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Pyramiding ascorbate–glutathione pathway in Lycopersicum esculentum confers tolerance to drought and salinity stress

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Abstract

Key message

Stacking Glutathione–Ascorbate pathway genes (PgSOD, PgAPX, PgGR, PgDHAR and PgMDHAR) under stress inducible promoter RD29A imparts significant tolerance to drought and salinity stress in Solanum lycopersicum.

Abstract

Although the exposure of plants to different environmental stresses results in overproduction of reactive oxygen species (ROS), many plants have developed some unique systems to alleviate the ROS production and mitigate its deleterious effect. One of the key pathways that gets activated in plants is ascorbate glutathione (AsA-GSH) pathway. To demonstrate the effect of this pathway in tomato, we developed the AsA-GSH overexpression lines by stacking the genes of the AsA-GSH pathway genes isolated from Pennisetum glaucoma (Pg) including PgSOD, PgAPX, PgGR, PgDHAR and PgMDHAR under stress inducible promoter RD29A. The overexpression lines have an improved germination and seedling growth with concomitant elevation in the survival rate. The exposure of transgenic seedlings to varying stress regiments exhibited escalation in the antioxidant enzyme activity and lesser membrane damage as reflected by decreased electrolytic leakage and little accumulation of malondialdehyde and H2O2. Furthermore, the transgenic lines accumulated high levels of osmoprotectants with increase in the relative water content. The increased photosynthetic activity and enhanced gaseous exchange parameters further confirmed the enhanced tolerance of AsA-GSH overexpression lines. We concluded that pyramiding of AsA-GSH pathway genes is an effective strategy for developing stress resistant crops.

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Acknowledgements

Authors acknowledge the financial support by Department of Biotechnology (DBT), New Delhi under the grant number BT/PR4865/PBD/16/972/2012.

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Authors and Affiliations

  1. Plant Molecular Biology Laboratory, Department of Botany, University of Kashmir, Srinagar, Kashmir, 190006, India

    Vaseem Raja, Umer Majeed Wani, Nelofer Jan & Riffat John

  2. Department of Biotechnology, University of Kashmir, Srinagar, 190006, India

    Umer Majeed Wani & Zubair Ahmad Wani

  3. International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 1100067, India

    Chandrasekhar Kottakota, Malireddy K. Reddy & Tanushri Kaul

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  1. Vaseem Raja
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  2. Umer Majeed Wani
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Contributions

RJ and TK have conceived the study and supervised the experimental work, MKR have reviewed the work performed by CK, CK have performed the cloning part. VR, UMW and ZAW have performed the experiments and drafted the manuscript. NJ have assisted while performing the experimental work and drafting the paper.

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Correspondence to Riffat John.

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Communicated by M.Nasir Khan.

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Raja, V., Wani, U.M., Wani, Z.A. et al. Pyramiding ascorbate–glutathione pathway in Lycopersicum esculentum confers tolerance to drought and salinity stress. Plant Cell Rep 41, 619–637 (2022). https://doi.org/10.1007/s00299-021-02764-8

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