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Resistance to Botrytis cinerea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-AMP1

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Abstract

Scented geranium (Pelargonium sp. `Fren-sham') was transformed with a gene encoding an antimicrobial protein (Ace-AMP1) from onion through an Agrobacterium-mediated transformation system. The binary vector pFAJ3033 contained the coding region of the Ace-AMP1 preproprotein-encoding cDNA. Transformants were verified by polymerase chain reaction and Southern blot analysis. Transgenic plants expressing high levels of Ace-AMP1 were identified by immunoblots and those plants were shown to have increased resistance to Botrytis cinerea leaf infection.

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Authors and Affiliations

  1. Department of Horticultural Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Fax: 519-767-0755 e-mail: psaxena@uoguelph.ca, , , , , , CA

    Y.-M. Bi, S. KrishnaRaj & P. K. Saxena

  2. F. A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, K. Mercierlaan 92, B-3001 Heverlee, Belgium, , , , , , BE

    B. P. A. Cammue

  3. Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1, , , , , , CA

    P. H. Goodwin

Authors
  1. Y.-M. Bi
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  2. B. P. A. Cammue
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  3. P. H. Goodwin
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  4. S. KrishnaRaj
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  5. P. K. Saxena
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Additional information

Received: 23 July 1998 / Revision received: 24 November 1998 / Accepted: 5 December 1998

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Bi, YM., Cammue, B., Goodwin, P. et al. Resistance to Botrytis cinerea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-AMP1. Plant Cell Reports 18, 835–840 (1999). https://doi.org/10.1007/s002990050670

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  • DOI: https://doi.org/10.1007/s002990050670

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