Plant Cell Reports

, Volume 37, Issue 6, pp 933–946 | Cite as

A guanine insert in OsBBS1 leads to early leaf senescence and salt stress sensitivity in rice (Oryza sativa L.)

  • Dong-Dong Zeng
  • Cheng-Cong Yang
  • Ran Qin
  • Md. Alamin
  • Er-Kui Yue
  • Xiao-Li Jin
  • Chun-Hai Shi
Original Article


Key message

A rice receptor-like kinase gene OSBBS1/OsRLCK109 was identified; this gene played vital roles in leaf senescence and the salt stress response.


Early leaf senescence can cause negative effects on rice yield, but the underlying molecular regulation is not fully understood. bilateral blade senescence 1 (bbs1), an early leaf senescence mutant with a premature senescence phenotype that occurs mainly performing at the leaf margins, was isolated from a rice mutant population generated by ethylmethane sulfonate (EMS) treatment. The mutant showed premature leaf senescence beginning at the tillering stage and exhibited severe symptoms at the late grain-filling stage. bbs1 showed accelerated dark-induced leaf senescence. The OsBBS1 gene was cloned by a map-based cloning strategy, and a guanine (G) insertion was found in the first exon of LOC_Os03g24930. This gene encodes a receptor-like cytoplasmic kinase and was named OsRLCK109 in a previous study. Transgenic LOC_Os03g24930 knockout plants generated by a CRISPR/Cas9 strategy exhibited similar early leaf senescence phenotypes as did the bbs1 mutant, which confirmed that LOC_Os03g24930 was the OsBBS1 gene. OsBBS1/OsRLCK109 was expressed in all detected tissues and was predominantly expressed in the main vein region of mature leaves. The expression of OsBBS1 could be greatly induced by salt stress, and the bbs1 mutant exhibited hypersensitivity to salt stress. In conclusion, this is the first identification of OsRLCKs participating in leaf senescence and playing critical roles in the salt stress response in rice (Oryza sativa L.).


Oryza sativa L. Early leaf senescence Receptor-like cytoplasmic kinases OsBBS1 Salt sensitive 



Base pair








Reverse transcription-quantitative polymerase chain reaction


Receptor-like kinase


Receptor-like cytoplasmic kinase


Reactive oxygen species


Superoxide dismutase


Wild type



We thank Professor Liu Yaoguang for providing the CRISPR/Cas9 system and Professor Wu Liang for the assistance during vector construction. This work was supported by the Science and Technology Office of Zhejiang Province (2012C12901-2, 2016C32085 and 2016C02050-6).

Author contribution statement

ZDD performed the main experiments and wrote the manuscript. SCH designed the experiment, and edited the final manuscript. YCC and QR measured the physiological indices. AM, YEK and JXL performed the vector constructions and DNA sequencing. All authors have read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2018_2280_MOESM1_ESM.doc (25.1 mb)
Supplementary material 1 (DOC 25728 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Dong-Dong Zeng
    • 1
  • Cheng-Cong Yang
    • 1
  • Ran Qin
    • 1
  • Md. Alamin
    • 1
  • Er-Kui Yue
    • 1
  • Xiao-Li Jin
    • 1
  • Chun-Hai Shi
    • 1
  1. 1.Department of AgronomyZhejiang UniversityHangzhouChina

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