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Isolation and functional characterization of a cold responsive phosphatidylinositol transfer-associated protein, ZmSEC14p, from maize (Zea may L.)

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Abstract

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A Sec14-like protein, ZmSEC14p , from maize was structurally analyzed and functionally tested. Overexpression of ZmSEC14p in transgenic Arabidopsis conferred tolerance to cold stress.

Abstract

Sec14-like proteins are involved in essential biological processes, such as phospholipid metabolism, signal transduction, membrane trafficking, and stress response. Here, we reported a phosphatidylinositol transfer-associated protein, ZmSEC14p (accession no. KT932998), isolated from a cold-tolerant maize inbred line using the cDNA-AFLP approach and RACE-PCR method. Full-length cDNA that consisted of a single open reading frame (ORF) encoded a putative polypeptide of 295 amino acids. The ZmSEC14p protein was mainly localized in the nucleus, and its transcript was induced by cold, salt stresses, and abscisic acid (ABA) treatment in maize leaves and roots. Overexpression of ZmSEC14p in transgenic Arabidopsis conferred tolerance to cold stress. This tolerance was primarily displayed by the increased germination rate, root length, plant survival rate, accumulation of proline, activities of antioxidant enzymes, and the reduction of oxidative damage by reactive oxygen species (ROS). ZmSEC14p overexpression regulated the expression of phosphoinositide-specific phospholipase C, which cleaves phosphatidylinositol 4,5-bisphosphate (PIP2) and generates second messengers (inositol 1,4,5-trisphosphate and 1,2-diacylglycerol) in the phosphoinositide signal transduction pathways. Moreover, up-regulation of some stress-responsive genes such as CBF3, COR6.6, and RD29B in transgenic plants under cold stress could be a possible mechanism for enhancing cold tolerance. Taken together, this study strongly suggests that ZmSEC14p plays an important role in plant tolerance to cold stress.

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Abbreviations

ABA:

Abscisic acid

ROS:

Reactive oxygen species

PIP2:

Phosphatidylinositol 4,5-bisphosphate

IP3:

Inositol 1,4,5-trisphosphate

DAG:

1,2-Diacylglycerol

COR:

Cold regulated

KIN:

Cold induced

RD:

Responsive to dehydration

CBFs:

CRT/DRE binding factor

DREBs:

DRE binding protein

DRE/CRT:

Dehydration-responsive element/C-repeat

CAMTA:

Calmodulin binding transcription activator

PIPTs:

Phosphatidylinositol transfer proteins

PtdIns:

Phosphatidylinositol

PtdCho:

Phosphatidylcholine

cDNA-AFLP:

cDNA-amplified fragment length polymorphism

RACE:

Rapid amplification of cDNA ends

WT:

Wild type

MS:

Murashige and Skoog

NBT:

Nitroblue tetrazolium

DAB:

3,3′-Diaminobenzidine

SOD:

Superoxide dismutase

POD:

Peroxidase

O2 − :

Superoxide anions

H2O2 :

Hydrogen peroxide

PLC:

Phospholipases C

PtdInsP:

Phosphatidylinositol monophosphate

PA:

Phosphatidic acid

PIPs:

Phosphoinositides

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Acknowledgments

This work was supported by the National Transgenic Crops of New Varieties Breeding Major Project-New Germplasm Combination Breeding of Cold Tolerance Transgenic Maize (20142X0800305B).

Conflict of interest

The authors have declared that no competing interests exist.

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Authors and Affiliations

  1. College of Plant Science, Jilin University, Changchun, 130062, China

    Xiaoyu Wang, Xiaohui Shan, Chunmei Xue, Ying Wu, Shengzhong Su, Shipeng Li, Hongkui Liu, Yuan Jiang, Yanfei Zhang & Yaping Yuan

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  1. Xiaoyu Wang
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  2. Xiaohui Shan
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  3. Chunmei Xue
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Correspondence to Yaping Yuan.

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Communicated by C-H Dong.

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Wang, X., Shan, X., Xue, C. et al. Isolation and functional characterization of a cold responsive phosphatidylinositol transfer-associated protein, ZmSEC14p, from maize (Zea may L.). Plant Cell Rep 35, 1671–1686 (2016). https://doi.org/10.1007/s00299-016-1980-4

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