer types. Nat Genet 51:308–318
Chayka O, Corvetta D, Dews M, Caccamo AE, Piotrowska I, Santilli G, Gibson S, Sebire NJ, Himoudi N, Hogarty MD, Anderson J, Bettuzzi S, Thomas-Tikhonenko A, Sala A (2009) Clusterin, a haploinsufficient tumor suppressor gene in neuroblastomas. J Natl Cancer Inst 101:663–677
Chen S, Huang V, Xu X, Livingstone J, Soares F, Jeon J, Zeng Y, Hua JT, Petricca J, Guo H, Wang M, Yousif F, Zhang Y, Donmez N, Ahmed M, Volik S, Lapuk A, Chua MLK, Heisler LE, Foucal A, Fox NS, Fraser M, Bhandari V, Shiah YJ, Guan J, Li J, Orain M, Picard V, Hovington H, Bergeron A, Lacombe L, Fradet Y, Tetu B, Liu S, Feng F, Wu X, Shao YW, Komor MA, Sahinalp C, Collins C, Hoogstrate Y, de Jong M, Fijneman RJA, Fei T, Jenster G, van der Kwast T, Bristow RG, Boutros PC, He HH (2019) Widespread and functional RNA circularization in localized prostate cancer. Cell 176:831-843 e822
Chi KN, Hotte SJ, Yu EY, Tu D, Eigl BJ, Tannock I, Saad F, North S, Powers J, Gleave ME, Eisenhauer EA (2010) Randomized phase II study of docetaxel and prednisone with or without OGX-011 in patients with metastatic castration-resistant prostate cancer. J Clin Oncol 28:4247–4254
Chi KN, Higano CS, Blumenstein B, Ferrero JM, Reeves J, Feyerabend S, Gravis G, Merseburger AS, Stenzl A, Bergman AM, Mukherjee SD, Zalewski P, Saad F, Jacobs C, Gleave M, de Bono JS (2017) Custirsen in combination with docetaxel and prednisone for patients with metastatic castration-resistant prostate cancer (SYNERGY trial): a phase 3, multicentre, open-label, randomised trial. Lancet Oncol 18:473–485
Gao P, Tchernyshyov I, Chang TC, Lee YS, Kita K, Ochi T, Zeller KI, De Marzo AM, Van Eyk JE, Mendell JT, Dang CV (2009) c-Myc suppression of miR-23a/b enhances mitochondrial glutaminase expression and glutamine metabolism. Nature 458:762–765
Gebert LFR, MacRae IJ (2019) Regulation of microRNA function in animals. Nat Rev Mol Cell Biol 20:21–37
Hussain M, Lin D, Saad F, Vapiwala N, Chapin BF, Sandler H, Evans CP, Carducci MA, Sachdev S (2020) Newly Diagnosed high-risk prostate cancer in an era of rapidly evolving new imaging: how do we treat? J Clin Oncol 39:1–13 (JCO2002268)
Li X, Li X, Ren Y, Yin Z, Quan X, Xue X, Zhou B (2017) Polymorphisms of rs1347093 and rs1397529 are associated with lung cancer risk in northeast Chinese population. Oncotarget 8:94862–94871
Liao S, Chen H, Liu M, Gan L, Li C, Zhang W, Lv L, Mei Z (2020) Aquaporin 9 inhibits growth and metastasis of hepatocellular carcinoma cells via Wnt/beta-catenin pathway. Aging 12:1527–1544
Liu X, Haniff HS, Childs-Disney JL, Shuster A, Aikawa H, Adibekian A, Disney MD (2020) Targeted degradation of the oncogenic microRNA 17–92 cluster by structure-targeting ligands. J Am Chem Soc 142:6970–6982
Mabe NW, Fox DB, Lupo R, Decker AE, Phelps SN, Thompson JW, Alvarez JV (2018) Epigenetic silencing of tumor suppressor Par-4 promotes chemoresistance in recurrent breast cancer. J Clin Invest 128:4413–4428
Park JH, Park JS, Ju SK, Lee KB, Park YK, Kang MH, Na SY, You KH (2003) Clusterin mRNA expression in apoptotic and activated rat thymocytes. Cell Res 13:49–58
Profumo V, Forte B, Percio S, Rotundo F, Doldi V, Ferrari E, Fenderico N, Dugo M, Romagnoli D, Benelli M, Valdagni R, Dolfini D, Zaffaroni N, Gandellini P (2019) LEADeR role of miR-205 host gene as long noncoding RNA in prostate basal cell differentiation. Nat Commun 10:307
Rokavec M, Oner MG, Li H, Jackstadt R, Jiang L, Lodygin D, Kaller M, Horst D, Ziegler PK, Schwitalla S, Slotta-Huspenina J, Bader FG, Greten FR, Hermeking H (2014) IL-6R/STAT3/miR-34a feedback loop promotes EMT-mediated colorectal cancer invasion and metastasis. J Clin Invest 124:1853–1867
Seo JA, Kang MC, Yang WM, Hwang WM, Kim SS, Hong SH, Heo JI, Vijyakumar A, Pereira de Moura L, Uner A, Huang H, Lee SH, Lima IS, Park KS, Kim MS, Dagon Y, Willnow TE, Aroda V, Ciaraldi TP, Henry RR, Kim YB (2020) Apolipoprotein J is a hepatokine regulating muscle glucose metabolism and insulin sensitivity. Nat Commun 11:2024
