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Plant deubiquitinases: from structure and activity to biological functions

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Abstract

Key message

This article attempts to provide comprehensive review of plant deubiquitinases, paying special attention to recent advances in their biochemical activities and biological functions.

Abstract

Proteins in eukaryotes are subjected to post-translational modifications, in which ubiquitination is regarded as a reversible process. Cellular deubiquitinases (DUBs) are a key component of the ubiquitin (Ub)–proteasome system responsible for cellular protein homeostasis. DUBs recycle Ub by hydrolyzing poly-Ub chains on target proteins, and maintain a balance of the cellular Ub pool. In addition, some DUBs prefer to cleave poly-Ub chains not linked through the conventional K48 residue, which often alter the substrate activity instead of its stability. In plants, all seven known DUB subfamilies have been identified, namely Ub-binding protease/Ub-specific protease (UBP/USP), Ub C-terminal hydrolase (UCH), Machado–Joseph domain-containing protease (MJD), ovarian-tumor domain-containing protease (OTU), zinc finger with UFM1-specific peptidase domain protease (ZUFSP), motif interacting with Ub-containing novel DUB family (MINDY), and JAB1/MPN/MOV34 protease (JAMM). This review focuses on recent advances in the structure, activity, and biological functions of plant DUBs, particularly in the model plant Arabidopsis.

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Data availability

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors wish to thank Yingya Gong and Yuepeng Zang for sharing unpublished observations and other Xiao laboratory members for discussion.

Funding

This work was supported by the Capital Normal University and a Saskatchewan Canola Development Commission grant ADF 20210893 to WX.

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  1. Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, 100048, China

    Runbang Luo, Kun Yang & Wei Xiao

  2. Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada

    Runbang Luo, Kun Yang & Wei Xiao

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  1. Runbang Luo
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RL and WX conceived and designed research. RL and KY collected and analyzed data. LW, KY and WX wrote the manuscript. All authors read and approved the manuscript.

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Luo, R., Yang, K. & Xiao, W. Plant deubiquitinases: from structure and activity to biological functions. Plant Cell Rep 42, 469–486 (2023). https://doi.org/10.1007/s00299-022-02962-y

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