Abstract
A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme β-glucuronidase (GUS) and introduced into tobacco via Agrobacterium-mediated transformation. The promoter fragment directed expression of the GUS gene in the stigma of transgenic tobacco. Some transformants also showed relatively low GUS activity in the pollen.
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Received: 25 May 1998 / Revision received: 30 July 1998 / Accepted: 21 August 1998
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Robert, L., Lévesque-Lemay, M., Gerster, J. et al. Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma. Plant Cell Reports 18, 357–362 (1999). https://doi.org/10.1007/s002990050586
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DOI: https://doi.org/10.1007/s002990050586