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Pseudogenes of rat VDAC1: 16 gene segments in the rat genome show structural similarities with the cDNA encoding rat VDAC1, with 8 slightly expressed in certain tissues

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Abstract

BLAST analysis of the rat genome revealed the presence of 16 pseudogenes of isoform 1 of the mitochondrial voltage-dependent anion channel (VDAC1). Based on their structural characterization, it was concluded that these pseudogenes were formed by integration of VDAC1 cDNA into the genome, and subsequent rearrangements/mutations. By RT-PCR analysis using carefully designed primers that could not amplify the cDNA of genuine VDAC1, 8 of these 16 pseudogenes showed slight expression in certain tissues, but none of them seemed to encode a functional protein.

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Authors and Affiliations

  1. Institute for Genome Research, University of Tokushima, Kuramoto-cho-3, Tokushima, 770-8503, Japan

    Yusuke Ido, Takenori Yamamoto, Tatsuki Yoshitomi, Atsushi Yamamoto, Eriko Obana & Yasuo Shinohara

  2. Graduate School of Pharmaceutical Science, University of Tokushima, Shomachi-1, Tokushima, 770-8505, Japan

    Yusuke Ido, Tatsuki Yoshitomi, Atsushi Yamamoto, Eriko Obana & Yasuo Shinohara

  3. Faculty of Pharmaceutical Science, Suzuka University of Medical Science, Minamitamagaki-cho-3500, Suzuka, 513-8670, Japan

    Kazuto Ohkura

Authors
  1. Yusuke Ido
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  2. Takenori Yamamoto
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  3. Tatsuki Yoshitomi
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  4. Atsushi Yamamoto
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  5. Eriko Obana
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  6. Kazuto Ohkura
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  7. Yasuo Shinohara
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Corresponding author

Correspondence to Yasuo Shinohara.

Additional information

Y. Ido and T. Yamamoto contributed equally to this work.

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Ido, Y., Yamamoto, T., Yoshitomi, T. et al. Pseudogenes of rat VDAC1: 16 gene segments in the rat genome show structural similarities with the cDNA encoding rat VDAC1, with 8 slightly expressed in certain tissues. Mamm Genome 23, 286–293 (2012). https://doi.org/10.1007/s00335-011-9375-x

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  • DOI: https://doi.org/10.1007/s00335-011-9375-x

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