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The SMC5/6 complex subunit MMS21 regulates stem cell proliferation in rice

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Abstract

Key message

SMC5/6 complex subunit OsMMS21 is involved in cell cycle and hormone signaling and required for stem cell proliferation during shoot and root development in rice.

Abstract

The structural maintenance of chromosome (SMC)5/6 complex is required for nucleolar integrity and DNA metabolism. Moreover, METHYL METHANESULFONATE SENSITIVITY GENE 21 (MMS21), a SUMO E3 ligase that is part of the SMC5/6 complex, is essential for the root stem cell niche and cell cycle transition in Arabidopsis. However, its specific role in rice remains unclear. Here, OsSMC5 and OsSMC6 single heterozygous mutants were generated using CRISPR/Cas9 technology to elucidate the function of SMC5/6 subunits, including OsSMC5, OsSMC6, and OsMMS21, in cell proliferation in rice. ossmc5/ + and ossmc6/ + heterozygous single mutants did not yield homozygous mutants in their progeny, indicating that OsSMC5 and OsSMC6 both play necessary roles during embryo formation. Loss of OsMMS21 caused severe defects in both the shoot and roots in rice. Transcriptome analysis showed a significant decrease in the expression of genes involved in auxin signaling in the roots of osmms21 mutants. Moreover, the expression levels of the cycB2-1 and MCM genes, which are involved the cell cycle, were significantly lower in the shoots of the mutants, indicating that OsMMS21 was involved in both hormone signaling pathways and the cell cycle. Overall, these findings indicate that the SUMO E3 ligase OsMMS21 is required for both shoot and root stem cell niches, improving the understanding of the function of the SMC5/6 complex in rice.

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The authors confirm that all relevant data supporting the findings of this study are available within the article and its supplementary material. Raw data that supports the findings of this study are available from the corresponding author, upon reasonable request.

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Acknowledgements

The authors thank Pro. Jiankang Zhu and Pro. Caixia Gao for offering the CRISPR/Cas9 gene-editing vector. We also appreciate the Biogle and Biorun Geneme Editing Center for producing transgenic rice.

Funding

This study was supported by the National Natural Science Foundation of China (31971842).

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Authors and Affiliations

  1. College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China

    Qian Xun, Ye Song, Min Mei, Yanfeng Ding & Chengqiang Ding

  2. Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture, Nanjing, 210095, China

    Yanfeng Ding & Chengqiang Ding

  3. Jiangsu Collaborative Innovation Centre for Modern Crop Production, Nanjing, 210095, China

    Yanfeng Ding & Chengqiang Ding

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  1. Qian Xun
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  2. Ye Song
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  3. Min Mei
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  4. Yanfeng Ding
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  5. Chengqiang Ding
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Contributions

CD and YD designed the research. QX, YS, MM, and CD performed the experiments. QX and CD analyzed the data and prepared the paper.

Corresponding author

Correspondence to Chengqiang Ding.

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The authors declare no conflict of interest.

Accession numbers

Gene information in this article can be found in Rice Annotation Project database, miRbase data libraries and the Arabidopsis Information Resource are under the corresponding accession numbers: OsActin (Os03g0718100), OsMMS21(Os05g0563500), OsSMC5 (Os05g0596600), OsSMC6 (Os09g0121050), cycb2-1 (Os04g0563700), OsERF3 (Os01g0797600), OsPIN9 (Os01g0802700), OsMADS25 (Os04g0304400), SMOS1 (Os05g0389000), AIM1 (Os02g0274100).

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Communicated by Wei Ma.

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Xun, Q., Song, Y., Mei, M. et al. The SMC5/6 complex subunit MMS21 regulates stem cell proliferation in rice. Plant Cell Rep 42, 1279–1290 (2023). https://doi.org/10.1007/s00299-023-03030-9

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