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GRY79 encoding a putative metallo-β-lactamase-trihelix chimera is involved in chloroplast development at early seedling stage of rice

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The green - revertible yellow79 mutant resulting from a single-base mutation suggested that the GRY79 gene encoding a putative metallo-β-lactamase-trihelix chimera is involved in chloroplast development at early seedling stage of rice.

Abstract

Functional studies of metallo-β-lactamases and trihelix transcription factors in higher plants remain very sparse. In this study, we isolated the green-revertible yellow79 (gry79) mutant in rice. The mutant developed yellow-green leaves before the three-leaf stage but recovered to normal green at the sixth-leaf stage. Meanwhile, the mutant exhibited reduced level of chlorophylls and arrested development of chloroplasts in the yellow leaves. Genetic analysis suggested that the mutant phenotype was controlled by a single recessive nuclear gene on rice chromosome 2. Map-based cloning revealed that the candidate gene was Os02g33610 encoding a putative metallo-β-lactamase-trihelix chimera. In the gry79 mutant, a single-base mutation occurred in coding region of the gene, resulting in an amino acid change in the encoded protein. Furthermore, the mutant phenotype was rescued by transformation with the wild-type gene. Therefore, we have confirmed that the gry79 mutant phenotype resulted from a single-base mutation in GRY79 (Os02g33610) gene, suggesting that the gene encoding a putative metallo-β-lactamase-trihelix chimera is involved in chloroplast development at early seedling stage of rice. In addition, we considered that the gry79 mutant gene could be applicable as a leaf-color marker gene for efficient identification and elimination of false hybrids in commercial hybrid rice production.

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Acknowledgments

We specially thank Prof. Chengcai Chu from Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, for kindly providing the binary vector pCAMBIA2300. This study was financially supported by the National Natural Science Foundation of China (31071402, 31171533, 31471473, and 91335107).

Conflict of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China

    Chunmei Wan, Chunmei Li, Xiaozhi Ma, Yang Wang, Changhui Sun, Rui Huang, Ping Zhong, Zhiyan Gao, Dan Chen, Zhengjun Xu, Jianqing Zhu, Xiaoling Gao, Pingrong Wang & Xiaojian Deng

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  1. Chunmei Wan
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Correspondence to Pingrong Wang or Xiaojian Deng.

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Communicated by K. Chong.

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299_2015_1792_MOESM1_ESM.doc

Supplemental Fig. S1 Alignment of the deduced amino acid sequence of GRY79 and its homologs. Identical residues were boxed in black, similar residues (≥75 % identical) were highlighted in gray. The black underline indicates the putative chloroplast-targeting sequence of 36 amino acid residues at its N terminus. The red and blue underlines indicate the putative metallo-β-lactamase domain of 202 amino acid residues at its N terminus and the putative trihelix DNA binding domain of 66 amino acid residues at its C terminus, respectively. The red arrowhead indicates mutational site (from serine (S) to phenylalanine (F) at position 387) of the gry79 mutant. Accession numbers for the respective protein sequences are as Fig. 4. (DOC 263 kb)

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Wan, C., Li, C., Ma, X. et al. GRY79 encoding a putative metallo-β-lactamase-trihelix chimera is involved in chloroplast development at early seedling stage of rice. Plant Cell Rep 34, 1353–1363 (2015). https://doi.org/10.1007/s00299-015-1792-y

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