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Identification of a novel gene (Hsdr4) involved in water-stress tolerance in wild barley

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Abstract

Drought is one of the most severe stresses limiting plant growth and yield. Genes involved in water stress tolerance of wild barley (Hordeum spontaneoum), the progenitor of cultivated barley, were investigated using genotypes contrasting in their response to water stress. Gene expression profiles of water-stress tolerant vs. water-stress sensitive wild barley genotypes, under severe dehydration stress applied at the seedling stage, were compared using cDNA-AFLP analysis. Of the 1100 transcript-derived fragments (TDFs) amplified about 70 displayed differential expression between control and stress conditions. Eleven of them showed clear difference (up- or down-regulation) between tolerant and susceptible genotypes. These TDFs were isolated, sequenced and tested by RT-PCR. The differential expression of seven TDFs was confirmed by RT-PCR, and TDF-4 was selected as a promising candidate gene for water-stress tolerance. The corresponding gene, designated Hsdr4 (Hordeum spontaneum dehydration-responsive), was sequenced and the transcribed and flanking regions were determined. The deduced amino acid sequence has similarity to the rice Rho-GTPase-activating protein-like with a Sec14 p-like lipid-binding domain. Analysis of Hsdr4 promoter region that was isolated by screening a barley BAC library, revealed a new putative miniature inverted-repeat transposable element (MITE), and several potential stress-related binding sites for transcription factors (MYC, MYB, LTRE, and GT-1), suggesting a role of the Hsdr4 gene in plant tolerance to dehydration stress. Furthermore, the Hsdr4 gene was mapped using wild barley mapping population to the long arm of chromosome 3H between markers EBmac541 and EBmag705, within a region that previously was shown to affect osmotic adaptation in barley.

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Abbreviations

cDNA-AFLP:

cDNA-amplified fragment length polymorphism

TDF:

transcript-derived fragment

RT-PCR:

reverse transcription polymerase chain reaction

Q-PCR:

quantitative expression analysis by real-time PCR

UTR:

untranslated region

SNP:

single nucleotide polymorphism

QTL:

quantitative trait locus

MITE:

miniature inverted-repeat transposable element

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Acknowledgments

This work was supported by grants from the German-Israeli Cooperation Project (DIP project No. DIP-B 4.3) funded by the BMBF and supported by BMBF’s International Bureau at the DLR; The Israeli Science Foundation (grants No. 1089/04, 9030/96, 9048/99, and 9019/01), Ministry of Science and Technology, Research Networks Program in Sustainable Agriculture between France and Israel; and the financial support of the Israel Discount Bank Chair of Evolutionary Biology and the Ancell-Teicher Research Foundation for Molecular Genetics and Evolution. We wish to thank Herman Van Eck for his assistance with cDNA-AFLP analysis; Guoxiong Chen for providing the mapping population; Hanan Sela for access to the program DNA mfold; Alan Schulman for helpful discussion and suggestions.

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Authors and Affiliations

  1. Institute of Evolution, University of Haifa, Mount Carmel, Haifa, 31905, Israel

    Tatiana Suprunova, Tamar Krugman, Assaf Distelfeld, Tzion Fahima, Eviatar Nevo & Abraham Korol

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  1. Tatiana Suprunova
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  2. Tamar Krugman
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  3. Assaf Distelfeld
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  4. Tzion Fahima
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  5. Eviatar Nevo
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  6. Abraham Korol
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Corresponding author

Correspondence to Abraham Korol.

Additional information

The sequences reported in this paper have been deposited in the NCBI and dbEST databases. The accession numbers are incorporated in the text. The accession number of Hsdr4 is DQ464370. The accession numbers of the ESTs are: TDF-2, EB174194; TDF-3, EB174195; TDF-4, EB174196; TDF-5, EB174197; TDF-7, EB174198; TDF-9, EB174199; TDF-10, EB174200; TDF-11, EB174201; TDF-12, EB174202; TDF-13, EB174203; TDF-14, EB174204.

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Suprunova, T., Krugman, T., Distelfeld, A. et al. Identification of a novel gene (Hsdr4) involved in water-stress tolerance in wild barley. Plant Mol Biol 64, 17–34 (2007). https://doi.org/10.1007/s11103-006-9131-x

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