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Cloning, expression and physiological analysis of broccoli catalase gene and Chinese cabbage ascorbate peroxidase gene under heat stress

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Abstract

The objectives of this work were to clone the catalase (CAT) gene from broccoli (Brassica oleracea) and the ascorbate peroxidase (APX) gene from Chinese cabbage and measure the regulation of CAT and APX gene expressions under heat-stress conditions. Different genotypes responded differently to heat stress according to their various antioxidant enzymes and physiological parameters. CAT and APX gene expression profiles were well matched with the data for CAT and APX enzyme activities in the broccoli and Chinese cabbage plants, respectively. Full-length of the CAT and APX cDNA were 1,768 and 1,070 bp, respectively. A phylogenetic analysis of CAT and APX indicated that plant CATs and APXs diverged into two major clusters.

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Acknowledgments

We thank Lein Chong Chung, Cruciferae Breeding Unit, AVRDC-The World Vegetable Center, Tainan, Taiwan, for a generous gift of seeds of broccoli (Green Magic and Shin-Fa 1208), and Chinese cabbage (Rein-Nong 720 and ASVEG #2). This research was supported by a grant (95–97 AS-1.3.2.FD-Z2) from the Council of Agriculture, Taiwan, ROC. The authors are grateful to Ms. Shu-Yen Pi for typing and editing the manuscript.

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  1. Graduate Institute of Biotechnology, Chinese Culture University, 55 Hwa-Kong Road, Taipei, 111, Taiwan, ROC

    Kuan-Hung Lin, Ho-Chang Huang & Ching-Yun Lin

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Correspondence to Kuan-Hung Lin.

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Communicated by J. Zou.

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Lin, KH., Huang, HC. & Lin, CY. Cloning, expression and physiological analysis of broccoli catalase gene and Chinese cabbage ascorbate peroxidase gene under heat stress. Plant Cell Rep 29, 575–593 (2010). https://doi.org/10.1007/s00299-010-0846-4

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