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Introgression and QTL mapping conferring resistance for Alternaria brassicae in the backcross progeny of Sinapis alba + Brassica juncea somatic hybrids

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A total of three QTLs, responsible for A. brassicae resistance were introgressed into S. alba − B. juncea introgression lines from S. alba and mapped through donor genome-specific SSR markers.

Abstract

Alternaria brassicae is a key pathogen of the Brassicaceae family causing severe blight disease to oilseed crops that leads to heavy yield losses due to lack of resistance source within cultivated Brassicas. However, the host resistance present in the Sinapis alba, an allied member of the Brassicaceae family is still unexplored precisely due to the unavailability of segregating population for Alternaria blight resistance and scarcity of donor genome-specific genetic markers. The present study was undertaken to identify quantitative trait loci governing resistance to Alternaria blight which was introgressed from S. alba to the backcross population of stable S. alba + B. juncea somatic hybrids (2n = 60; AABBSS). The second backcross population showed significant phenotypic variations for Alternaria blight ranging from immune to highly susceptible phenotype, thus suggesting quantitative nature of resistance for the disease. A subset of 154 BC2F3-4 lines was used for disease screening and genotyping with 234 S. alba genome-specific microsatellite markers. As a result of the study, twelve linkage groups were developed corresponding to 12 chromosomes of S. alba (n = 12) covering a length of 1694.02 cM. The chromosomes 5 and 11 harbored a total of 1 (Abr-01), and 2 (Abr-02, and Abr-03) QTLs detected by ICIM-ADD mapping method at LOD score values 3.7, 5.12, and 6.74, respectively. The QTLs identified during the study have a range of 5.51–10.87 percent phenotypic variations for disease resistance. To the best of our knowledge, this is the first report of QTLs introgression for A. brassicae resistance in cultivated Brassica from an allied member of Brassicaceae.

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References

  • Aneja JK, Agnihotri A (2013) Alternaria blight of oilseed Brassicas: epidemiology and disease control strategies with special reference to use of biotechnological approaching for attaining host resistance. J Oilseed Brassica 4(1):1–10

    Google Scholar 

  • Brun H, Plessis J, Renard M (1987) Resistance of some crucifers to Alternaria brassicae (Berk.) Sacc. Proc. Seventh Int. Rapeseed Congr, Poznan, Poland, pp 1222–1227

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    Article  CAS  Google Scholar 

  • Conn KL, Tewari JP (1986) Hypersensitive reaction induced by Alternaria brassicae in Eruca sativa, an oil-yielding crucifer. Can J Plant Path 8:348

    Google Scholar 

  • Conn KL, Tewari JP, Dahiya JS (1988) Resistance to Alternaria brassicae and phytoalexins-elicitation in rapeseed and other crucifers. Plant Sci 56:21–25. https://doi.org/10.1016/0168-9452(88)90180-X

    Article  CAS  Google Scholar 

  • Gaikwad K, Kirti PB, Sharma A, Prakash S, Chopra VL (1996) Cytogenetical and molecular investigations on somatic hybrids of Sinapis alba and Brassica juncea and their backcross progeny. Plant Breeding 115(6):480–483. https://doi.org/10.1111/j.1439-0523.1996.Tb00961.x

    Article  CAS  Google Scholar 

  • Giri P, Taj G, Kumar A (2013) Comparison of artificial inoculation methods for studying pathogenesis of Alternaria brassicae (Berk.) Sacc on Brassica juncea (L.) Czern. (Indian mustard). African J Biotechnol 12(18):2422–2426. https://doi.org/10.5897/AJB12.2803

    Article  CAS  Google Scholar 

  • Hansen LH, Earle ED (1997) Somatic hybrids between Brassica oleracea (L.) and Sinapis alba (L.) with re-sistance to Alternaria brassicae (Berk.) Sacc. Theor Appl Genet 94:1078–1085. https://doi.org/10.1007/s001220050518

    Article  Google Scholar 

  • Javidfar F, Cheng B (2013) Construction of a genetic linkage map and QTL analysis of erucic acid content and glucosinolate components in yellow mustard (Sinapis alba L.). BMC Plant Biol 13:142. https://doi.org/10.1186/1471-2229-13-142

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kirti PB, Mohapatra T, Khanna H, Prakash S, Chopra VL (1995) Diplotaxis catholica + Brassica juncea somatic hybrids: molecular and cytogenetic characterization. Plant Cell Rep 14:593–597

    Article  CAS  Google Scholar 

  • Kolte SJ (1985) Diseases of annual edible oilseed crops. Rapeseed-mustard and sesame diseases, vol II. CRC Press Inc., Boca Raton, Florida, p 135

    Google Scholar 

  • Kolte SJ (2002) Diseases and their management in oilseed crops new paradigm in oilseeds and oils: research and development needs. In: Rai M, Singh H, Hedge DM (eds) Indian society of oilseeds research. Hyderabad, India, pp 244–252

