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Application of T-DNA activation tagging to identify glutamate receptor-like genes that enhance drought tolerance in plants

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Abstract

Key message

A high-quality rice activation tagging population has been developed and screened for drought-tolerant lines using various water stress assays. One drought-tolerant line activated two rice glutamate receptor-like genes. Transgenic overexpression of the rice glutamate receptor-like genes conferred drought tolerance to rice and Arabidopsis.

Abstract

Rice (Oryza sativa) is a multi-billion dollar crop grown in more than one hundred countries, as well as a useful functional genetic tool for trait discovery. We have developed a population of more than 200,000 activation-tagged rice lines for use in forward genetic screens to identify genes that improve drought tolerance and other traits that improve yield and agronomic productivity. The population has an expected coverage of more than 90 % of rice genes. About 80 % of the lines have a single T-DNA insertion locus and this molecular feature simplifies gene identification. One of the lines identified in our screens, AH01486, exhibits improved drought tolerance. The AH01486 T-DNA locus is located in a region with two glutamate receptor-like genes. Constitutive overexpression of either glutamate receptor-like gene significantly enhances the drought tolerance of rice and Arabidopsis, thus revealing a novel function of this important gene family in plant biology.

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Abbreviations

ATL:

Activation tagging line

CaMV:

Cauliflower mosaic virus

CTAB:

Cetyltrimethyl ammonium bromide

CDS:

Coding sequence

DsRed:

Discosoma red fluorescent protein

GLR:

Glutamate receptor-like

RNAi:

RNA interference

RT-PCR:

Reverse-transcription polymerase chain reaction

T-DNA:

Transfer DNA

UTR:

Untranslated region

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Acknowledgments

We thank Li Xin, Guimin Zhang, Jing Zhang, Chunxia Liu, Guangwu Chen, Xiaocui Huang, Yingbin Chen, Cong Li, Chengfeng Du, Huanming Sun, Xiuping Meng, Qiaoyan Pan, Guowen Zhang, Tom Greene, Bo Shen, and James Zhou, et al. for their contributions and supports to the research program. Thanks to the employees of Beijing Kaituo DNA Biotech Research Center, Co., Ltd., Beijing Weiming Kaituo Crop Co., Ltd., and DuPont Pioneer for their various supports. Thanks also to Binbin Lei and Shufen Li for their critical review of the manuscript. This research was supported by DuPont Pioneer ABT collaboration program.

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Authors and Affiliations

  1. Beijing Kaituo DNA Biotech Research Center, Co., Ltd., Beijing, 102206, China

    Guihua Lu, Xiping Wang, Junhua Liu, Kun Yu, Yang Gao, Haiyan Liu, Changgui Wang, Wei Wang, Guokui Wang, Min Liu, Guanfan Mao, Binfeng Li, Jianying Qin, Mian Xia, Junli Zhou, Jingmei Liu, Shuqin Jiang & Jinteng Cui

  2. DuPont Pioneer Agriculture Biotechnology, Johnston, Iowa, IA, 50131, USA

    Hua Mo, Shoba Sivasankar, Marc C. Albertsen & Michael W. Lassner

  3. DuPont Pioneer Agriculture Biotechnology, Wilmington, Delaware, DE, 19880, USA

    Nobuhiro Nagasawa, Hajime Sakai, Barbara J. Mazur & Richard M. Broglie

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  1. Guihua Lu
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Correspondence to Richard M. Broglie.

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Communicated by K. Chong.

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Lu, G., Wang, X., Liu, J. et al. Application of T-DNA activation tagging to identify glutamate receptor-like genes that enhance drought tolerance in plants. Plant Cell Rep 33, 617–631 (2014). https://doi.org/10.1007/s00299-014-1586-7

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