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The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans

  • Genetic Transformation and Hybridization
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Abstract

We investigated selective culturing conditions for the production of transgenic soybeans. In this culturing system, we used the acetolactate synthase (ALS)-inhibiting herbicide-resistance gene derived from rice (Os-mALS gene) as a selectable marker gene instead of that derived from bacteria, which interfered with the cultivation and practical usage of transgenic crops. T1 soybeans grown from one regenerated plant after selection of the ALS-targeting pyrimidinyl carboxy (PC) herbicide bispyribac-sodium (BS) exhibited herbicide resistance, and the introduction and expression of the Os-mALS gene were confirmed by genetic analysis. The selective culturing system promoted by BS herbicide, in which the Os-mALS gene was used as a selectable marker, was proved to be applicable to the production of transgenic soybeans, despite the appearance of escaped soybean plants that did not contain the Os-mALS transgene.

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Acknowledgments

We thank Motoko Kobayashi (NARCT) for technical assistance and Motoshige Kawata (National Institute of Crop Science, NICS) and Shuji Yokoi (Iwate University) for useful advice. This work was funded by the Project for the Development of Innovative Transgenic Plants of the Ministry of Agriculture, Forestry and Fisheries of Japan.

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Authors and Affiliations

  1. National Agricultural Research Center for Tohoku Region (NARCT), Morioka, Iwate, 020-0198, Japan

    Makoto Tougou, Jun-ichi Sakai, Seiji Kanematsu & Soh Hidaka

  2. Laboratory of Plant Pathology, Iwate University, Morioka, Iwate, 020-8550, Japan

    Noriko Yamagishi

  3. Kyoto Prefectural Institute of Agricultural Biotechnology, Seika-cho, Soraku-Gun, Kyoto, 619-0244, Japan

    Noriyuki Furutani

  4. Life Science Research Institute, Kumiai Chemical Industry Co., Ltd., Kakegawa, Shizuoka, 436-0011, Japan

    Koichiro Kaku & Tsutomu Shimizu

  5. Laboratory of Plant Breeding, Iwate University, Morioka, Iwate, 020-8550, Japan

    Yoshihito Takahata

Authors
  1. Makoto Tougou
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  2. Noriko Yamagishi
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  3. Noriyuki Furutani
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  4. Koichiro Kaku
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  5. Tsutomu Shimizu
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  6. Yoshihito Takahata
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  7. Jun-ichi Sakai
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  8. Seiji Kanematsu
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  9. Soh Hidaka
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Corresponding author

Correspondence to Makoto Tougou.

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Communicated by J. R. Liu.

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Tougou, M., Yamagishi, N., Furutani, N. et al. The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans. Plant Cell Rep 28, 769–776 (2009). https://doi.org/10.1007/s00299-009-0679-1

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  • DOI: https://doi.org/10.1007/s00299-009-0679-1

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