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Isolation of rice dwarf mutants with ectopic deposition of phenolic components including lignin in parenchyma cell walls of internodes

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Abstract

Rice internodes must have the proper shape to support high-yielding panicles. The shape of internodes is controlled by various factors involved in their formation, such as developmental patterns, cell division, cell elongation, and cell wall biosynthesis. To understand the regulation of internode development, we screened dwarf mutants to identify those with a phenotype of ectopic deposits of phenolic components in parenchyma cell walls of internodes. We named these mutants ectopic deposition of phenolic components1 (edp1). Two alleles were identified, edp1-1 and edp1-2. Furthermore, these mutants showed disordered cell files in internode parenchyma. These abnormal phenotypes were very similar to that of a previously reported dwarf50 (d50) mutant. Genetic analyses of edp1 mutants revealed that the edp1 loci are distinct from d50. Our results indicate that analyses of edp1 mutants as well as the d50 mutant will be useful for understanding the molecular mechanisms behind ectopic deposition of cell wall phenolic components in internode parenchyma cells and the regulation of internode development.

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Acknowledgments

We thank Hikaru Sato (Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan) for providing the rice mutants.

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Authors and Affiliations

  1. Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Kanna Sato, Asuka Kawamura, Tsukasa Obara & Shinya Kajita

  2. Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-8-1 Harumi-cho, Fuchu, Tokyo, 183-8509, Japan

    Shinya Kawai

  3. Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan

    Hidemi Kitano

  4. College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan

    Yoshihiro Katayama

Authors
  1. Kanna Sato
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  2. Asuka Kawamura
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  3. Tsukasa Obara
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  4. Shinya Kawai
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  5. Shinya Kajita
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  6. Hidemi Kitano
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  7. Yoshihiro Katayama
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Correspondence to Kanna Sato.

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Communicated by H. Ebinuma.

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Sato, K., Kawamura, A., Obara, T. et al. Isolation of rice dwarf mutants with ectopic deposition of phenolic components including lignin in parenchyma cell walls of internodes. Plant Cell Rep 30, 2195–2205 (2011). https://doi.org/10.1007/s00299-011-1125-8

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  • DOI: https://doi.org/10.1007/s00299-011-1125-8

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