Abstract
Key message
Multiple regulatory pathways of T. chinensis to salt stress were identified through transcriptome data analysis.
Abstract
Tamarix chinensis (Tamarix chinensis Lour.) is a typical halophyte capable of completing its life cycle in soils with medium to high salinity. However, the mechanisms underlying its resistance to high salt stress are still largely unclear. In this study, transcriptome profiling analyses in different organs of T. chinensis plants in response to salt stress were carried out. A total number of 2280, 689, and 489 differentially expressed genes (DEGs) were, respectively, identified in roots, stems, and leaves, with more DEGs detected in roots than in stems and leaves. Gene Ontology (GO) term analysis revealed that they were significantly enriched in “biological processes” and “molecular functions”. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that “Beta-alanine metabolism” was the most differentially enriched pathway in roots, stems, and leaves. In pair-to-pair comparison of the most differentially enriched pathways, a total of 14 pathways, including 5 pathways in roots and leaves, 6 pathways in roots and stems, and 3 pathways in leaves and stems, were identified. Furthermore, genes encoding transcription factor, such as bHLH, bZIP, HD-Zip, MYB, NAC, WRKY, and genes associated with oxidative stress, starch and sucrose metabolism, and ion homeostasis, were differentially expressed with distinct organ specificity in roots, stems, and leaves. Our findings in this research provide a novel approach for exploring the salt tolerance mechanism of halophytes and identifying new gene targets for the genetic breeding of new plant cultivars with improved resistance to salt stress.
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Data availability statement
The datasets presented in this study can be found at the website https://www.ncbi.nlm.nih.gov/bioproject/ with the accession number of PRJNA991128.
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This work has been jointly supported by the following grants: the Natural Science Foundation of Shandong Province, China (ZR2020QC062); the Innovation Project of Shandong Academy of Agricultural Sciences, China (CXGC2022F06, CXGC2023A16, CXGC2023G07); the National Natural Science Foundation of China (41807083, 32071733, 31870576); the “Cocoon-break” Supporting Fund Project of Shandong Institute of Sericulture, China (FC202301).
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HZ and HZ conceived and designed the experiments; RL, RF, ML, YS, JL, CC, YG, XL, WN, LM, and XW performed the experiments and data analysis. HZ and HZ wrote and revised the manuscript. All authors have read and approved the paper.
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Li, R., Fu, R., Li, M. et al. Transcriptome profiling reveals multiple regulatory pathways of Tamarix chinensis in response to salt stress. Plant Cell Rep 42, 1809–1824 (2023). https://doi.org/10.1007/s00299-023-03067-w
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DOI: https://doi.org/10.1007/s00299-023-03067-w