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A simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula××intermedia Emeric ex Loiseleur)

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Abstract

A shoot regeneration system from leaves of lavandin (Lavandula×intermedia Emeric ex Loiseleur) was developed. The best results were obtained using a set of four different media. Callus was obtained on Murashige and Skoog (MS) medium containing 9 μm 6-benzylaminopurine and 4.5 μm α-naphthaleneacetic acid. After 2 weeks of culture, calli were transferred onto MS medium supplemented with 18 μm 6-benzylaminopurine to trigger bud regeneration. Shoot elongation was then stimulated by 1 μm gibberellin A3. Rooting was induced with 1 μm indole-3-butyric acid. All plantlets survived to greenhouse acclimatization. This is the first description of bud regeneration from leaves of lavandin.

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Authors and Affiliations

  1. Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et Médicinales, Faculté des Sciences et Techniques, Université Jean Monnet, 23 rue du Dr Michelon, F-42023 Saint-Etienne Cédex 2, France e-mail: dronne@univ-st-etienne.fr Fax: +33-4-77251817, , , , , , FR

    S. Dronne, F. Jullien, J.-C. Caissard & O. Faure

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  1. S. Dronne
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  2. F. Jullien
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  3. J.-C. Caissard
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  4. O. Faure
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Received: 23 May 1997 / Revision received: 11 September 1997 / Accepted: 18 October 1997

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Dronne, S., Jullien, F., Caissard, JC. et al. A simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula××intermedia Emeric ex Loiseleur). Plant Cell Reports 18, 429–433 (1999). https://doi.org/10.1007/s002990050598

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  • DOI: https://doi.org/10.1007/s002990050598

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