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Asymbiotic in vitro seed propagation of Dendrobium

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Abstract

The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro techniques for their asymbiotic seed germination. The influence of abiotic factors (such as temperature and light), methods of sterilization, composition of basal media, and supplementation with organic additives and plant growth regulators are discussed in context to achieve successful seed germination, protocorm formation, and further seedling growth and development. This review provides both a basis for the selection of optimal conditions, and a platform for the discovery of better ones, that would allow the development of new protocols and the exploration of new hypotheses for germination and conservation of Dendrobium seeds and seedlings.

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Abbreviations

2-iP:

2-Isopentenyladenine

AC:

Activated charcoal

AH:

Apple homogenate

BA:

N6-Benzyladenine

BP:

Banana pulp/paste

CW:

Coconut water

GA3 :

Gibberellic acid

NAA:

α-Naphthaleneacetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kin:

Kinetin

OMF:

Orchid mycorrhizal fungi

PGR:

Plant growth regulator

PPFD:

Photosynthetic photon flux density

TDZ:

Thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5-ylurea)

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Acknowledgments

The authors thank Dr. Meesawat Upatham (Prince of Songkla University, Thailand) for comments and opinions on an earlier version of the manuscript.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. P. O. Box 7, Miki-cho Post Office, 3011-2, Ikenobe, Kagawa, 761-0799, Japan

    Jaime A. Teixeira da Silva

  2. Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, Leninskie gory 1-12, 119234, Moscow, Russia

    Elena A. Tsavkelova

  3. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

    Tzi Bun Ng

  4. Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China

    Tzi Bun Ng

  5. Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    S. Parthibhan & M. V. Rao

  6. Research Institute of Nyíregyháza, University of Debrecen, P.O. Box 12, 4400, Nyíregyháza, Hungary

    Judit Dobránszki

  7. Department of Rural Development, Centro de Ciências Agrárias, UFSCar, Via Anhanguera, km 174, CP 153, CEP 13.600-970, Araras City, Brazil

    Jean Carlos Cardoso

  8. Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Songjun Zeng

Authors
  1. Jaime A. Teixeira da Silva
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  2. Elena A. Tsavkelova
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  3. Tzi Bun Ng
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  4. S. Parthibhan
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  5. Judit Dobránszki
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  6. Jean Carlos Cardoso
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  7. M. V. Rao
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  8. Songjun Zeng
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Corresponding authors

Correspondence to Jaime A. Teixeira da Silva, Elena A. Tsavkelova, Tzi Bun Ng, S. Parthibhan, Judit Dobránszki, Jean Carlos Cardoso, M. V. Rao or Songjun Zeng.

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Communicated by N. Stewart.

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Teixeira da Silva, J.A., Tsavkelova, E.A., Ng, T.B. et al. Asymbiotic in vitro seed propagation of Dendrobium . Plant Cell Rep 34, 1685–1706 (2015). https://doi.org/10.1007/s00299-015-1829-2

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  • DOI: https://doi.org/10.1007/s00299-015-1829-2

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