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Isolation and characterization of a J domain protein that interacts with ARC1 from ornamental kale (Brassica oleracea var. acephala)

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Abstract

Key message

A novel J domain protein, JDP1, was isolated from ornamental kale. The C-terminus of JDP1 specifically interacted with ARC1, which has a conserved role in self-incompatibility signaling.

Abstract

Armadillo (ARM)-repeat containing 1 (ARC1) plays a conserved role in self-incompatibility signaling across the Brassicaceae and functions downstream of the S-locus receptor kinase. Here, we identified a J domain protein 1 (JDP1) that interacts with ARC1 using a yeast two-hybrid screen against a stigma cDNA library from ornamental kale (Brassica oleracea var. acephala). JDP1, a 38.4-kDa protein with 344 amino acids, is a member of the Hsp40 family. Fragment JDP157–344, originally isolated from a yeast two-hybrid cDNA library, interacted specifically with ARC1 in yeast two-hybrid assays. The N-terminus of JDP1 (JDP11–68) contains a J domain, and the C-terminus of JDP1 (JDP169–344) contains an X domain of unknown function. However, JDP169–344 was required and sufficient for interaction with ARC1 in yeast two-hybrid assays and in vitro binding assays. Moreover, JDP169–344 regulated the trafficking of ARC1 from the cytoplasm to the plasma membrane by interacting with ARC1 in Arabidopsis mesophyll protoplasts. Finally, Tyr8 in the JDP1 N-terminal region was identified to be the specific site for regulating the interaction between JDP1 and BoARC1 in yeast two-hybrid assays. Possible roles of JDP1 as an interactor with ARC1 in Brassica are discussed.

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Abbreviations

ARC1:

ARM-repeat containing 1

AtPUB14:

Arabidopsis thaliana PLANT U-BOX 14

AtPUB17:

Arabidopsis thaliana PLANT U-BOX 17

CFP:

Cyan fluorescent protein

GFP:

Green fluorescent protein

HPD:

Histidine–proline–aspartic acid

Hsp40:

Heat shock protein 40

Hsp70:

Heat shock protein 70

JDP1:

J Domain protein 1

MBP:

Maltose binding protein

MLPK:

M-Locus protein kinase

PUB:

PLANT U-BOX protein

RT-PCR:

Reverse transcriptase PCR

SCR:

S-Locus cysteine-rich

SI:

Self-incompatibility

SLG:

S-Locus glycoprotein

SP11:

S-Locus protein 11

SRK:

S-Locus receptor kinase

X domain:

DnaJ-X domain

YFP:

Yellow fluorescent protein

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Acknowledgments

We are grateful to Dr. Beth E. Hazen for improving the English of this manuscript. We thank Dr. Hong-Quan Yang (Shanghai Jiao Tong University) for helpful discussions and suggestions and Dr. Daphne R. Goring (University of Toronto) for providing BnARC1 cDNA. This work was supported by the Fundamental Research Funds for the Central Universities (No. DL13CA13), the National Natural Science Foundation of China (No. 30900115; 31070275) and the National Fundamental Fund of Personnel Training of China (No. J1210053).

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The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. College of Life Sciences, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China

      Xingguo Lan, Jia Yang, Mingming Cao, Yanhong Wang & Yuhua Li

    2. Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo, 113-8657, Japan

      Saneyuki Kawabata

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    1. Xingguo Lan
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    Correspondence to Yuhua Li.

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    Communicated by X. S. Zhang.

    X. Lan and J. Yang contributed equally to this work.

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    Lan, X., Yang, J., Cao, M. et al. Isolation and characterization of a J domain protein that interacts with ARC1 from ornamental kale (Brassica oleracea var. acephala). Plant Cell Rep 34, 817–829 (2015). https://doi.org/10.1007/s00299-015-1744-6

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