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Cell number, cell growth, antheridiogenesis, and callose amount is reduced and atrophy induced by deoxyglucose in Anemia phyllitidis gametophytes

  • Cell Biology and Morphogenesis
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Abstract

Fluorescence staining and morphometrical measurements revealed that callose was a component of newly formed cell plates of symmetrically dividing cells and asymmetrically dividing antheridial mother cells during gibberellic acid-induced antheridiogenesis as well as in walls of young growing cells of Anemia phyllitidis gametophytes. Callose in cell walls forms granulations characteristic of pit fields with plasmodesmata. 2-deoxy-d-glucose (DDG), eliminated callose granulations and reduced its amount estimated by measurements of fluorescence intensity. This effect was accompanied by reduction of antheridia and cell numbers as well as size and atrophy of particular cells and whole gametophytes. It is suggested that inhibition of glucose metabolism and/or signalling, might decrease callose synthesis in A. phyllitidis gametophytes leading to its elimination from cell plates of dividing cells and from walls of differentiating ones as well as from plasmodesmata resulting in inhibition of cytokinesis, cell growth and disruption of the intercellular communication system, thus disturbing developmental programs and leading to cell death.

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Abbreviations

GA3 :

Gibberellic acid

DDG:

2-Deoxy-D-glucose

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Authors and Affiliations

  1. Department of Cytophysiology, University of Łódź, Pilarskiego 14, 90231, Łódź, Poland

    Andrzej Kaźmierczak

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  1. Andrzej Kaźmierczak
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Correspondence to Andrzej Kaźmierczak.

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Communicated by R. Rose.

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Kaźmierczak, A. Cell number, cell growth, antheridiogenesis, and callose amount is reduced and atrophy induced by deoxyglucose in Anemia phyllitidis gametophytes. Plant Cell Rep 27, 813–821 (2008). https://doi.org/10.1007/s00299-007-0501-x

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  • DOI: https://doi.org/10.1007/s00299-007-0501-x

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