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MiR-378 suppresses prostate cancer cell growth through downregulation of MAPK1 in vitro and in vivo

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Tumor Biology

Abstract

Prostate cancer is one of the biggest health problems for the aging male. To the present, the roles of dysregulated microRNAs in prostate cancer are still unclear. Here, we evaluated the anti-proliferative role of miR-378 in prostate cancer. And, we found that the expression of miR-378 was significantly downregulated in clinical prostate cancer tissues. In vitro assay suggested that overexpression of miR-378-suppressed prostate cancer cell migration and invasion promoted cell apoptosis. Furthermore, we identified and validated MAPK1 as a direct target of miR-378. Ectopic expression of MAPK1 rescues miR-378-suppressed cell migration and invasion. In vivo assay demonstrated that the stably miR-378-overexpressed prostate cancer cells displayed a significantly reduction in tumor growth. Taken together, our data suggested that miR-378 may act as a potential therapeutic target against human prostate cancer.

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Authors and Affiliations

  1. Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China

    Qi-guang Chen, Shu-qi Du, Zhen-hua Li, Zhe Zhang & Chui-ze Kong

  2. Department of Diagnostic Radiology, General Hospital of Shenyang Military Region, Shenyang, Liaoning, China

    Wei Zhou

  3. Department of Oncology, General Hospital of Shenyang Military Region, Shenyang, Liaoning, China

    Tao Han

  4. Department of Urology, LiaoNing Cancer Hospital and Institute, Shenyang, Liaoning, China

    Guang-yi Shan

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  1. Qi-guang Chen
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  2. Wei Zhou
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  3. Tao Han
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  4. Shu-qi Du
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  5. Zhen-hua Li
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  6. Zhe Zhang
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  7. Guang-yi Shan
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  8. Chui-ze Kong
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Correspondence to Chui-ze Kong.

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Chen, Qg., Zhou, W., Han, T. et al. MiR-378 suppresses prostate cancer cell growth through downregulation of MAPK1 in vitro and in vivo. Tumor Biol. 37, 2095–2103 (2016). https://doi.org/10.1007/s13277-015-3996-8

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  • DOI: https://doi.org/10.1007/s13277-015-3996-8

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