Abstract
Key Message
We investigated the mechanism of the effect of different light qualities on the synthesis and regulation of mung bean sprouts. Light quality acts as a signal molecule, strongly enhancing polyphenol biosynthesis in sprouts.
Abstract
Mung bean (Vigna radiata) sprouts are a popular sprouting vegetable all over the world and are an excellent source of polyphenols with high antioxidant activity. This study investigated the effects of light qualities on the kinetic changes and metabolic regulation mechanism of light signal-mediating polyphenols in three mung bean sprout cultivars. Experimental results showed that three light qualities significantly enhanced the contents of caffeic acid, rutin, vitexin, genistin and delphinidin 3-glucoside. Interestingly, ferulic acid and vitexin responded selectively to blue light and red light, severally. Most genes involved in polyphenol biosynthesis were activated under different light quality conditions, resulting in an overaccumulation of phenylpropanoids. Pearson correlation analysis showed that PAL, F3H, F3’H and F3′5’H expression correlated highly with rutin, whereas ANS expression paralleled anthocyanin biosynthesis. Moreover, MYB111, MYB3, MYB4, MYB1 and MYC2 were critical regulators of polyphenol biosynthesis in mung bean sprouts. These changes were likely due to the changes in the expression of the photoreceptor genes CRY-D, PHOT2, PHYE and light response genes (PIF3 and HY5). Our results provide insights into polyphenol biosynthesis in sprouts and microgreens.
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Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Change history
20 December 2022
A Correction to this paper has been published: https://doi.org/10.1007/s00299-022-02964-w
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Acknowledgements
This work was supported by the China Agriculture Research System of MOF and MARA [grant number CARS-08], Guangdong Basic and Applied Basic Research Foundation [grant number 2021A1515012110] and the Agricultural Science and Technology Innovation Program (ASTIP) from Chinese Academy of Agricultural Sciences.
Funding
This work was supported by the China Agriculture Research System of MOF and MARA [grant number CARS-08], Guangdong Basic and Applied Basic Research Foundation [grant number 2021A1515012110] and the Agricultural Science and Technology Innovation Program (ASTIP) from Chinese Academy of Agricultural Sciences.
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Conceptualization: [X. G.]; Data curation: [Y. C.], [H. C.]; Formal analysis: [Y. Z.]; Funding acquisition: [L. W.], [X. G.]; Investigation: [X. C.]; Methodology: [H. C.], [X. G.]; Project administration: [X. G.]; Resources: [X. C.]; Software: [Y. C.], [Y. Z.]: Supervision: [X. G.]; Validation: [Y. C.], [L. W.]; Visualization: [Y. C.]; Writing – original draft: [Y. C.]; Writing – review & editing: [X. G.], [L. W.]. All authors read and approved the final manuscript.
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Cheng, Y., Chen, H., Zhao, Y. et al. Effect of light quality on polyphenol biosynthesis in three varieties of mung bean sprouts with different color seed coats. Plant Cell Rep 42, 253–268 (2023). https://doi.org/10.1007/s00299-022-02954-y
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DOI: https://doi.org/10.1007/s00299-022-02954-y