Abstract
To investigate the regulation of 14-3-3 proteins in banana (Musa acuminata L. AAA group, cv. Brazilian) fruit postharvest ripening, four cDNAs encoding 14-3-3 proteins were isolated from banana and designated as Ma-14-3-3a, Ma-14-3-3c, Ma-14-3-3e, and Ma-14-3-3i, respectively. Amino acid sequence alignment showed that the four 14-3-3 proteins shared a highly conserved core structure and variable C-terminal as well as N-terminal regions with 14-3-3 proteins from other plant species. Phylogenetic analysis revealed that the four 14-3-3 genes belong to the non-ε groups. They were differentially and specifically expressed in various tissues. Real-time RT-PCR analysis indicated that these four genes function differentially during banana fruit postharvest ripening. Three genes, Ma-14-3-3a, Ma-14-3-3c, and Ma-14-3-3e, were significantly induced by exogenous ethylene treatment. However, gene function differed in naturally ripened fruits. Ethylene could induce Ma-14-3-3c expression during postharvest ripening, but expression patterns of Ma-14-3-3a and Ma-14-3-3e suggest that these two genes appear to be involved in regulating ethylene biosynthesis during fruit ripening. No obvious relationship emerged between Ma-14-3-3i expression in naturally ripened and 1-MCP (1-methylcyclopropene)-treated fruit groups during fruit ripening. These results indicate that the 14-3-3 proteins might be involved in various regulatory processes of banana fruit ripening. Further studies will mainly focus on revealing the detailed biological mechanisms of these four 14-3-3 genes in regulating banana fruit postharvest ripening.
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References
Bai MY, Zhang LY, Gampala SS, Zhu SW, Song WY, Chong K, Wang ZY (2007) Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in rice. Proc Natl Acad Sci USA 104:13839–13844
Cao A, Jain A, Baldwin JC, Raghothama KG (2007) Phosphate differentially regulates 14-3-3 family members and GRF9 plays a role in Pi-starvation induced responses. Planta 226:1219–1230
Chang IF, Curran A, Woolsey R, Quilici D, Cushman JC, Mittler R, Harmon A, Harper JF (2009) Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana. Proteomics 9:2967–2985
Chevalier D, Morris ER, Walker JC (2009) 14-3-3 and FHA domains mediate phosphoprotein interactions. Annu Rev Plant Biol 60:67–91
Gampala SS, Kim TW, He JX, Tang W, Deng Z, Bai MY, Guan S, Lalonde S, Sun Y, Gendron JM, Chen H, Shibagaki N, Ferl RJ, Ehrhardt D, Chong K, Burlingame AL, Wang ZY (2007) An essential role for 14-3-3 proteins in brassinosteroid signal transduction in Arabidopsis. Dev Cell 13:177–189
Huang FC, Do YY, Huang PL (2006) Genomic organization of a diverse ACC synthase gene family in banana and expression characteristics of the gene member involved in ripening of banana fruits. J Agric Food Chem 54:3859–3868
Inaba A, Liu X, Yokotani N, Yamane M, Lu WJ, Nakano R, Kubo Y (2007) Differential feedback regulation of ethylene biosynthesis in pulp and peel tissues of banana fruit. J Exp Bot 58:1047–1057
Ishida S, Fukazawa J, Yuasa T, Takahashi Y (2004) Involvement of 14-3-3 signaling protein binding in the functional regulation of the transcriptional activator REPRESSION OF SHOOT GROWTH by gibberellins. Plant Cell 16:2641–2651
Ishida S, Yuasa T, Nakata M, Takahashi Y (2008) A tobacco calcium-dependent protein kinase, CDPK1, regulates the transcription factor REPRESSION OF SHOOT GROWTH in response to gibberellins. Plant Cell 20:3273–3288
Liu X, Shiomi S, Nakatsuka A, Kubo Y, Nakamura R, Inaba A (1999) Characterization of ethylene biosynthesis associated with ripening in banana fruit. Plant Physiol 121:1257–1266
Liu J, Xu B, Hu L, Li M, Su W, Wu J, Yang J, Jin Z (2009) Involvement of a banana MADS-box transcription factor gene in ethylene-induced fruit ripening. Plant Cell Rep 28:103–111
