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In vitro pollination of isolated ovules of Cichorium intybus L.

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Abstract

 A method for the in vitro pollination of chicory (Cichorium intybus L.) ovules was developed. The purpose was to avoid self-incompatibility after in vitro self-pollination of ovules isolated from flower buds before anthesis and from open flower heads. Seedlings were obtained at a low percentage (0.76%), and the results were explained in terms of pollen viability, pollen germination on the ovule, embryogenesis studies and ploidy analysis.

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Authors and Affiliations

  1. Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Willem de Croylaan 42, B-3001 Heverlee, Belgium e-mail: claracastano@yahoo.com Fax: +32-16-322966, , , , , , BE

    C. I. Castaño & M. P. DeProft

Authors
  1. C. I. Castaño
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  2. M. P. DeProft
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Received: 9 April 1997 / Revision received: 23 July 1997 / Accepted: 6 September 1999

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Castaño, C., DeProft, M. In vitro pollination of isolated ovules of Cichorium intybus L.. Plant Cell Reports 19, 616–621 (2000). https://doi.org/10.1007/s002990050782

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  • DOI: https://doi.org/10.1007/s002990050782

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