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Fruit harvesting time and corresponding morphological changes of seed integuments influence in vitro seed germination of Dendrobium nobile Lindl.

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Abstract

In vitro asymbiotic seed germination of Dendrobium nobile varied significantly with fruit harvesting time and growth medium used for culturing seeds. Seeds harvested 129 days after pollination (DAP) possessing globular shaped embryos and a discontinuous cuticle layer showed a substantially greater germination on P668 medium. Alternatively, immature seeds harvested 96 and 116 DAP displayed a significantly lower germination response on various growth media. Most of the ovules at 96 DAP are in archesporial and megaspore mother cell stages, whereas the majority of ovules are mature and fertilized at 116 DAP. Mature seeds harvested 158 DAP also germinated at a higher frequency at Stage 5 (emergence of the first leaf) after 8 weeks of culture on different growth media indicating the absence of testa imposed dormancy in this endangered epiphytic orchid.

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Abbreviations

DAP:

Days after pollination

DAPI:

4′-6-diamidino-2-phenylindole

TTC:

2, 3, 5-triphenyl-2H-tetrazolium chloride

P668:

Phytotechnology medium

MS:

Murashige and Skoog (1962) medium

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Acknowledgments

Financial support provided by the National Research Foundation (NRF), Pretoria is gratefully acknowledged. We also appreciate the prompt help provided by all the staff of Microscopy Unit (Mr Tony Bruton, Ms Shirley Mackellar, Mr Vijay Bandu, Ms Pat Joubert, Ms Priscilla Donnelly, and Ms Tutuzwa Xuma).

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  1. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    Rajeswari Vasudevan & Johannes van Staden

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  1. Rajeswari Vasudevan
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  2. Johannes van Staden
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Correspondence to Johannes van Staden.

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Vasudevan, R., van Staden, J. Fruit harvesting time and corresponding morphological changes of seed integuments influence in vitro seed germination of Dendrobium nobile Lindl.. Plant Growth Regul 60, 237–246 (2010). https://doi.org/10.1007/s10725-009-9437-1

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  • DOI: https://doi.org/10.1007/s10725-009-9437-1

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