Stuelten CH, Parent CA, Montell DJ (2018) Cell motility in cancer invasion and metastasis: insights from simple model organisms. Nat Rev Cancer 18:296–312
Sutaria DS, Jiang J, Azevedo-Pouly AC, Wright L, Bray JA, Fredenburg K, Liu X, Lu J, Torres C, Mancinelli G, Grippo PJ, Coppola V, Schmittgen TD (2019) Knockout of acinar enriched microRNAs in mice promote duct formation but not pancreatic cancer. Sci Rep 9:11147
Vo JN, Cieslik M, Zhang Y, Shukla S, Xiao L, Zhang Y, Wu YM, Dhanasekaran SM, Engelke CG, Cao X, Robinson DR, Nesvizhskii AI, Chinnaiyan AM (2019) The landscape of circular RNA in cancer. Cell. 176:869-881 e813
Wang Z, Xia F, Labib M, Ahmadi M, Chen H, Das J, Ahmed SU, Angers S, Sargent EH, Kelley SO (2020) Nanostructured architectures promote the mesenchymal-epithelial transition for invasive cells. ACS Nano 14:5324–5336
Warri AM, Huovinen RL, Laine AM, Martikainen PM, Harkonen PL (1993) Apoptosis in toremifene-induced growth inhibition of human breast cancer cells in vivo and in vitro. J Natl Cancer Inst 85:1412–1418
Wencong M, Jinghan W, Yong Y, Jianyang A, Bin L, Qingbao C, Chen L, Xiaoqing J (2020) FOXK1 promotes proliferation and metastasis of gallbladder cancer by activating AKT/mTOR signaling pathway. Front Oncol 10:545
Wittmann D, Skolarus T, Glode LM (2020) Reconsidering the trade-offs of prostate cancer screening. N Engl J Med 383:1289–1290
Woodcock DJ, Riabchenko E, Taavitsainen S, Kankainen M, Gundem G, Brewer DS, Ellonen P, Lepisto M, Golubeva YA, Warner AC, Tolonen T, Jasu J, Isaacs WB, Emmert-Buck MR, Nykter M, Visakorpi T, Bova GS, Wedge DC (2020) Prostate cancer evolution from multilineage primary to single lineage metastases with implications for liquid biopsy. Nat Commun 11:5070
Xhemalce B, Robson SC, Kouzarides T (2012) Human RNA methyltransferase BCDIN3D regulates microRNA processing. Cell 151:278–288
Xu X, Song F, Jiang X, Hong H, Fei Q, Jin Z, Zhu X, Dai B, Yang J, Sui C, Xu M (2020) Long non-coding RNA SNHG14 contributes to the development of hepatocellular carcinoma via sponging miR-217. Onco Targets Ther 13:4865–4876
Yang X, Li D, Qi YZ, Chen W, Yang CH, Jiang YH (2020) MicroRNA-217 ameliorates inflammatory damage of endothelial cells induced by oxidized LDL by targeting EGR1. Mol Cell Biochem. https://doi.org/10.1007/s11010-020-03857-w
Zhang F, Kumano M, Beraldi E, Fazli L, Du C, Moore S, Sorensen P, Zoubeidi A, Gleave ME (2014) Clusterin facilitates stress-induced lipidation of LC3 and autophagosome biogenesis to enhance cancer cell survival. Nat Commun 5:5775
Zhou P, Xie W, Huang HL, Huang RQ, Tian C, Zhu HB, Dai YH, Li ZY (2020) circRNA_100859 functions as an oncogene in colon cancer by sponging the miR-217-HIF-1alpha pathway. Aging 12:13338–13353
Zhou S, Zhu C, Pang Q, Liu HC (2021) MicroRNA-217: a regulator of human cancer. Biomed Pharmacother 133:110943
Zhu J, Zhou L, Wei B, Qian Z, Wang J, Hui H, Sun Y (2020) miR1425p inhibits pancreatic cancer cell migration and invasion by targeting PIK3CA. Mol Med Rep 22:2085–2092
Funding
None.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Informed Consent
Informed consent was obtained from all individual participants included in the study.
Research Involving Human Participants
The patient’s written informed consent was obtained, and the study was approved by the Institutional Human Experiment and Ethics Committee of the Third Hospital of Hebei Medical University. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1007/s00335-023-10006-3"
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Zhao, W., Wang, X., Jiang, Y. et al. RETRACTED ARTICLE: miR-217-5p Inhibits Invasion and Metastasis of Prostate Cancer by Targeting Clusterin. Mamm Genome 32, 371–380 (2021). https://doi.org/10.1007/s00335-021-09874-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00335-021-09874-4