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Overexpressing heat-shock protein OsHSP50.2 improves drought tolerance in rice

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Abstract

Key message

OsHSP50.2, an HSP90 family gene up-regulated by heat and osmotic stress treatments, positively regulates drought stress tolerance probably by modulating ROS homeostasis and osmotic adjustment in rice.

Abstract

Heat-shock proteins (HSPs) serve as molecular chaperones for a variety of client proteins in abiotic stress response and play pivotal roles in protecting plants against stress, but the molecular mechanism remains largely unknown. Here, we report an HSP90 family gene, OsHSP50.2, which acts as a positive regulator in drought stress tolerance in rice (Oryza sativa). OsHSP50.2 was ubiquitously expressed and its transcript level was up-regulated by heat and osmotic stress treatments. Overexpression of OsHSP50.2 in rice reduced water loss and enhanced the transgenic plant tolerance to drought and osmotic stresses. The OsHSP50.2-overexpressing plants exhibited significantly lower levels of electrolyte leakage and malondialdehyde (MDA) and less decrease of chlorophyll than wild-type plants under drought stress. Moreover, the OsHSP50.2-overexpressing plants had significantly higher SOD activity under drought stress compared with the wild type. These results imply that OsHSP50.2 positively regulates drought stress tolerance in rice, probably through the modulation of reactive oxygen species (ROS) homeostasis. Additionally, the OsHSP50.2-overexpressing plants accumulated significantly higher content of proline than the wild type under drought stress, which contributes to the improved protection ability from drought stress damage via osmotic adjustment. Our findings reveal that OsHSP50.2 plays a crucial role in drought stress response, and it may possess high potential usefulness in drought tolerance improvement of rice.

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Abbreviations

HSF:

Heat-shock factor

HSP:

Heat-shock protein

MDA:

Malondialdehyde

MS:

Murashige and Skoog

OE:

Overexpression

PEG:

Polyethylene glycol

qRT-PCR:

Quantitative reverse transcription-polymerase chain reaction

ROS:

Reactive oxygen species

RT:

Reverse transcription

SOD:

Superoxide dismutase

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant Nos. 31401943 and 31671628), the Research Foundation of Education Bureau of Hunan Province, China (Grant No. 14C0453) and Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University.

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Authors and Affiliations

  1. Institute of Ecological Landscape Restoration, Hunan University of Science and Technology, Taoyuan Rd., Xiangtan, 411201, Hunan, China

    Jianhua Xiang, Wei Hu, Yanci Xiang & Jieming Wang

  2. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China

    Xinbo Chen

  3. School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, China

    Mingli Yan

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  1. Jianhua Xiang
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  2. Xinbo Chen
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  3. Wei Hu
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  4. Yanci Xiang
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Correspondence to Jianhua Xiang.

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Communicated by Leena Tripathi.

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Xiang, J., Chen, X., Hu, W. et al. Overexpressing heat-shock protein OsHSP50.2 improves drought tolerance in rice. Plant Cell Rep 37, 1585–1595 (2018). https://doi.org/10.1007/s00299-018-2331-4

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