    Google Scholar 

  • Kolte SJ, Awasthi RP (1987) Assessment of yield losses due to Alternaria blight in rapeseed and mustard. Indian Phytopathology 40:209–211

    Google Scholar 

  • Krishnia SK, Saharan GS, Singh D (2000) Genetics of Alternaria blight resistance in inter and intraspecific crosses of Brassica juncea and Brassica carinata. Annals Biol 16(2):211–216

    Google Scholar 

  • Kumari P, Bhat SR (2019) Allohexaploid H2 (IC0626000; INGR18031), an Indian mustard (Brassica juncea) germplasm with heat tolerance, resistant to Alternaria brassicae. Indian J Plant Genet Resourc 32:439

    Google Scholar 

  • Kumari P, Singh KP (2019) Characterization of stable somatic hybrids of Sinapis alba and Brassica juncea for Alternaria blight, Sclerotinia sclerotiorum resistance and heat tolerance. Indian Res J Ext Educt 19(2):99–103

    Google Scholar 

  • Kumari P, Bisht DS, Bhat SR (2018) Stable, fertile somatic hybrids between Sinapis alba and Brassica juncea show resistance to Alternaria brassicae and heat stress. Plant Cell Tiss Organ Cult 133:77–86. https://doi.org/10.1007/s11240-017-1362-9

    Article  Google Scholar 

  • Kumari P, Singh KP, Rai PK (2020a) Draft genome of multiple resistance donor plant Sinapis alba: an insight into SSRs, annotations and phylogenetics. PLoS One 15(4):e0231002. https://doi.org/10.1371/journal.pone.0231002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kumari P, Singh KP, Bisht D, Kumar S (2020b) Somatic hybrids of Sinapis alba + Brassica juncea: study of backcross progenies for morphological variations, chromosome constitution and reaction to Alternaria brassicae. Euphytica 216:93. https://doi.org/10.1007/s10681-020-02629-3

    Article  CAS  Google Scholar 

  • Mandal S, Rajarammohan S, Kaur J (2018) Alternaria brassicae interactions with the model Brassicaceae member Arabidopsis thaliana closely resembles those with Mustard (Brassica juncea). Physiol Mol Biol Plants 24:51–59. https://doi.org/10.1007/s12298-017-0486-z

    Article  CAS  PubMed  Google Scholar 

  • Medhi BN (1985) Intermating and mutagenesis for release of genetic variability in Indian mustard (Brassica juncea L Czern and Coss) PhD thesis, Punjab Agriculture University, Ludhiana

  • Meena PD, Awasthi RP, Chattopadhyay C, Kolte SJ, Kumar A (2010) Alternaria blight: a chronic disease in rapeseed-mustard. J Oilseed Brassica 1(1):1–11

    Google Scholar 

  • Meena PD, Rani A, Meena R, Sharma P, Gupta R, Chowdappa P (2012) Aggressiveness, diversity and distribution of Alternaria brassicae isolates infecting oilseed Brassica in India. African J Microbiol Res 6(24):5249–5258. https://doi.org/10.5897/AJMR12.705

    Article  Google Scholar 

  • Meena PD, Jambhulkar SJ, Gupta R, Meena HS, Singh D (2016) Rapid screening technique for alternaria blight resistance in indian mustard (Brassica juncea L.) using cotyledonary leaf method. J Plant Pathol 98(3):463–469

    Google Scholar 

  • Mehta N, Sangwan MS, Srivastava MP, Kumar R (2002) Survival of Alternaria brassicae causing Alternaria blight in rapeseed-mustard. J Mycol Pl Pathol 32:64–67

    Google Scholar 

  • Meng L, Li H, Zhang L, Wang J (2015) QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. The Crop Journal 3(3):269–283. https://doi.org/10.1016/j.cj.2015.01.001

    Article  Google Scholar 

  • National Institute of Agricultural Botany (NIAB) (1985) Diseases of oilseed rape and fodder brassicas. United Kingdom, Cambridge

    Google Scholar 

  • Parada RY, Sakuna E, Mori N, Oka K, Egusa M, Kodoma M, Otani H (2008) Alternaria brassicae produces a host-specific protein toxin from germinating spores on host leaves. Phytopathology 98:458–463. https://doi.org/10.1094/PHYTO-98-4-0458

    Article  CAS  PubMed  Google Scholar 

  • Primard C, Vedel F, Mathieu C, Pelletier G, Chevre AM (1988) Interspecific somatic hybridization between Brassica napus and Brassica hirta (Sinapis alba L.). Theor Appl Genet 75:546–552

    Article  CAS  Google Scholar 

  • Rajarammohan S, Kumar A, Gupta V, Pental D, Pradhan AK, Kaur J (2017) Genetic architecture of resistance to Alternaria brassicae in Arabidopsis thaliana: QTL mapping reveals two major resistance-conferring loci. Front Plant Sci 8:260. https://doi.org/10.3389/fpls.2017.00260

