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Identification and promoter analysis of a GA-stimulated transcript 1 gene from Jatropha curcas

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Abstract

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Overexpression of JcGAST1 promotes plant growth but inhibits pistil development. The pyrimidine box and CGTCA motif of the JcGAST1 promoter were responsible for the GA and MeJA responses.

Abstract

Members of the gibberellic acid-stimulated Arabidopsis (GASA) gene family play roles in plant growth and development, particularly in flower induction and seed development. However, there is still relatively limited knowledge of GASA genes in Jatropha curcas. Herein, we identified a GASA family gene from Jatropha curcas, namely, JcGAST1, which encodes a protein containing a conserved GASA domain. Sequence alignment showed that the JcGAST1 protein shares 76% sequence identity and 80% sequence similarity with SlGAST1. JcGAST1 had higher expression and protein levels in the female flowers than in the male flowers. Overexpression of JcGAST1 in tobacco promotes plant growth but inhibits pistil development. JcGAST1 expression was upregulated by GA and downregulated by MeJA. Promoter analysis indicated that the pyrimidine box and CGTCA motif were the GA- and MeJA-responsive elements of the JcGAST1 promoter. Using a Y1H screen, six transcription factors were found to interact with the pyrimidine box, and three transcription factors were found to interact with the CGTCA motif. Overall, the results of this study improve our understanding of the JcGAST1 gene and provide useful information for further studies.

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All datasets generated for this study are included in the article/Supplementary Files.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant No, 31760198).

Funding

This study was funded by the National Natural Science Foundation of China (grant No, 31760198).

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Author notes

  1. Shikang Lei and Liangqing Zhao contributed equally to this study.

Authors and Affiliations

  1. School of Traditional Chinese Medicine Resource/ Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Shikang Lei & Gang Xu

  2. Key Laboratory of Biology and Genetic Breeding, Guangzhou Academy of Agricultural Sciences, Guangzhou, 510000, China

    Shikang Lei

  3. Guizhou Botanical Garden, Guiyang, 550005, China

    Liangqing Zhao

  4. Institute for Forest Resources and Environment of Guizhou/College of Forestry, Guizhou University, Guiyang, 550025, China

    Yuqian Chen

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  1. Shikang Lei
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  2. Liangqing Zhao
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  4. Gang Xu
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Contributions

GX: designed and managed this study; SL, LZ, and YC: performed the experiments; LZ and YC: analyzed the data; SL and GX: wrote the manuscript; all authors read and approved the manuscript.

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Correspondence to Gang Xu.

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Communicated by Jinghua Yang.

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Lei, S., Zhao, L., Chen, Y. et al. Identification and promoter analysis of a GA-stimulated transcript 1 gene from Jatropha curcas. Plant Cell Rep 42, 1333–1344 (2023). https://doi.org/10.1007/s00299-023-03034-5

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