Lohani S, Trivedi PK, Nath P (2004) Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana: effect of 1-MCP, ABA and IAA. Postharvest Biol Technol 31:119–126
Maraschin Sde F, Lamers GE, de Pater BS, Spaink HP, Wang M (2003) 14-3-3 isoforms and pattern formation during barley microspore embryogenesis. J Exp Bot 54:1033–1043
Mbéguié-A-Mbéguié D, Hubert O, Fils-Lycaon B, Chillet M, Baurens FC (2008) EIN3-like gene expression during fruit ripening of Cavendish banana (Musa acuminata cv. Grande naine). Physiol Plant 133:435–448
Paul AL, Sehnke PC, Ferl RJ (2005) Isoform-specific subcellular localization among 14-3-3 proteins in Arabidopsis seems to be driven by client interactions. Mol Biol Cell 16:1735–1743
Pua EC, Chandramouli S, Han P, Liu P (2003) Malate synthase gene expression during fruit ripening of Cavendish banana (Musa acuminata cv Williams). J Exp Bot 54:309–316
Roberts MR (2000) Regulatory 14-3-3 protein–protein interactions in plant cells. Curr Opin Plant Biol 3:400–405
Rosenquist M, Alsterfjord M, Larsson C, Sommarin M (2001) Data mining the Arabidopsis genome reveals fifteen 14-3-3 genes. Expression is demonstrated for two out of five novel genes. Plant Physiol 127:142–149
Roy Choudhury S, Roy S, Sengupta DN (2009) Characterization of cultivar differences in beta-1, 3 glucanase gene expression glucanase activity and fruit pulp softening rates during fruit ripening in three naturally occurring banana cultivars. Plant Cell Rep 28:1641–1653
Schoonheim PJ, Sinnige MP, Casaretto JA, Veiga H, Bunney TD, Quatrano RS, de Boer AH (2007a) 14-3-3 adaptor proteins are intermediates in ABA signal transduction during barley seed germination. Plant J 49:289–301
Schoonheim PJ, Veiga H, Pereira Dda C, Friso G, van Wijk KJ, de Boer AH (2007b) A comprehensive analysis of the 14-3-3 interactome in barley leaves using a complementary proteomics and two-hybrid approach. Plant Physiol 143(2):670–683
Sehnke PC, DeLille JM, Ferl RJ (2002) Consummating signal transduction: the role of 14-3-3 proteins in the completion of signal-induced transitions in protein activity. Plant Cell 14(Suppl):339–354
Sisler EC, Serek M (1997) Inhibitors of ethylene responses in plants at the receptor level: recent developments. Physiol Plant 100:577–582
Stover RH, Simmonds NM (1987) Bananas. Longman Science & Technology, London
Suzuki Y, Mae T, Makino A (2008) RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment. Biosci Biotechnol Biochem 72:1951–1953
Wang Y, Wu J, Xu BY, Liu JH, Zhang JB, Jia CH, Jin ZQ (2010) Cloning of an ADP-ribosylation factor gene from banana (Musa acuminata) and its expression patterns in postharvest ripening fruit. J Plant Physiol 167:989–995
Xu BY, Su W, Liu JH, Wang JB, Jin ZQ (2007) Differentially expressed cDNAs at the early stage of banana ripening identified by suppression subtractive hybridization and cDNA microarray. Planta 226:529–539
Yao Y, Du Y, Jiang L, Liu JY (2007a) Molecular analysis and expression patterns of the 14-3-3 gene family from Oryza sativa. J Biochem Mol Biol 40:349–357
Yao Y, Du Y, Jiang L, Liu JY (2007b) Interaction between ACC synthase 1 and 14-3-3 proteins in rice: a new insight. Biochemistry (Mosc) 72:1003–1007
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This work was supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-32) and 863 Project (SQ2010AA1000687012).
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Communicated by W. Harwood.
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Li, MY., Xu, BY., Liu, JH. et al. Identification and expression analysis of four 14-3-3 genes during fruit ripening in banana (Musa acuminata L. AAA group, cv. Brazilian). Plant Cell Rep 31, 369–378 (2012). https://doi.org/10.1007/s00299-011-1172-1
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DOI: https://doi.org/10.1007/s00299-011-1172-1