    Article  PubMed  PubMed Central  Google Scholar 

  • Rajarammohan S, Pradhan AK, Pental D, Kaur J (2018) Genome-wide association mapping in Arabidopsis identifies novel genes underlying quantitative disease resistance to Alternaria brassicae. Mol Plant Pathol 19(7):1719–1732. https://doi.org/10.1111/mpp.12654

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sharma G, Kumar VD, Haque A, Bhat SR, Prakash S, Chopra VL (2002) Brassica coenospecies: a rich reser-voir for genetic resistance to leaf spot caused by Alternaria brassicae. Euphytica 125:411–417. https://doi.org/10.1023/A:1016050631673

    Article  Google Scholar 

  • Singh KP, Kumari P, Rai PK (2021) Current status of the disease-resistant gene(s)/QTLs, and strategies for improvement in Brassica juncea. Front Plant Sci 12:617405. https://doi.org/10.3389/fpls.2021.617405

    Article  PubMed  PubMed Central  Google Scholar 

  • Tanksley SD, Nelson JC (1996) Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet 92:191–203. https://doi.org/10.1007/BF00223376

    Article  CAS  PubMed  Google Scholar 

  • Tewari JP (1991) Resistance to Alternaria brassicae in crucifers. Bull-OILS-SROP 14(6):154–161

    Google Scholar 

  • Tewari JP, Conn KL (1993) Reactions of some wild crucifers to Alternaria brassicae. Bulletin-OILS-SROP 16:53–58

    Google Scholar 

  • Tripathi NN, Kaushik CD (1984) Studies on the survival of Alternaria brassicae the causal organism of leaf spot of rapeseed and mustard. Madras Agric J 71:237–241

    Google Scholar 

  • Tripathi NN, Kaushik CD, Yadav TP, Yadav AK (1980) Alternaria leaf spot resistance in raya. Haryana Agric Univ J Res 10:166–168

    Google Scholar 

  • Verma PR, Saharan GS (1994) Monograph on Alternaria diseases of crucifers. Saskatoon, Agriculture Canada Research Station, p 162 (Technical Bulletin No. 1994-6E)

    Google Scholar 

  • Wight WD, Kim KH, Lawrence CB, Walton JD (2009) Biosynthesis and role in virulence of the histone deacetylase inhibitor depudecin from Alternaria brassicicola. MPMI 22(10):1258–1267. https://doi.org/10.1094/MPMI-22-10-1258

    Article  CAS  PubMed  Google Scholar 

  • Zhang FL, Xu JB, Takahata Y (1996) Inheritance of resistance to black leaf spot (Alternaria brassicae) in Chinese cabbage. Cruciferae Newsl 18:134–135

    Google Scholar 

  • Zhang X, Liu T, Duan M, Song J, Li X (2016) De novo transcriptome analysis of Sinapis alba in revealing the glucosinolate and phytochelatin pathways. Front Plant Sci 7:259. https://doi.org/10.3389/fpls.2016.00259

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhu J, Spanier A (1991) Resistance sources to Phoma lingam and Alternaria brassicae. Eucarpia Crucifarae Newsl 14:143

    Google Scholar 

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Funding

(1) Preetesh Kumari: (File No. YSS/2015/001849), Science and Engineering Research Board, Ministry of Science & Technology, New Delhi, India for funding the research work; (File No. SR/WOS-A/LS-373/2018), Department of Science and Technology, Ministry of Science and Technology, New Delhi, Govt. of India, India for fellowship; (2) Kaushal Pratap Singh, received Research Associateship [File No. 19/1247(0001)/19-EMR-I] from HRDG-Council of Scientific and Industrial Research, New Delhi.

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  1. Kaushal Pratap Singh and Preetesh Kumari co-first authors.

Authors and Affiliations

  1. ICAR-Directorate of Rapeseed Mustard Research, Sewar, Bharatpur, 321303, India

    Kaushal Pratap Singh

  2. Genetics Division, ICAR-Indian Agriculture Research Institute, Pusa Campus, New Delhi, 110012, India

    Preetesh Kumari & Devendra Kumar Yadava

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  1. Kaushal Pratap Singh
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  3. Devendra Kumar Yadava
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Contributions

KPS: Developed S. alba-specific SSRs primers, QTL mapping and analyzed data, disease screening for Alternaria blight disease, draft, and finalized manuscript; PK: Conception and design of work, developed plant material, validated markers, genotyping, raised funds of the study, editing, and finalization of the manuscript; DKY: Provided field facilities for population development and disease screening, editing and finalization of the manuscript. All authors read and approved the final manuscript for submission.

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Correspondence to Preetesh Kumari.

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Singh, K.P., Kumari, P. & Yadava, D.K. Introgression and QTL mapping conferring resistance for Alternaria brassicae in the backcross progeny of Sinapis alba + Brassica juncea somatic hybrids. Plant Cell Rep 40, 2409–2419 (2021). https://doi.org/10.1007/s00299-021-02785-3

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