Abstract
Key message
An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE.
Abstract
Cassava bacterial blight, a disease caused by the gram-negative bacterium Xanthomonas axonopodis pv. manihotis (Xam), is a major limitation to cassava production worldwide and especially in developing countries. The molecular mechanisms underlying cassava susceptibility to Xam are currently unknown. To identify host genes and pathways leading to plant susceptibility, we analyzed the transcriptomic responses occurring in cassava plants challenged with either the non-pathogenic Xam strain ORST4, or strain ORST4(TALE1 Xam ) which is pathogenic due to the major virulence transcription activator like effector TALE1 Xam . Both strains triggered similar responses, i.e., induction of genes related to photosynthesis and phenylpropanoid biosynthesis, and repression of genes related to jasmonic acid signaling. Finally, to search for TALE1 Xam virulence targets, we scanned the list of cassava genes induced upon inoculation of ORST4(TALE1 Xam ) for candidates harboring a predicted TALE1 Xam effector binding element in their promoter. Among the six genes identified as potential candidate targets of TALE1 Xam a gene coding for a heat shock transcription factor stands out as the best candidate based on their induction in presence of TALE1 Xam and contain a sequence putatively recognized by TALE1 Xam .
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Acknowledgments
AMB was financially supported by a PhD fellowship from DFS, IRD, Sibaghe-UM2 (France) and Ministerio de Agricultura y Desarrollo Rural (Colombia). Funding for BS, AB, CL come from Action Ecos Nord C11A02 and Ministerio de Agricultura y Desarrollo Rural (Colombia). Funding for ALPQ comes from the European Union through Erasmus Mundus Action 2 PRECIoSA Grant Agreement nr. 2012–2646/001–001–EMA2.
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Communicated by Emmanuel Guiderdoni.
A. Muñoz-Bodnar and A. L. Perez-Quintero authors contributed equally to this work.
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299_2014_1667_MOESM1_ESM.tif
Supplementary Fig. 1. Bar plots showing the expression profiles of genes grouped in different clusters using k means. Numbers on top of the bars are the mean log FC for each cluster in the respective treatment (TIFF 16362 kb)
299_2014_1667_MOESM2_ESM.tif
Supplementary Fig. 2. Experimental validation of genes induced specifically by “+TALE1 Xam ”. Relative expression values were obtained by comparing each time point with the controls and using the 18S RNA as a reference gene, values in the graph correspond to (2−ΔCt 0dpi)/(2−ΔCt X dpi), where ΔCt = Ctgene − Ct18S. FPKM values for each gene are shown as a comparison (TIFF 2316 kb)
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Muñoz-Bodnar, A., Perez-Quintero, A.L., Gomez-Cano, F. et al. RNAseq analysis of cassava reveals similar plant responses upon infection with pathogenic and non-pathogenic strains of Xanthomonas axonopodis pv. manihotis . Plant Cell Rep 33, 1901–1912 (2014). https://doi.org/10.1007/s00299-014-1667-7
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DOI: https://doi.org/10.1007/s00299-014-1667-7