Abstract
Key message
Sucrose invertase activity is positively related to osmotic and salt stress resistance in peanut.
Abstract
Sucrose invertases (INVs) have important functions in plant growth and response to environmental stresses. However, their biological roles in peanut are still not fully revealed. In this research, we identified 42 AhINV genes in the peanut genome. They were highly conserved and clustered into three groups with 24 segmental duplication events occurred under purifying selection. Transcriptional expression analysis exhibited that they were all ubiquitously expressed, and most of them were up-regulated by osmotic and salt stresses, with AhINV09, AhINV23 and AhINV19 showed the most significant up-regulation. Further physiochemical analysis showed that the resistance of peanut to osmotic and salt stress was positively related to the high sugar content and sucrose invertase activity. Our results provided fundamental information on the structure and evolutionary relationship of INV gene family in peanut and gave theoretical guideline for further functional study of AhINV genes in response to abiotic stress.
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Funding
This work has been jointly supported by the following grants: The Key Research and Development Project of Shandong Province (2020LZGC001); The Natural Science Foundation of Shandong Province, China (ZR2021QC140); The National Mega Project of GMO Crops of China (2016ZX08004-002-006); The National Natural Science Foundation of China (32071733); The Cooperation Project of University and Local Enterprise in Yantai of Shandong Province (2021XDRHXMPT09).
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Juan Zhang and HongXia Zhang designed the experiments; Yaru Zhang, Wenwen Xue, Yu Jin, Hongfei Zhao and Yibo Wang performed the experiments; Tingting Mao, Yaru Zhang, Shengnan Wang, Shengjie Zhuo, Feifei Gao and Yanping Su analysed the data; Chunyan Yu and Xiaotong Guo supervised the experiment; Yuting Sheng supported the experiment. Tingting Mao wrote the manuscript; Juan Zhang and Hongxia Zhang revised the manuscript. All authors read and approved the paper.
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Mao, T., Zhang, Y., Xue, W. et al. Identification, characterisation and expression analysis of peanut sugar invertase genes reveal their vital roles in response to abiotic stress. Plant Cell Rep 43, 30 (2024). https://doi.org/10.1007/s00299-023-03123-5
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DOI: https://doi.org/10.1007/s00299-023-03123-5