Abstract.
A highly efficient in vitro regeneration methodology for mature chinese tallow tree (Sapium sebiferum Roxb.) has been developed. Shoot segments cultured on MS medium supplemented with 7.5 μM NAA produced light green callus. Optimum shoot differentiation resulted when callus was transferred to MS medium with 1 μM BA and 0.25 μM NAA. Shoot forming ability of callus was higher on MS medium compared to B5, half-MS or WPM. A continuous shoot harvest system at four-week intervals was established. Shoot yield continued for six months without loss of vigour. Regenerated shoots were rooted by culturing on half-strength agar-gelled MS medium containing 1 μM IBA. Rooted plantlets were transferred to 1:1 soil vermiculite mixture and acclimatized with 67% survival rate. Fully acclimatized plants were planted in the field, and performance is being evaluated.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received 4 January 1996/Revised version received 18 April 1996 – Communicated by W. Parrott
Rights and permissions
About this article
Cite this article
Siril, E., Dhar, U. A highly efficient in vitro regeneration methodology for mature Chinese tallow tree (Sapium sebiferum Roxb.). Plant Cell Reports 16, 83–87 (1996). https://doi.org/10.1007/s002990050182
Issue Date:
DOI: https://doi.org/10.1007/s002990050182