Abstract
An efficient micropropagation system for Erigeron breviscapus (vant.) Hand. Mazz., an important medicinal plant for heart disease, has been developed. Shoot organogenesis occurred from E. breviscapus leaf explants inoculated on a medium supplemented with a combination of plant growth regulators. On average, 17 shoots per leaf explant were produced after 30 days when they were cultured on MS basal salts and vitamin medium containing 5 μM 6-benzylaminopurine (BAP) and 5 μM 1-naphthaleneacetic acid (NAA). All the regenerated shoots formed complete plantlets on a medium containing 2.5–10 μM indole-3-butyric acid (IBA) within 30 days, and 80.2% of the regenerated plantlets survived and grew vigorously in field conditions. Based on the variation in common peaks and the produced amount of the most important bioactive component, scutellarin, a high performance liquid chromatography (HPLC) fingerprinting system was developed for quality control of these micropropagated plants. Chemical constituents in E. breviscapus micropropagated plants varied during plant development from regeneration to maturation, the latter of which showed the most similar phytochemical profile in comparison with mother plants. The regeneration protocol and HPLC fingerprint analysis developed here provided a new approach to quality control of micropropagated plants producing secondary metabolites with significant implications for germplasm conservation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chinese Pharmacopoeia Commission (2005) Pharmacopoeia of the People’s Republic of China. Chemical Industry Press, Beijing, pp 100–101
Gao M, Gu M, Liu CZ (2006) Two-step purification of scutellarin from Erigeron breviscapus (vant.) Hand. Mazz. by high-speed counter-current chromatography. J Chromatogr B 838:139–143
Gao M, Huang W, Liu CZ (2007) Microwave-assisted extraction of scutellarin from Erigeron breviscapus Hand-Mazz and its analysis by high-performance liquid chromatography. Anal Chim Acta 591:161–166
Guo B, Gao M, Liu CZ (2007) In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir. Plant Cell Rep 26:261–265
Huang JM, Weng WY, Huang XB, Ji YH, Chen E (2005) Pharmacokinetics of scutellarin and its aglycone conjugated metabolites in rats. Eur J Drug Metab Pharmacokinet 30:165–170
Li BY, Hu Y, Liang YZ, Xie PS (2004a) Quality evaluation of fingerprints of herbal medicine with chromatographic data. Anal Chim Acta 514:69–77
Li XL, Li YQ, Yan WM, Li HY, Xu H, Zheng XX, Guo DW, Tang LK (2004b) A study of the cardioprotective effect of breviscapine during hypoxia of cardiomyocytes in vitro and during myocardial infarction in vivo. Planta Med 70:1039–1044
Liu CZ, Murch S, El-Demerdash M, Saxena PK (2003a) Regeneration of Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep 21:525–530
Liu H, Yang XL, Ding JY, Feng YD, Xu HB (2003b) Antibacterial and antifungal activity of Erigeron breviscapus. Fitoterapia 74:387–389
Liu CZ, Murch S, Jain JC, Saxena PK (2004) Goldenseal (Hydrastis canadensis L.): in vitro regeneration for germplasm conservation and elimination of heavy metal contamination. In Vitro Cell Dev Biol Plant 70:73–79
Liu H, Yang XL, Tang R, Liu J, Xu HB (2005) Effect of scutellarin on nitric oxide production in early stages of neuron damage induced by hydrogen peroxide. Pharmacol Res 51:205–210
Liu HJ, Xu Y, Liu YJ, Liu CZ (2006) Plant regeneration from leaf explants of Rhodiola fastigiata. In Vitro Cell Dev Biol Plant 42:345–347
Liu CZ, Zhou HY, Yan Q (2007) Fingerprint analysis of Dioscorea nipponica by high-performance liquid chromatography with evaporative light scattering detection. Anal Chim Acta 582:61–68
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Murch S, Peiris SE, Liu CZ, Saxena PK (2004) In vitro conservation and propagation of medicinal plants. Biodiversity 5:19–24
World Health Organization (2003) WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants. WHO, Geneva
Zhu BH, Guan YY, He H, Lin MJ (1999) Erigeron breviscapus prevents defective endothelium-dependent relaxation in diabetic rat aorta. Life Sci 65:1553–1559
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by P. Lakshmanan.
Rights and permissions
About this article
Cite this article
Liu, CZ., Gao, M. & Guo, B. Plant regeneration of Erigeron breviscapus (vant.) Hand. Mazz. and its chromatographic fingerprint analysis for quality control. Plant Cell Rep 27, 39–45 (2008). https://doi.org/10.1007/s00299-007-0466-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-007-0466-9