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Analyses of the floral organ morphogenesis and the differentially expressed genes of an apetalous flower mutant in Brassica napus

  • Cell Biology and Morphogenesis
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Abstract

The floral organ morphogenesis of the apetalous flower mutant Apet33-10 in Brassica napus was investigated and the result showed that all the floral organ morphogenesis was normal except that petal primordium was not observed during flower development. Eighteen genes were found to be down regulated in early floral buds (less than 200 μm in length) of Apet33-10 at the stage of floral organ initiation by means of suppressive subtraction hybridization (SSH) and RT-PCR. These genes were involved in petal identity, calcium iron signal transduction, mRNA processing, protein synthesis and degradation, construction of cytoskeleton, hydrogen transportation, nucleic acid binding, alkaloid biosynthesis and unknown function. Three overall coding region cDNAs of APETALA3 (AP3) gene, BnAP3-2, BnAP3-3 and BnAP3-4 were obtained by RT-PCR, respectively. Real-time quantitative PCR analysis showed that the expression ratio among BnAP3-2, BnAP3-3 and BnAP3-4 was 3.67:3.68:1 in early floral buds of wild type Pet33-10. The expression level of BnAP3-2, BnAP3-3 and BnAP3-4 in early floral buds of Apet33-10 was down-regulated to 36.6, 28.3 and 66.8% with the comparison of that of wild type, respectively, and the overall expression level of AP3 genes in apetalous mutant amounted to 45.0% of that in wild type. The difference in the expression level of each AP3 gene in stamen between apetalous and wild type lines was not significant. It is suggested that lower abundant expression of AP3 genes during the early flower development might be enough for stamen primordium initiation, but not enough for petal primordium initiation in the apetalous line Apet33-10.

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Abbreviations

AP3 :

APETALA3

PCR:

Polymerase chain reaction

RT:

Reverse transcriptase

SSH:

Subtractive suppression hybridization

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Acknowledgments

We thank Dr Q. S. Liu, professor of Sichuan University, for reviewing the manuscript. This work was supported by the National Natural Science Foundation of China (Grant Nos. 30571174).

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  1. Key Laboratory of Ministry of Education for Bio-resources and Eco-environment, College of Life Science, Sichuan University, Chengdu, 610064, People’s Republic of China

    Y. T. Zhou, H. Y. Wang, L. Zhou, M. P. Wang, H. P. Li, M. L. Wang & Y. Zhao

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  1. Y. T. Zhou
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  2. H. Y. Wang
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  3. L. Zhou
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  4. M. P. Wang
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Correspondence to Y. Zhao.

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Communicated by M.C. Jordan.

Y.T. Zhou and H.Y. Wang are committed as the first author.

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Zhou, Y.T., Wang, H.Y., Zhou, L. et al. Analyses of the floral organ morphogenesis and the differentially expressed genes of an apetalous flower mutant in Brassica napus . Plant Cell Rep 27, 9–20 (2008). https://doi.org/10.1007/s00299-007-0426-4

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  • DOI: https://doi.org/10.1007/s00299-007-0426-4

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