Abstract
Key message
Seventeen classical MaAGPs and 9 MbAGPs were identified and analyzed. MaAGP1/2/6/9/16/17, the antigens of JIM13 and LM2 antibodies are likely to be involved in banana chilling tolerance.
Classical arabinogalactan proteins (AGPs) belong to glycosylphosphatidylinositol-anchored proteins, which are proved to be involved in signaling and cell wall metabolism upon stresses. However, rare information is available on the roles of classical AGPs in low temperature (LT) tolerance. Cultivation of banana in tropical and subtropical region is seriously threatened by LT stress. In the present study, 17 classical MaAGPs and nine MbAGPs in banana A and B genome were identified and characterized, respectively. Great diversity was present among different classical MaAGP/MbAGP members while five members (AGP3/6/11/13/14) showed 100% identity between these two gene families. We further investigated different responses of classical AGPs to LT between a chilling sensitive (CS) and tolerant (CT) banana cultivars. In addition, different changes in the temporal and spatial distribution of cell wall AGP components under LTs between these two cultivars were compared using immunofluorescence labeling. Seven classical MbAGPs were upregulated by LT(s) in the CT cultivar. Classical MaAGP4/6 was induced by LT(s) in both cultivars while MaAGP1/2/9/16/17 only in the CT cultivar. Moreover, these genes showed significantly higher transcription abundance in the CT cultivar than the CS one under LT(s) except classical MaAGP4. Similar results were observed with the epitopes of JIM13 and LM2 antibodies. The antigens of these antibodies and classical MaAGP1/2/6/9/16/17 might be related to LT tolerance of banana. These results provide additional information about plant classical AGPs and their involvement in LT tolerance, as well as their potential as candidate genes to be targeted when breeding CT banana.
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Abbreviations
- AGPs:
-
Arabinogalactan proteins
- CS:
-
Chilling-sensitive
- CT:
-
Chilling-tolerant
- GPI:
-
Glycosylphosphatidylinositol
- LT:
-
Low temperature
- PASTT%:
-
The percentage of Pro, Ala, Ser, and Thr in whole protein sequence
- qPCR:
-
Quantitative real-time PCR
- MEME:
-
Multiple Em for Motif Elucidation
- MW:
-
Molecular weight
- NJ:
-
Neighbor-joining
- UTR:
-
Untranslated region
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Funding
This work was supported by the National Key Research and Development Program of China (2018YFD1000300), the Key Research and Development Program of Guangdong Province for Modern Plant Breeding (2021B0707010004, 2018B020202005), the Guangdong Province Special Fund for Modern Agriculture Industry Technology Innovation Teams (2022KJ109), the Earmarked Fund for CARS (CARS-31). The development and distribution of antibodies (except LM2 and LM14) used in the present study were supported in part by NFS grants DBI-0421683 and RCN-0090281.
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CX, XL, HC and CC conceived and designed research. JL, JM, TN, ZH, LD and ZS conducted experiments and/or prepared the materials. CX and JL analyzed the data and wrote the manuscript. All authors read and approved the manuscript.
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Liu, J., Meng, J., Chen, H. et al. Different responses of banana classical AGP genes and cell wall AGP components to low-temperature between chilling sensitive and tolerant cultivars. Plant Cell Rep 41, 1693–1706 (2022). https://doi.org/10.1007/s00299-022-02885-8
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DOI: https://doi.org/10.1007/s00299-022-02885-8