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One-step transformation of two Andean potato cultivars (Solanum tuberosum L. subsp. andigena)

  • Genetic Transformation and Hybridization
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Abstract.

Most Agrobacterium-mediated transformation procedures described in the literature have proven to be inefficient for transformation of Andean potato cultivars. We describe here a more successful method for transformation of two of these cultivars, Diacol Capiro (DC) and Parda Pastusa (PP). Leaf explants were transformed using vector pBI121 carrying the β-glucuronidase and kanamycin resistance genes. Calli and shoot formation occurred after 5–8 weeks on a selection medium containing 3 mg/l zeatin and 1 mg/l indole acetic acid. The shoot regeneration frequency was 28% and 34% for DC and PP, respectively. Of the kanamycin-resistant plantlets obtained, 51% of those of DC and 13% of those of PP were confirmed to be transformants by histochemical assays, polymerase chain reaction analyses and Southern blotting techniques.

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Author notes

  1. R. Arango

    Present address: Departimento de Biología, Facultad de Ciencias, Universidad Nacional sede Medellín, Calle 64x Cra 65 Autopista, Norte Medellín, Colombia

Authors and Affiliations

  1. Unidad de Biotecnología Vegetal, Corporación para Investigaciones Biológicas, (CIB)/Departamento de Biología, Universidad de Antioquia, Antioquia, Colombia

    C. Trujillo

  2. Corporación para Investigaciones Biológicas, (CIB), Unidad de Biotecnología Vegetal, Carrera 72A No 78B-141, Medellin, Colombia

    E. Rodríguez-Arango & R. Arango

  3. Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia sede Medellín, Calle 64x, Cra 65 Autopista, Norte Medellin, Colombia

    S. Jaramillo & R. Hoyos

  4. Corporación para Investigaciones Biológicas, (CIB), Unidad de Control Biológico, Carrera 72A No 78B-141, Medellin, Colombia

    S. Orduz

Authors
  1. C. Trujillo
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  2. E. Rodríguez-Arango
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  3. S. Jaramillo
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  4. R. Hoyos
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  5. S. Orduz
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  6. R. Arango
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Revision received: 6 July 2001

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Trujillo, C., Rodríguez-Arango, E., Jaramillo, S. et al. One-step transformation of two Andean potato cultivars (Solanum tuberosum L. subsp. andigena). Plant Cell Rep 20, 637–641 (2001). https://doi.org/10.1007/s002990100381

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  • DOI: https://doi.org/10.1007/s002990100381

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