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Elucidation of salt-tolerance metabolic pathways in contrasting rice genotypes and their segregating progenies

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Abstract

Key message

Differentially expressed antioxidant enzymes, amino acids and proteins in contrasting rice genotypes, and co-location of their genes in the QTLs mapped using bi-parental population, indicated their role in salt tolerance.

Abstract

Soil salinity is a major environmental constraint limiting rice productivity. Salt-tolerant ‘CSR27’, salt-sensitive ‘MI48’and their extreme tolerant and sensitive recombinant inbred line (RIL) progenies were used for the elucidation of salt stress tolerance metabolic pathways. Salt stress-mediated biochemical and molecular changes were analyzed in the two parents along with bulked-tolerant (BT) and bulked-sensitive (BS) extreme RILs. The tolerant parent and BT RILs suffered much lower reduction in the chlorophyll as compared to their sensitive counterparts. Activities of antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD) and non-enzymatic antioxidant ascorbic acid were much higher in salt-stressed CSR27 and BT RILs than MI48 and BS RILs. Further, the tolerant lines showed significant enhancement in the levels of amino acids methionine and proline in response to salt stress in comparison to the sensitive lines. Similarly, the tolerant genotypes showed minimal reduction in cysteine content whereas sensitive genotypes showed a sharp reduction. Real time PCR analysis confirmed the induction of methionine biosynthetic pathway (MBP) enzymes cystathionine-β synthase (CbS), S-adenosyl methionine synthase (SAMS), S-adenosyl methionine decarboxylase (SAMDC) and serine hydroxymethyl transferase (SHMT) genes in tolerant lines, suggesting potential role of the MBP in conferring salt tolerance in rice variety CSR27. Proteome profiling also confirmed higher expression of SOD, POD and plastidic CbS and other proteins in the tolerant lines, whose genes were co-located in the QTL intervals for salt tolerance mapped in the RIL population. The study signifies integrated biochemical-molecular approach for identifying salt tolerance genes for genetic improvement for stress tolerant rice varieties.

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Abbreviations

ACN:

Acetonotrile

PMF:

Peptide mass fingerprint

TFA:

Trifluoroacetic acid

SOD:

Superoxide dismutase

ROS:

Reactive oxygen species

CAT:

Catalase

POD:

Peroxidase

CHAPS:

3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulphonate

IEF:

Isoelectric focusing

IPG:

Immobilized pH gradient

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Acknowledgments

We thankfully acknowledge the financial support of Indian Council of Agricultural Research under the NPTC project (NPTC/2049-3021), VR and TT are thankful to funding support by Department of Biotechnology, Ministry of Science and Technology, Government of India (DST/INT/JSPS/PROJ/10).

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    Authors and Affiliations

    1. National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India

      Pragya Mishra, Vagish Mishra, Vandna Rai & Nagendra Kumar Singh

    2. Banasthali University, Tonk, Rajasthan, India

      Pragya Mishra

    3. Plant Biotechnology Research Center, Meijo University, Nagoya, Japan

      Teruhiro Takabe

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    Communicated by M. Prasad.

    P. Mishra and V. Mishra contributed equally.

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    Mishra, P., Mishra, V., Takabe, T. et al. Elucidation of salt-tolerance metabolic pathways in contrasting rice genotypes and their segregating progenies. Plant Cell Rep 35, 1273–1286 (2016). https://doi.org/10.1007/s00299-016-1959-1

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