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Classification of FABP isoforms and tissues based on quantitative evaluation of transcript levels of these isoforms in various rat tissues

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Abstract

In mammals, 10 isoforms of fatty acid-binding protein (FABP) are expressed in various tissues. To understand the role of multiple FABP isoforms, we have quantitatively examined the transcript levels of individual FABP isoforms in each of various tissues by Northern blotting using synthesized RNAs corresponding to the mRNA of each isoform as external standards. As a result, absolute transcript levels of individual FABP isoforms expressed in each tissue were successfully determined. The 10 FABP isoforms were classified into three categories: (1) isoforms FABP7 and 12 were not markedly expressed in any tissue examined; (2) isoforms showing certain transcript levels in multiple tissues; and (3) isoforms FABP6, 8, and 9, expressed at certain levels in one particular tissue. Based on the expression profiles of the isoforms, individual tissues were also classified into three groups: (1) tissues in which high-level expression of FABP isoforms was not observed, (2) tissues in which multiple FABP isoforms were expressed at certain levels, and (3) tissues in which a single FABP isoform was dominantly expressed at a certain level. These results give a better understanding of the meaning of the presence of multiple FABP isoforms in mammals.

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Authors and Affiliations

  1. Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima, 770-8503, Japan

    Takenori Yamamoto, Atsushi Yamamoto, Masahiro Watanabe, Taisuke Matsuo & Yasuo Shinohara

  2. Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi-1, Tokushima, 770-8505, Japan

    Atsushi Yamamoto, Masahiro Watanabe, Taisuke Matsuo, Naoshi Yamazaki & Yasuo Shinohara

  3. Health Technology Research Center, National Institute for Advanced Industrial Science and Technology (AIST), 2217-14 Hayashicho, Takamatsu, 761-0395, Japan

    Masahiro Watanabe, Masatoshi Kataoka & Yasuo Shinohara

  4. Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda, 278-8510, Japan

    Hiroshi Terada

Authors
  1. Takenori Yamamoto
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  2. Atsushi Yamamoto
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  3. Masahiro Watanabe
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  4. Taisuke Matsuo
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  5. Naoshi Yamazaki
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  6. Masatoshi Kataoka
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  7. Hiroshi Terada
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  8. Yasuo Shinohara
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Correspondence to Yasuo Shinohara.

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Yamamoto, T., Yamamoto, A., Watanabe, M. et al. Classification of FABP isoforms and tissues based on quantitative evaluation of transcript levels of these isoforms in various rat tissues. Biotechnol Lett 31, 1695–1701 (2009). https://doi.org/10.1007/s10529-009-0065-7

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  • DOI: https://doi.org/10.1007/s10529-009-0065-7

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