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Characterization and primary functional analysis of a bamboo NAC gene targeted by miR164b

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Abstract

Key message

PeSNAC1 , a stress-related NAC1 from Phyllostachys edulis , was characterized. Ectopic expression in Arabidopsis indicated that PeSNAC1 together with ped -miR164b participated in the regulation of organ boundaries and stress tolerance.

Abstract

NAC (NAM, ATAF1/2 and CUC2) participates in many different processes regulating plant growth, development, and stress response. A total of 125 NAC genes have been predicted in moso bamboo (Phyllostachys edulis), but their roles are poorly understood. PeSNAC1 targeted by ped-miR164b was focused for further study. The cleavage of PeSNAC1 mRNA guided by ped-miR164b was validated using RLM-5′ RACE. Tissue-specific expression analysis demonstrated that ped-miR164b had a declining trend from root, sheath, leaf, to that of stem, which was opposite to that of PeSNAC1. Transgenic Arabidopsis plants overexpressing either PeSNAC1 (OX-PeSNAC1) or, ped-miR164b (OX-ped-miR164b) driven by the CaMV35S promoter were generated. OX-ped-miR164b plants showed similar phenotype of cuc2 mutants whose growth was seriously suppressed. Compared with Col-0, sense OX-PeSNAC1 plants grew rapidly and flowered earlier, whereas antisense plants grew slowly and exhibited delayed flowering. Sense OX-PeSNAC1 plants had the greatest number of lateral roots, while antisense OX-PeSNAC1 and OX-ped-miR164b plants had fewer lateral roots than Col-0. Under NaCl and PEG6000 stresses, survival rates were higher and F v/F m values declined more slowly in sense OX-PeSNAC1 plants than in Col-0, with lower survival rates and a more rapid decrease in F v/F m values conversely observed in antisense OX-PeSNAC1 and OX-ped-miR164b plants. These findings indicated that ped-miR164b-targeted PeSNAC1 may play key roles in plant development and tolerance to salinity and drought stresses.

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Abbreviations

ATAF:

Arabidopsis transcription activation factor

BLAST:

Basic local alignment search tool

CaMV35S :

Cauliflower mosaic virus 35S

cDNA:

Complementary DNA

F v/F m :

Maximal quantum yield of PSII

ORF:

Open reading frame

CUC:

Cup-shaped cotyledon (CUC), a NAC protein

miRNA:

MicroRNA, small regulatory RNAs

NAC:

Abbreviation of NAM, ATAF1/2 and CUC2, a plant-specific TF family

NAM:

No apical meristem (NAM), a NAC protein

PCR:

Polymerase chain reaction

qPCR:

Real time quantitative PCR

RACE:

Rapid-amplification of cDNA ends

RLM-5′ RACE:

RNA ligase-mediated 5′ RACE

TF:

Transcription factor

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Acknowledgments

This work received financial support from the Special Fund for Forest Scientific Research in the Public Welfare from State Forestry Administration of China (No. 201504106) and the Sub-Project of National Science and Technology Support Plan of the Twelfth Five-Year in China (No. 2015BAD04B01 and No. 2015BAD04B03).

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Authors and Affiliations

  1. State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China

    Lili Wang, Hansheng Zhao, Dongliang Chen, Lichao Li, Huayu Sun, Yongfeng Lou & Zhimin Gao

  2. Lianyungang Academy of Agricultural Sciences, Lianyungang, 222000, China

    Lili Wang

  3. Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China

    Dongliang Chen

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  1. Lili Wang
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  2. Hansheng Zhao
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  3. Dongliang Chen
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Correspondence to Zhimin Gao.

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Communicated by B. Li.

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Wang, L., Zhao, H., Chen, D. et al. Characterization and primary functional analysis of a bamboo NAC gene targeted by miR164b. Plant Cell Rep 35, 1371–1383 (2016). https://doi.org/10.1007/s00299-016-1970-6

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